期刊
ANALYTICAL CHEMISTRY
卷 90, 期 18, 页码 10664-10669出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b01301
关键词
-
资金
- NIH [R01GM117191, R01DK071801, P41GM108538, R21AG05537701, S10RR029531]
- Office of the Vice Chancellor for Research and Graduate Education at UW-Madison
- Wisconsin Alumni Research Foundation (WARF)
- UW-Madison School of Pharmacy
Multiplex isobaric tags have become valuable tools for high-throughput quantitative analysis of complex biological samples in discovery-based proteomics studies. Hybrid labeling strategies that pair stable isotope mass difference labeling with multiplex isobaric tag-based quantification further facilitate these studies by greatly increasing multiplexing capability. In this work, we present a cost-effective chemical labeling approach that couples duplex stable isotope dimethyl labeling with our custom 12-plex N,N-dimethyl leucine (DiLeu) isobaric tags in a combined precursor isotopic labeling and isobaric tagging (cPILOT) strategy that is compatible with a wide variety of biological samples and permits 24-plex quantification in a single LC-MS/MS experiment. We demonstrate the utility of the DiLeu cPILOT approach by labeling yeast digests and performing prrof-of-principle quantification experiments on the Orbitrap Fusion Lumos.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据