期刊
ANALYTICAL CHEMISTRY
卷 86, 期 8, 页码 3703-3707出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac500465e
关键词
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资金
- National Natural Science Foundation of China [21305023, 31270875]
- Beijing Municipal Natural Science Foundation [2144058]
- Chinese Academy of Science [YZ201217]
- State Key Development Program for Basic Research of China [2011CB915502]
- International Cooperation Project [0102010DFB33880]
- National Key Scientific Instrument and Equipment Development Projects [2012YQ04014005]
Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 x 10(4) peptide library. The method provides a solution for high efficiency peptide screening.
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