Article
Biotechnology & Applied Microbiology
Wei Yang, Zheng Zhou, Haiping Wu, Changjin Liu, Bo Shen, Shijia Ding, Yonglie Zhou
Summary: A dual amplified electrochemiluminescence immunosensor was developed for ultrasensitive detection of anti-MPO, using PtCo/CdS@GO luminophores and K2S2O8/H2O2 coreactants. The novel signal amplification labels demonstrated excellent peroxidase-like catalytic activity and high-efficiency luminescence performance, leading to a low detection limit and good concentration linearity for anti-MPO detection. This study not only successfully detected anti-MPO in clinical serum samples, but also provided a new concept for designing high-performance luminophores with wide potential for biomarkers detection in clinical diagnosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shyang Pei Hong, Syazana Abdullah Lim, Natasha Ann Keasberry, Minhaz Uddin Ahmed
Summary: This work presents the development of an electrochemiluminescence immunosensor using a novel nanostructured composite of titanium dioxide and gold nanourchins for the detection of protein allergen Ovomucoid found in eggs. The sensor exhibited a low limit of detection, wide linear range, and excellent selectivity, reproducibility, and recovery rates in real food samples.
BIOELECTROCHEMISTRY
(2022)
Article
Biochemical Research Methods
Min Yin, Yan Wang, Xuwen Gao, Shichao Du, Yan Cheng, Shuai Yu, Guizheng Zou, Fumin Xue
Summary: A near-infrared electrochemiluminescence immunosensor was developed using AgInS2/ZnS nanocrystals as environmentally-friendly tags for sensitive and selective detection of CA125. The platform showed efficient detection capabilities with a detection limit of 1 x 10(-6) U/mL, offering potential for early diagnosis and treatment of ovarian cancer.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaoyi Lv, Xiaoyun Xu, Tian Miao, Xufeng Zang, Chao Geng, Yanping Li, Bo Cui, Yishan Fang
Summary: Fluorescent dyes are potential candidates for electrochemiluminescence reagents, but their application is limited due to self-absorption effects. A novel ECL emitter BPYHBF was developed for labeling competitors in immunosensors, along with a sensing substrate MWCNTs/Fc-MOF to improve sensor sensitivity and reliability. The ratiometric competitive immunosensor was successfully prepared for ultrasensitive detection of AFB1, with excellent sensitivity, accuracy, and reproducibility demonstrated in actual samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Cristina Gutierrez-Sanchez, Ana M. Villa-Manso, Monica Revenga-Parra, Felix Pariente, Encarnacion Lorenzo
Summary: Given the importance of fast, efficient, and cost-effective detection of SARS-CoV-2 in wastewater to control the pandemic, a new method based on a carbon nanodots-amplified electrochemiluminescence immunosensor has been proposed. The immunosensor showed high specificity and stability, with a detection limit of 1.2 pg/mL, and successfully detected SARS-CoV-2 in river and urban wastewater. This sensing platform provides a feasible and reliable tool for early virus detection and protection of the population.
Article
Chemistry, Analytical
Mengyuan Wang, Huixiao Li, Yadi Wang, Xiaodie Hu, Shujun Fang, Huili Ma, Wei Li, Dianlei Wang, Zhili Han
Summary: In this study, gold nanoclusters (AuNCs) were combined with a soft chitosan (CS) nanogel matrix (AuNCs@CS) using a simple one-pot approach. The resulting AuNCs@CS nanogels showed a 2.4-fold enhancement in electrochemiluminescence (ECL) intensity compared to bare AuNCs. This enhancement was attributed to increased Au(0) content, the confinement effect of the chitosan nanogels, and the prolonged excited state lifetime of AuNCs. The AuNCs@CS nanogels were used to develop a label-free ECL immunosensor for ultrasensitive detection of aflatoxin B1 (AFB1) with a significantly lower detection limit compared to previous ECL biosensors.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Si Yu Xiao, Shu Jun Zhen, Cheng Zhi Huang, Yuan Fang Li
Summary: A novel ultrasensitive ratiometric electrochemiluminescence biosensor was proposed for quickly identifying the toxicity of Bacillus anthracis by detecting the atxA mRNA. The sensor utilized a competitive mechanism and double amplified signal ways, achieving a wide linear relationship range and low detection limit. The strategy showed accessible operation, favorable performance, and high universality, making it potentially useful for analyzing other bacterial mRNAs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xinrong Shao, Xianzhen Song, Xin Liu, Liangguo Yan, Lei Liu, Dawei Fan, Qin Wei, Huangxian Ju
Summary: A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was successfully fabricated for highly sensitive detection of procalcitonin (PCT), demonstrating great potential for clinical applications.
Article
Chemistry, Analytical
Nuo Zhang, Shanghua Liu, Yue Jia, Guanhui Zhao, Xiaoyi Duan, Yaoguang Wang, Daopeng Zhang, Qin Wei
Summary: A novel electrochemiluminescence immunosensor was developed for sensitive detection of amyloid ss-protein. The sensor showed high signal intensity and low detection limit by using specific materials and labels. This strategy is of great significance for early diagnosis and prevention of Alzheimer's disease and detection of other disease markers.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Yi Xiao, Suhua Chen, Simeng Zhang, Guofang Wang, Haomin Yi, Gui-Zhong Xin, Xiaoping Yang
Summary: This study presents a mesoporous silica-mediated controllable electrochemiluminescence quenching immunosensor for straightforward and accurate measurement of immunological biomarkers. The sensitivity can be enhanced and the detection range can be broadened by tuning the ECL quenching, offering a new avenue for physiological and clinical implications of immunological biomarkers with a wide range of applications.
Article
Chemistry, Multidisciplinary
Jiajia Wang, Xin Hua, Baokang Jin
Summary: A facile in situ fabrication method for chitosan/polythiophene/CdTe nanocomposite was developed and connected with anti-CEA for electrochemical detection of carcinoembryonic antigen. The resulting nanocomposite showed high selectivity and excellent sensitivity, with a potential application in clinical tumor detection.
Article
Chemistry, Analytical
Lingya Ning, Yumin Bai, Zixiao Wang, Wenjun Wen, Junping Wang
Summary: A novel and rapid electro-chemiluminescence (ECL) immunosensor was successfully fabricated for ultrasensitive detection of 3-NT. The sensor utilized gold nanoparticles as the luminophore and electropolymerized polyaniline to promote the luminescence of the nanoparticles. It exhibited good analytical performance for 3-NT detection with a wide linear detection range and low detection limit, and had high recovery rate in practical sample applications.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Xiaoyi Lv, Mengmeng Bi, Xiaoyun Xu, Yanping Li, Chao Geng, Bo Cui, Yishan Fang
Summary: A novel ratiometric sandwich immunosensor for CYFRA21-1 detection was fabricated based on TPAPCN and 3D-GN/PtNPs/Th, achieving rapid and reliable detection through the ratio between ECL and electrochemical signals. The prepared sensor exhibited good linearity in the range of 50 fg/mL to 1 ng/mL with a detection limit as low as 16 fg/mL, showing significant application in clinical monitoring and biomolecules detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jiawei Gong, Tongtong Zhang, Tao Luo, Xuan Luo, Fei Yan, Weizhong Tang, Jiyang Liu
Summary: In this study, a novel solid-state electrochemiluminescence (ECL) platform was developed for rapid and ultra-sensitive detection of SARS-CoV-2 antibody. The platform utilizes bipolar silica nanochannel array to stably confine the ECL probe and achieve reliable detection of SARS-CoV-2 IgG.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Xiaochun Zhu, Yuxi Song, Xiaofeng Wang, Ying Zhou, Yaqin Chai, Ruo Yuan
Summary: In this study, methionine (Met)/N-acetyl-L-cysteine (NAC) templated copper nanoclusters (Met/NAC-Cu NCs) with tunable near-infrared region (NIR) electrochemiluminescence (ECL) emission wavelength were synthesized for ultrasensitive detection of matrix metalloproteinase-2 (MMP-2). The NAC played the role of template and reductant, while Met regulated the surface defect of Cu NCs to control the ECL emission wavelength. Additionally, a rapid target triggered catalyst hairpin assembly (CHA) recycling amplification strategy was developed to enhance signal amplification efficiency and reaction rate. A super-sensitive ECL biosensor was fabricated using Met/NAC-Cu NCs as the emitter, with a detection limit (LOD) as low as 1.65 fg/mL, and successfully applied for detecting MMP-2 from Hela and MCF-7 cancer cells.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Defen Feng, Xuecai Tan, Yeyu Wu, Chenhao Ai, Yanni Luo, Quanyou Chen, Heyou Han
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Analytical
Jinjie Li, Heng Yan, Xuecai Tan, Zhicheng Lu, Heyou Han
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Defen Feng, Penghui Li, Xuecai Tan, Wu Yeyu, Fucun Wei, Fangkai Du, Chenhao Ai, Yanni Luo, Quanyou Chen, Heyou Han
ANALYTICA CHIMICA ACTA
(2020)
Article
Chemistry, Analytical
Wenjing Wang, Mingbo Shu, Axiu Nie, Heyou Han
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Multidisciplinary
Zhaohui Zhang, Hui Liu, Qingzhu Sun, Feng Shao, Qingyan Pan, Tao Zhuang, Yingjie Zhao
Article
Multidisciplinary Sciences
Feng Shao, Wei Wang, Weimin Yang, Zhilin Yang, Yao Zhang, Jinggang Lan, A. Dieter Schlueter, Renato Zenobi
Summary: This study investigates plasmon-induced [4+4]-cycloaddition polymerization within anthracene-based monomer monolayers on Au(111) using TERS imaging. The experimental results combined with DFT calculations show that this two-dimensional polymerization occurs via a hot electron tunneling mechanism on Au(111).
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Feng Shao, Liqing Zheng, Jinggang Lan, Renato Zenobi
Summary: In this study, the local information of binary self-assembled monolayers (SAMs) on Au(111) coadsorbed from an equimolar mixture of p-cyanobenzenethiol (pCTP) and p-aminothiophenol (pATP) was investigated using tip-enhanced Raman spectroscopy (TERS) and density functional theory (DFT) calculations. The results revealed the coadsorption behavior, phase segregation, plasmon-induced photocatalysis, and solvation effects of the binary SAMs.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Feng Shao, Jun Kit Wong, Qi Hang Low, Marcella Iannuzzi, Jingguo Li, Jinggang Lan
Summary: This study investigates the mechanism of CO reduction reaction on Cu single-crystal surfaces using in situ spectroscopy and ab initio molecular dynamics calculations. The intermediate species and Cu-O-ad/Cu-OHad species during CO adsorption, dimerization, oxidation, and hydrogenation were identified. The accurate vibrational frequencies of these intermediates were determined using AIMD simulations. These findings provide important insights into the CO(2)RR process and offer a spectroscopic tool for future mechanistic studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Feng Shao, Zhaoming Xia, Futian You, Jun Kit Wong, Qi Hang Low, Hai Xiao, Boon Siang Yeo
Summary: In this study, we investigated the CO reduction reaction on Cu single-crystal surfaces using in situ electrochemical spectroscopy and density functional theory calculations. We identified coadsorbed and structure-/potential-dependent surface species, and found that the relative abundance of *OH follows a volcano trend with applied potentials in aqueous solutions. Combined with DFT calculations, we proposed that the surface H2O can serve as a strong proton donor for the first protonation step in both the C-1 and C-2 pathways of CORR at various applied potentials in alkaline electrolytes, leaving adsorbed *OH on the surface.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Qingyan Pan, Siqi Chen, Chenyu Wu, Feng Shao, Jing Sun, Lishui Sun, Zhaohui Zhang, Yixiao Man, Zhibo Li, Lixia He, Yingjie Zhao
Summary: GTTY is a novel graphyne allotrope synthesized via bottom-up approach, exhibiting excellent semiconductor properties with a band gap of 1.3 eV and a high conductivity of 0.285 S m(-1) at room temperature. The structural analysis reveals a ninefold stacking structure with nanosheet morphology. Hole and electron mobilities in GTTY films reach up to 1.47 x 10(3) cm(2) V-1 S-1 and 2.98 x 10(3) cm(2) V-1 S-1, respectively, as measured by the space-charge-limited current (SCLC) method.
Article
Chemistry, Multidisciplinary
Renzeng Chen, Danbo Wang, Wenbo Hao, Feng Shao, Yingjie Zhao
Summary: Inspired by tessellation in daily life, a rigid triangular macrocyclic molecule containing anthracene was synthesized for pre-organization through pi-pi interactions. A 2D polymer monolayer was successfully prepared at the air/water interface through [4+4]-photocycloaddition.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Zhaohui Zhang, Chenyu Wu, Qingyan Pan, Feng Shao, Qingzhu Sun, Siqi Chen, Zhibo Li, Yingjie Zhao
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Guilin Zhu, Jiawei Liu, Shuo Li, Yunpeng Zuo, Dian Li, Heyou Han
ACS APPLIED ENERGY MATERIALS
(2019)
Article
Chemistry, Analytical
Feng Shao, Liqing Zheng, Jinggang Lan, Renato Zenobi
Summary: The study of binary thiolate self-assembled monolayers (SAMs) on metal surfaces provides insights into their coadsorption behavior and solvation effects. By employing tip-enhanced Raman spectroscopy (TERS) imaging and density functional theory (DFT) calculations, this research sheds light on the properties of these SAMs.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Tingting Li, Shuo Li, Yunpeng Zuo, Guilin Zhu, Heyou Han
MATERIALS TODAY ENERGY
(2019)