Article
Chemistry, Analytical
Min Qing, Zhe Sun, Lei Wang, Shi Zhe Du, Jiao Zhou, Qian Tang, Hong Qun Luo, Nian Bing Li
Summary: The study combines electrochemical aptasensors with the CRISPR/Cas12a strategy, utilizing BIDSD, RCA, and CRISPR/Cas12a mechanisms to achieve specific and sensitive detection of thrombin with a calculated limit of detection of 1.26 fM, while simplifying the operation steps by omitting the probe surface-immobilization procedure.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Ting Huang, Daozhong Zhu, Tong Li, Mengxu Sun, Guixun Chen, Yanxin Zhang, Jin-Xiang Chen, Xiaoyong Zou, Zong Dai, Jun Chen
Summary: This paper proposes a toehold mediated feedback rolling circle amplification (TMFRCA) strategy for label-free nucleic acid sensing. The TMFRCA method exhibits great sensitivity and specificity, making it a valuable tool for nucleic acid research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yuanyuan Zhang, Jian Wei, Jinwen Zhao, Ying He, Ruo Yuan, Shihong Chen
Summary: This study developed quadruple cascade strand displacement amplification (QC-SDA)-mediated polyfluorene derivative-based multiple fluorescence resonance energy transfer (FRET) for simultaneous determination of AD biomarkers miRNA-16 and amyloid-beta oligomer species (A beta Os). The method achieved high-throughput detections with high sensitivity and ease of operation.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Saiwei Wu, Abdu Ahmed Abdullah Al-Maskri, Qun Li, Jiatong Liu, Sheng Cai
Summary: A novel ligation-based loop-mediated isothermal amplification (LAMP) method was developed for miRNA detection. It utilized stem-loop structure DNA linker A/B probes to establish a rapid and ultrasensitive miRNA-LAMP system. This method can detect miRNA in a linear range of seven orders of magnitude, with a detection limit as low as 100 fM. It provides a new path for highly sensitive quantitative analysis of miRNA, which can bring convenience to clinical diagnosis and prognostic research.
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jayeon Song, Yong Ju, Soohyun Kim, Hansol Kim, Hyun Gyu Park
Summary: In this study, we developed a palindromic hyperbranched rolling circle amplification (PH-RCA) reaction for ultrasensitive detection of microRNAs. The PH-RCA reaction significantly enhances the sensitivity and reduces the reaction time within 30 minutes, making it a promising technique for miRNA detection.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Hyojin Lee, Hyobeen Lee, Sang-Hyun Hwang, Woong Jeong, Dong-Eun Kim
Summary: This study developed a new method for sensitive detection of SARS-CoV-2 viral RNA without thermal cycling. By using tandem isothermal gene amplification technology, this method can detect very low concentrations of viral RNA within 1 hour, and it has high sensitivity and accuracy for COVID-19 diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Zezhou Yang, Yu Guo, Jie Zhou, Fang Liu, Wenbin Liang, Yaqin Chai, Zhaohui Li, Ruo Yuan
Summary: In this study, an innovative fluorescent sensor was developed for precise and ultrasensitive detection and imaging of target miRNA-21. The sensor utilized a dextrous target-motivated polymerization/nicking DNA nanomachineries and a hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand displacement reaction (SDR) signal amplification approach. The technique achieved target recycling through polymerization/nicking DNA nanomachineries and HB-RCA amplification induced by the released transformation target. HB-RCA was exploited to increase the local concentration and collision efficiency of the templates and primers, generating multiple repeated DNA sequences as initiators for SDR, which improved the signal amplification multiplier. The technique effectively distinguished target miRNA-21 and detected drug-manipulative miRNA expression level abnormities. The proposed cascade nucleic acid amplification strategy could offer a promising pathway for ultrasensitive imaging of diverse biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ke Wang, Xinnan Bai, Yingying Xue, Xinyi Luo, Jianpei Dong, Gewei Yang, Kun Ma, Lu Huang, Jianhua Zhou, Jiasi Wang
Summary: Circular RNA (circRNA) is a non-coding RNA that shows promise as a biomarker for diagnosing and predicting diseases like cancer. Traditional methods like qRT-PCR are not suitable for accurately quantifying circRNA, but a new assay called droplet digital RT-HRCA (ddRT-HRCA) has been developed for sensitive and quantitative detection. This novel strategy using ddRT-HRCA paves the way for accurately measuring low-abundance circRNA, which could be used in liquid biopsies for early diagnosis and prognosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jianping Zheng, Xiaolin Xu, Hanning Zhu, Zhipeng Pan, Xianghui Li, Fang Luo, Zhenyu Lin
Summary: Target-induced differences in electrostatic interactions were utilized to develop a homogeneous electrochemical biosensor for flap endonuclease 1 (FEN1) detection. The sensor showed a linear relationship between signal change and logarithm of FEN1 concentration, with a low detection limit of 0.003 U/L. This study provides a label-free and homogeneous electrochemical platform for evaluating FEN1 activity.
Article
Chemistry, Analytical
Wenyan Jiang, Zhongping Chen, Jin Lu, Xue Ren, Yue Ma
Summary: This study developed a new detection method based on gold nanoparticles and CRISPR/Cas12a for highly sensitive detection of miRNA-143 as a noninvasive biomarker for prostate cancer diagnosis. The method exhibited outstanding specificity and sensitivity, and it is simple to operate, making it suitable for use in low-resource settings.
Article
Chemistry, Analytical
Gaihua Cao, Keyi Long, Yue Qiu, Yi Ma, Hui Qin, Danqun Huo, Mei Yang, Caihong Shen, Changjun Hou
Summary: A biosensor based on IPAR amplification mode and DSN was constructed for sensitive detection of FzD5 mRNA. The biosensor, utilizing the high amplification efficiency of IPAR mode, achieved a detection limit as low as 99 fM, showing promising potential in cancer marker detection.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Su Jiang, Meng Liu, Wei Tantai, Qinfeng Xu, Xiaoran Zou, Fei Ma, Chun-yang Zhang
Summary: We have developed an aptamer-mediated rolling circle amplification approach for the label-free and sensitive detection of histone-modifying enzyme activity. This method can achieve femtomolar sensitivity for the assay of histone acetyltransferase Tip60, and can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jiemei Zhao, Xiaoqin Zhao
Summary: This paper describes a simple and sensitive miRNA detection method based on dual signal recycles, which can be applied for early clinical diagnosis and biological research.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)