Article
Biochemical Research Methods
Nadja Mallock, Andrea Rabenstein, Peter Laux, Tobias Ruether, Christoph Hutzler, Maria Kristina Parr, Andreas Luch
Summary: A quantitative method based on LC-ESI-MS/MS was developed and validated for reliable determination of nicotine and its metabolites in blood plasma. The method showed good accuracy and precision for quantitation of nicotine, cotinine, and hydroxycotinine in plasma samples. Optimization of sample preparation and chromatographic conditions were crucial for successful application of the method.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Volha Yakimavets, Tian Qiu, Parinya Panuwet, Priya E. D'Souza, Patricia A. Brennan, Anne L. Dunlop, P. Barry Ryan, Dana Boyd Barr
Summary: To assess the combined health risks of co-exposure to tobacco and marijuana, researchers have developed an analytical method to simultaneously measure tobacco and marijuana metabolites. This method reduces laboratory costs and sample volume, making it suitable for large-scale sample analysis.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Paulo Roberto Xavier Tomaz, Thuane Sales Goncalves, Juliana Rocha Santos, Jaqueline Scholz, Tania Ogawa Abe, Patricia Viviane Gaya, Eduardo Costa Figueiredo, Henrique Dipe de Faria, Isarita Martins, Ana Miguel Fonseca Pego, Beatriz Aparecida Bismara, Mauricio Yonamine, Alexandre Costa Pereira, Paulo Caleb Lima Santos Junior
Summary: This study aimed to evaluate the application of nicotine metabolite ratio (NMR) in Brazilian smoking patients and analyze its association with smoking characteristics. The findings contribute to the development of personalized smoking cessation treatments and improvement of success rates.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jacek Baj, Wojciech Flieger, Dominika Przygodzka, Grzegorz Buszewicz, Grzegorz Teresinski, Magdalena Pizon, Ryszard Maciejewski, Jolanta Flieger
Summary: This study developed and compared two new chromatographic methods, HPLC-QQQ-MS/MS and RP-HPLC-DAD enriched with chaotropic additives, for the determination of nicotine and its major metabolites in human plasma. The results showed that HPLC-QQQ-MS/MS had lower limits of detection, while RP-HPLC-DAD enriched with chaotropic additives had higher extraction efficiency.
Article
Chemistry, Multidisciplinary
Baoyun Xia, Benjamin C. Blount, Lanqing Wang
Summary: A simple and sensitive method was developed to measure nicotine in the BAL fluid, which proved useful in detecting nicotine exposure in EVALI patients and similar illnesses.
Article
Environmental Sciences
Jenny Becam, Edouard Martin, Gildas Pouradier, Natalia Doudka, Caroline Solas, Romain Guilhaumou, Nicolas Fabresse
Summary: We present a case of accidental nicotine intoxication in a 22-year-old man working in an e-liquid manufacturing company. The patient spilled 300 mL of pure nicotine solution on his leg without protection, experiencing symptoms such as dizziness, nausea, and burning sensations. He recovered without treatment after five hours. This case highlights the importance of protective clothing when handling nicotine-containing liquid products.
Article
Biochemical Research Methods
Hideaki Ishii, Gary Ngai-Wa Leung, Shozo Yamashita, Shun-ichi Nagata, Asuka Kushiro, Satoshi Sakai, Kota Toju, Jun Okada, Kazumi Kawasaki, Kanichi Kusano, Isao Kijima-Suda
Summary: The use of nicotine stimulants in horses is generally banned due to potential nicotine exposure from accidental consumption of tobacco products. A comprehensive metabolic study of nicotine in horses was conducted, and potential biomarkers, such as trans-3'-hydroxycotinine and anatabine, were identified in equine urine and plasma to indicate recent tobacco product exposure.
DRUG TESTING AND ANALYSIS
(2022)
Article
Environmental Sciences
Zixuan Wang, Hui Shi, Ling Peng, Yue Zhou, Ying Wang, Fei Jiang
Summary: This study found gender-specific differences in the association between environmental tobacco smoke exposure biomarkers and developmental disorders. Only girls with low cotinine levels and high urinary NNAL levels had higher odds of having developmental disorders, while the effects of boys and NNAL exposure were additive.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Substance Abuse
Robert K. Cooper Jr, Martin C. Mahoney, Stephen T. Tiffany, Craig R. Colder, Rachel F. Tyndale, Larry W. Hawk Jr
Summary: People who metabolize nicotine more quickly are generally less successful at quitting smoking, but the mechanisms linking nicotine metabolism to smoking reinforcement and craving are unclear. This study found that in a sample of adults seeking to quit smoking, higher metabolism rate was not associated with greater smoking reinforcement or craving.
NICOTINE & TOBACCO RESEARCH
(2023)
Article
Environmental Sciences
Mohd Rehan, Ummer R. Zargar, Ishfaq A. Sheikh, Saif A. Alharthy, Majed N. Almashjary, Adel M. Abuzenadah, Mohd A. Beg
Summary: Tobacco/nicotine poses a significant threat to global public health and has harmful effects on various systems of the human body. This study found that nicotine and its metabolites can interfere with the binding of carrier proteins and hormones, leading to an imbalance in hormone transport and homeostasis in the blood circulation.
Article
Environmental Sciences
Elisabet Soderstrom, Torbjorn K. Nilsson, Jorn Schneede, Per-Magne Ueland, Oivind Midttun, Bjorn Gylling, Ingegerd Johansson, Johan Hultdin
Summary: This study investigated the impact of smoking and snus use on plasma homocysteine levels, finding that cotinine, creatinine, methylmalonic acid, and MTHFR T allele were positively associated with tHcy in smokers but not in snus users, suggesting that substances other than nicotine in tobacco smoke may contribute to differential effects on homocysteine status.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Hyun-Seung Lee, Mi-Ryung Chun, Soo-Youn Lee
Summary: Measuring multiple nicotine metabolites and total nicotine equivalents (TNE) is a more reliable method for verifying tobacco exposure compared to measuring single urinary cotinine alone. This measurement can be helpful for monitoring low-level or secondhand smoke exposure and for determining individual differences in nicotine metabolism.
Article
Public, Environmental & Occupational Health
Kaitlyn O. Browning, Michael J. DeSarno, Danielle R. Davis, Joanna M. Streck, Cecilia L. Bergeria, Roxanne F. Harfmann, Maria A. Parker, Sarah H. Heil, Stacey C. Sigmon, Diann E. Gaalema, Jennifer W. Tidey, Dustin C. Lee, Haley J. Tetreault, Stephen T. Higgins
Summary: This study uses multivariate regression to investigate the contribution of the reinforcing value of smoking and nicotine-dependence severity to individual differences in nicotine exposure levels among vulnerable populations. The results suggest that differences in reinforcing value may underpin a considerable portion of the variance in nicotine exposure accounted for by dependence severity.
PREVENTIVE MEDICINE
(2022)
Article
Environmental Sciences
Georg E. Matt, Ashley L. Merianos, Lara Stone, Chase Wullenweber, Penelope J. E. Quintana, Eunha Hoh, Nathan G. Dodder, Nicolas Lopez Galvez, E. Melinda Mahabee-Gittens
Summary: This study found that hand nicotine levels provide additional insights into children's exposure to tobacco smoke pollutants than reported child TSE and urinary biomarkers. Changes in hand nicotine levels show that consistent home smoking bans in homes of children of smokers can lower THS exposure. Hand nicotine levels may be influenced by the environmental settings in which they are collected.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Green & Sustainable Science & Technology
Marc W. Beutel, Thomas C. Harmon, Thomas E. Novotny, Jeremiah Mock, Michelle E. Gilmore, Stephen C. Hart, Samuel Traina, Srimanti Duttagupta, Andrew Brooks, Christopher L. Jerde, Eunha Hoh, Laurie C. van de Werfhorst, Van Butsic, Ariani C. Wartenberg, Patricia A. Holden
Summary: The impact of cigarette and e-cigarette contamination on the environment is significant, affecting various biota from bacteria to mammals. Chemical substances released from cigarette butts and e-cigarette components can be toxic to a wide range of organisms, highlighting the potential for large-scale environmental impacts of tobacco product waste. However, there are still critical gaps in understanding the full extent of these impacts on environmental health and ecosystem functioning.
Article
Biochemical Research Methods
Veronica Castro, Jose Benito Quintana, Javier Lopez-Vazquez, Nieves Carro, Julio Cobas, Denis Bilbao, Rafael Cela, Rosario Rodil
Summary: This study presented an optimized screening method using gas chromatography-electron ionization-high-resolution mass spectrometry (GC-EI-HRMS) and developed an accurate-mass HRMS library for the detection of harmful substances in mussels, achieving satisfactory detection results.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Benigno Jose Sieira, Rosario Rodil, Rafael Cela, Jose Benito Quintana, Rosa Montes
Summary: This study investigated the reaction of VP and PPE under UV radiation, finding high quantum yields and reactivity, leading to the formation of multiple transformation products. The toxicity of precursors and TPs was estimated using QSAR software tools, highlighting the need for experimental toxicity assays.
Article
Environmental Sciences
Bruna Castanheira, Larissa Otubo, Cristiano L. P. Oliveira, Rosa Montes, Jose Benito Quintana, Rosario Rodil, Sergio Brochsztain, Vitor J. P. Vilar, Antonio Carlos S. C. Teixeira
Summary: The photocatalytic activity of TiO2 nanoparticles supported on mesoporous silica SBA-15 was evaluated for the photodegradation of sulfadiazine. The 30% TiO2/SBA-15 sample showed the best performance in pure water solutions and also demonstrated higher SDZ degradation efficiency in urban secondary wastewater. Additionally, LC-MS analysis identified 65 other CECs in the wastewater sample, which were effectively removed by the photocatalytic system.
Article
Environmental Sciences
Mariana F. T. Sa, Veronica Castro, Ana Gomes, Daniela F. S. Morais, Rui V. P. S. Silva Braga, Isabel Saraiva, Bianca M. Souza-Chaves, Minkyu Park, Victoria Fernandez-Fernandez, Rosario Rodil, Rosa Montes, Jose Benito Quintana, Vitor J. P. Vilar
Summary: This study provides a comprehensive analysis of organic contaminants and dissolved organic matter in the sewer network, highlighting the impact of higher contamination areas on the performance of a municipal wastewater treatment plant. The presence of surfactants and their concentration in the influent contributed to the morphological changes in the activated sludge and affected the overall efficiency of the plant.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Rosa Montes, Sandra Mendez, Nieves Carro, Julio Cobas, Nelson Alves, Teresa Neuparth, Miguel Machado Santos, Jose Benito Quintana, Rosario Rodil
Summary: Contaminants of emerging concern (CECs) are a group of compounds with diverse origins that have recently garnered increasing environmental attention. The NOR-Water project aimed to identify the main CECs and their sources in the water environment of the Northern Portugal-Galicia transnational region. Through the use of liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS), a total of 343 chemicals were tentatively identified in the analyzed samples, with 153 classified as persistent, mobile, and toxic (PMT) and 23 as very persistent and very mobile (vMvP).
Article
Environmental Sciences
Andrea Estevez-Danta, Lubertus Bijlsma, Ricardo Capela, Rafael Cela, Alberto Celma, Felix Hernandez, Unax Lertxundi, Joao Matias, Rosa Montes, Gorka Orive, Ailette Prieto, Miguel M. Santos, Rosario Rodil, Jose Benito Quintana
Summary: This study investigated the use of illicit drugs and licit substances of abuse during the COVID-19 pandemic restrictions in several locations in Spain and Portugal using wastewater-based epidemiology. The results showed widespread use of substances such as cannabis and cocaine, with limited use of methamphetamine. The study also confirmed two instances of ecstasy dumping in the sewage network.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Susana Barros, Marta Ribeiro, Ana M. Coimbra, Marlene Pinheiro, Hugo Morais, Nelson Alves, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Miguel M. Santos, Teresa Neuparth
Summary: This study investigated the effects of metformin on zebrafish metabolism and other biological processes. The results indicate that environmentally relevant concentrations of metformin severely disrupt zebrafish metabolism and lead to disturbances in critical biochemical and molecular processes, as well as impacting other important pathways.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Susana Barros, Nelson Alves, Marlene Pinheiro, Marta Ribeiro, Hugo Morais, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Ana M. Coimbra, Miguel M. Santos, Teresa Neuparth
Summary: This study investigated the generational effects of the antidiabetic drug MET on zebrafish and its nonexposed offspring. The results showed that MET had significant effects on reproductive-related parameters in zebrafish, with parental exposure to MET impacting critical molecular processes and upregulating male vtg1 expression, indicating an estrogenic effect. The nonexposed offspring also experienced negative effects on survival and growth due to parental MET exposure.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Editorial Material
Engineering, Environmental
Unax Lertxundi, Saioa Domingo-Echaburu, Susana Barros, Miguel Machado Santos, Teresa Neuparth, Jose Benito Quintana, Rosario Rodil, Rosa Montes, Gorka Orive
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Carla Santos, Miguel Herraiz-Carbone, Engracia Lacasa, Cristina Saez, Rosa Montes, Jose Benito Quintana, Rosario Rodil, Ana I. Gomes, Vitor J. P. Vilar
Summary: This research explores the use of a tubular membrane photoreactor for continuous titration of Fe2+ to enhance the Photo-Fenton process for the tertiary treatment of urban wastewater. The study shows that the PF-like process exhibits superior performance in removing contaminants compared to traditional PF process, especially for melamine. The PF-like process also has advantages in terms of higher residual concentrations of Fe2+ and lower precipitation of PO43-, making it a promising option for tertiary treatment.
CHEMICAL ENGINEERING JOURNAL
(2023)
Letter
Environmental Sciences
Hans Peter H. Arp, Raoul Wolf, Sarah E. Hale, Sivani Baskaran, Juliane Gluege, Martin Scheringer, Xenia Trier, Ian T. Cousins, Harrie Timmer, Roberta Hofman-Caris, Anna Lennquist, Andre D. Bannink, Gerard J. Stroomberg, Rosa M. A. Sjerps, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Daniel Zahn, Herve Gallard, Tobias Mohr, Ivo Schliebner, Michael Neumann
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Rosa Montes, Sandra Mendez, Julio Cobas, Nieves Carro, Teresa Neuparth, Nelson Alves, Miguel M. Santos, Jose Benito Quintana, Rosario Rodil
Summary: This study examined the occurrence and fate of 52 emerging contaminants in transnational river basins, coastal areas, and wastewater treatment plants in Portugal and Spain. The results showed that these contaminants were widely present and were only partially removed by conventional treatment processes. These findings highlight the need for significant upgrades and coordination of wastewater treatment plants to meet future EU regulations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Pedro H. Presumido, Rosa Montes, Jose B. Quintana, Rosario Rodil, Manuel Feliciano, Gianluca Li Puma, Ana Gomes, Vitor J. P. Vilar
Summary: A tubular porous borosilicate membrane contactor was studied for ozone gas/water mass transfer and removal of contaminants in water. Increasing the gas and liquid flow rate improved the mass transfer efficiency, with the main resistances being in the water phase boundary layer and the membrane. The contactor showed good feasibility in removing contaminants, but photocatalytic ozonation did not significantly enhance treatment performance.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)