Article
Biochemical Research Methods
Shuwen Hua, Quanshou Feng, Zehu Xie, Haijiao Mao, Yuping Zhou, Yinghua Yan, Chuan-Fan Ding
Summary: In this study, a novel hydrophilic material COF-S@Au@GC was developed to detect glycosylated exosomes in human serum. The material showed excellent detection performance and selectivity, and the glycopeptides enriched by COF-S@Au@GC can be detected by nano liquid chromatography with tandem mass spectrometry (LC-MS/MS), providing a new method for functional analysis of exosomes.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Physical
Qiongyuan Zhang, Jiayi Wu, Wuceng Niu, Jianjiang Xue
Summary: This study reports that the PTX-3 biosensor decorated with MWCNTs and AuNWs can achieve ultrasensitive detection of PTX-3 in human serum, with a wide range of detection, high sensitivity, and low detection limits. The stability of gold nanomaterials and the large electroactive surface area of MWCNTs improve the stability and repeatability of the sensor.
Article
Environmental Sciences
Krassimira Ilieva-Makulec, Anna Augustyniuk-Kram, Izabella Olejniczak, Kamil Karaban, Marek Nowicki, Tomasz Runka, Andrzej Kulczycki, Jaroslaw Kaluzny
Summary: The study found that multiwalled carbon nanotubes had a potential impact on soil organisms, especially with higher sensitivity in nematodes. Contamination by CNTs significantly affected the dynamics and diversity of soil fauna and microbial communities, potentially leading to long-term consequences for soil functioning and nutrient cycling.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Zehu Xie, Yinghua Yan, Keqi Tang, Chuan-Fan Ding
Summary: This study successfully prepared a novel COF material with high sensitivity and selectivity through post-synthesis modification, which showed significant enrichment effect on glycopeptides and is expected to be applied in glycoproteomics research.
Article
Environmental Sciences
Ifeoluwa O. Awogbindin, Ikenna C. Maduako, Isaac A. Adedara, Solomon E. Owumi, Akinola O. Ajeleti, Olatunde Owoeye, Anita K. Patlolla, Paul B. Tchounwou, Ebenezer O. Farombi
Summary: Studies have shown that exposure to multiwalled carbon nanotubes (MWCNTs) can impair liver and kidney function in rats through oxidative stress and inflammation, while treatment with kolaviron (KV) can mitigate these effects by its anti-inflammatory and antioxidant properties.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Liang Shan, Han Gao, Jing Zhang, Wentao Li, Yue Su, Yinlong Guo
Summary: This study combined MALDI-TOF MS with EM technology to directly analyze exosomal proteins in human plasma and serum. The results showed that EM had higher sensitivity in the high-mass range and characteristic exosomal proteins were identified. Furthermore, when using EM as a detector, there was a good linear relationship between peak intensity and concentration.
Article
Biochemistry & Molecular Biology
Francesca Scebba, Stefano Salvadori, Silvia Cateni, Paola Mantellini, Francesca Carozzi, Simonetta Bisanzi, Cristina Sani, Marzia Robotti, Ivana Barravecchia, Francesca Martella, Valentina Colla, Debora Angeloni
Summary: Ovarian cancer (OC) is a deadly gynecological cancer, and early diagnosis is crucial for a cure. Currently, a reliable screening method for early detection of OC is lacking. In this study, saliva samples were analyzed using proteomics to identify candidate biomarkers for OC. The results show that saliva is an informative test fluid for early detection of OC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Byungcheon Yoo, Ziwei Xu, Feng Ding
Summary: High-pressure carbon monoxide (HiPCO) synthesized single-walled carbon nanotubes (SWCNTs) were heat treated at high temperatures, resulting in an increase in diameter and the formation of double-, triple-, and multi-walled carbon nanotubes (MWCNTs). Most MWCNTs were found to have an odd number of walls, in accordance with the mechanism of SWCNT bundle coalescence.
Article
Biochemical Research Methods
Bin Liu, Yinghua Yan, Hongze Liang, Keqi Tang, Chuan-Fan Ding
Summary: Researchers developed a green strategy to prepare carbonaceous spheres rich in phosphoric acid groups with D-Glucose 6-phosphate sodium salt as a sole carbon source. The carbonaceous spheres were further functionalized with Ti4+ to obtain excellent performance in selective capture of phosphopeptides. The CS-Ti4+ showed great potential in mass spectrometry-based phosphoproteome studies by capturing low-abundance phosphopeptides from human saliva.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Davide Nardone, Angelo Ciaramella, Mariangela Cerreta, Salvatore Pulcrano, Gian C. Bellenchi, Linda Leone, Giuseppe Manco, Ferdinando Febbraio
Summary: SELDI-TOF mass spectrometry is used for protein analysis and biomarker screening, with SELYMATRA being a web application developed to automate identification processes and predict proteins in complex mixtures.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Siyuan Kong, Quanqing Zhang, Lujie Yang, Yuanyu Huang, Mingqi Liu, Guoquan Yan, Huanhuan Zhao, Mengxi Wu, Xiangmin Zhang, Pengyuan Yang, Weiqian Cao
Summary: A new enrichment strategy using a boronic acid-functionalized mesoporous graphene-silica composite is proposed for isolating intact glycopeptides, showing effectiveness and specificity in glycosylation analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Joanna Matysiak, Eliza Matuszewska, Marek L. Kowalski, Slawomir W. Kosinski, Ewa Smorawska-Sabanty, Jan Matysiak
Summary: Venom immunotherapy (VIT) in allergic patients is closely associated with changes in serum proteomic patterns, particularly involving complement proteins and myosin, with major proteomic changes detected in high responder group, suggesting a direct response to VIT.
Article
Immunology
Zhi Zheng, Qingfeng Wei, Xianghui Wan, Xiaoming Zhong, Lijuan Liu, Jiquan Zeng, Lihua Mao, Xiaojian Han, Fangfang Tou, Jun Rao
Summary: In this study, integrated proteomics and metabolomics analyses were performed on serum samples from advanced CRC patients. Significant alterations in proteins and metabolites were found after treatment. The proteomics analysis identified changes in cancer-related pathways and immune response pathways, while the metabolomics analysis revealed changes in pathways associated with lipid metabolism. The results of this study not only expanded our understanding of the molecular basis of advanced CRC but also provided potential novel therapeutic targets for CRC treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Jie Li, Anping Xu, Weijie Xie, Bowen Li, Cunliang Yan, Yong Xia, Chao Liang, Ling Ji
Summary: A new MALDI-TOF Mass spectrometry-based method has been developed for rapid screening of M-proteins in human serum, showing high analytical performance and throughput as a new choice for the diagnosis of plasma cell dyscrasias.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Analytical
Jiani Wu, Xueting Jin, Canhong Zhu, Yinghua Yan, Chuan-Fan Ding, Keqi Tang
Summary: A novel zwitterionic hydrophilic metal-organic framework (MOF)-functionalized material, PEI-ZIF-8@Au@GSH, demonstrated excellent performance in capturing glycopeptides with high sensitivity and outstanding enrichment selectivity by grafting GSH and Au onto the base material, PEI-ZIF-8.
Article
Chemistry, Analytical
Yimeng Geng, Sizhe Zhang, Yuxian Wang, Nengsheng Ye, Yuhong Xiang
Summary: A fluorescent switching based on BSA-stabilized gold nanoclusters and kanamycin aptamer was developed for the detection of Kana. The method achieved a good linear response for Kana in the concentration range of 0.04 nM to 7.0 nM. The assay showed satisfactory accuracy and recovery for detecting Kana in milk matrix, offering a novel assay for the detection of small molecules.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Wenjing Sun, Xiaoyu Hu, Xiaoyang Meng, Yuhong Xiang, Nengsheng Ye
Summary: A MoS2/GO composite was synthesized and used as an adsorbent for the determination of four paraben preservatives, showing great enrichment capability. The method has potential for the detection of emerging contaminants with low cost and high sensitivity.
Article
Environmental Sciences
Wenjing Sun, Xiaoyu Hu, Yuhong Xiang, Nengsheng Ye
Summary: In this study, four types of COF materials were synthesized by an ultrasonic method for adsorption of five sulfonamides. Optimization and kinetic experiments revealed main adsorption mechanisms such as pore filling, hydrogen bond interactions, and electrostatic attraction.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemical Research Methods
Rui Zong, Xuan Wang, Han Yin, Zijian Li, Chuanlin Huang, Yuhong Xiang, Nengsheng Ye
Summary: A three-dimensional covalent organic framework 3D TpTAM was successfully synthesized via Schiff-base reaction and ultrasound-assisted method, and TpTAM-coated capillary columns were prepared for the separation of fluoroquinolones by capillary electrochromatography with high resolution and reproducibility. The column showed good stability and could be used for more than 100 runs without noticeable changes.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
Sizhe Zhang, Yimeng Geng, Xinyi Deng, Nengsheng Ye, Yuhong Xiang
Summary: In this study, gold nanoclusters with bovine serum albumin as a template were synthesized rapidly using microwave-assisted method, and manganese dioxide was generated on the surface of the gold nanoclusters to achieve quantitative detection of alkaline phosphatase. The developed method is simple and convenient, with high sensitivity and good selectivity, showing potential applications in clinical diagnosis.
Article
Chemistry, Analytical
Rui Zong, Han Yin, Yuhong Xiang, Lu Zhang, Nengsheng Ye
Summary: A fluorinated covalent organic framework, TFA-TAPB, was proposed for electrochromatographic separation. The TFA-TAPB-coated capillary columns exhibited satisfactory separation performance and reproducibility, indicating high potential for application in CEC separation.
Article
Chemistry, Applied
Xinyi Deng, Sizhe Zhang, Nengsheng Ye, Lu Zhang, Yuhong Xiang
Summary: This study successfully synthesized 6-SH-CD@Au NCs and applied them for the detection of chlortetracycline. The results showed that the fluorescent system had good linearity, low detection limit, and could be successfully applied in milk samples.
Article
Food Science & Technology
Xiaofei Wang, Lu Zhang, Nengsheng Ye, Yuhong Xiang
Summary: A new metal-organic framework heterostructure composite (MOF@MOF) was synthesized and used as a sensor for the detection of tetracycline (TC). The sensor showed rapid detection, sensitivity, and selectivity with a limit of detection of 60 nM. It also displayed good linearity and a wide linear range, and was successfully applied to determine TC in milk and beef samples.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Xiaofei Wang, Lu Zhang, Yuhong Xiang, Nengsheng Ye, Kehui Liu
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has become a powerful method for studying the spatial distribution of molecules. The thickness of tissue sections is crucial for obtaining high-quality imaging data. This study investigated the effect of tissue slice thickness on MALDI MSI detection.
Article
Engineering, Chemical
Wenjing Sun, Lu Zhang, Yuhong Xiang, Nengsheng Ye
Summary: In this study, 2D and 3D covalent organic frameworks (COFs) membranes were prepared using a layer-by-layer strategy. These COFs/nylon 6 membranes exhibited high water permeance, excellent dye rejection, low salt rejection, and good chemical stability and reusability. The differences between 2D COFs membrane and 3D COFs membrane were analyzed, providing a universal method for the synthesis of different types of COFs membranes and a reference for comparing the performance of 2D and 3D COFs membranes in the same field.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
Article
Chemistry, Analytical
Sizhe Zhang, Qi Pu, Xinyi Deng, Lu Zhang, Nengsheng Ye, Yuhong Xiang
Summary: A ratiometric fluorescence sensor was constructed based on BSA-AuNCs and 2,3-diaminophenazine for the accurate and sensitive detection of choline. The sensor utilized choline oxidase for improved selectivity and molybdenum disulfide nanosheets with peroxidase-like activity for shortened detection time. The sensor exhibited a linear range of 0.5-20 μM and 20-100 μM for choline detection, with a detection limit of 0.27 μM. It provided a more accurate detection result compared to single signal sensors by reducing error interference.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Chuanlin Huang, Wenjuan Zhou, Riliga Wu, Weijiang Guan, Nengsheng Ye
Summary: This review summarizes the roles of nanomaterials in chemiluminescence (CL) systems, mainly including their roles as catalysts, emitters, and carriers. It also reviews the nanomaterial-based CL probes for biosensing and bioimaging of reactive oxygen species (ROS) developed in the past five years. The review is expected to provide guidance for the design and development of nanomaterial-based CL probes and facilitate the wider application of CL analysis in ROS sensing and imaging in biological systems.
Article
Biochemical Research Methods
Han Yin, Ziyi Zhen, Weijie Ning, Lu Zhang, Yuhong Xiang, Nengsheng Ye
Summary: In this study, a three-dimensional fluorinated covalent organic framework (FCOF) JUC-515 was successfully synthesized and introduced into a capillary column. The effects of buffer solution concentration, organic additive content, and pH on the separation of fluoroquinolones were investigated. The JUC-515-coated capillary column showed good separation performance and reproducibility, making it a promising stationary phase for capillary electrochromatography.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Wenjing Sun, Xiaoyu Hu, Yuhong Xiang, Lu Zhang, Nengsheng Ye
Summary: In this study, a magnetic covalent organic framework (COFs) named Fe3O4@TpBD was rapidly synthesized using an ultrasonic method, which greatly reduced the synthesis time. Fe3O4@TpBD was used as a magnetic solid-phase extraction (MSPE) adsorbent for the extraction and enrichment of sulfonamides with low limits of detection and good stability. The MSPE method was successfully applied to the determination of sulfonamides in shrimp samples.
Article
Biochemical Research Methods
Xuting Liu, Yi Zhen, Nengsheng Ye, Lu Zhang
Summary: This study reports a microfluidics-assembled tool for miRNA detection based on the regulation of DNA locked and unlocked states. The method utilizes microfluidic techniques and specific cleavage for signal amplification, enabling the detection of miRNA in complex samples.
