Article
Chemistry, Multidisciplinary
Oksana Y. Dudaryeva, Aurelia Bucciarelli, Giovanni Bovone, Florian Huwyler, Shibashish Jaydev, Nicolas Broguiere, Marwa al-Bayati, Marco Lutolf, Mark W. Tibbitt
Summary: The biophysical properties of the cellular microenvironment, such as stiffness and geometry, have been shown to influence cell function, with recent findings implicating 3D confinement as a significant regulator of cell behavior. This study demonstrates that the extent of 3D confinement affects cell viability and proliferation, leading to changes in nuclear morphology and YES-associated protein (YAP) localization. The effects of confinement on cell behavior scale with the degree of 3D confinement, highlighting the importance of 3D confinement as a regulator of cell function.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Nabila Yasmeen, Aneta Karpinska, Jakub Kalecki, Wlodzimierz Kutner, Karina Kwapiszewska, Piyush S. Sharma
Summary: Biocompatible polyacrylamide gel and core-shell nanoparticles were synthesized using a one-step electro-chemically initiated gelation. The core-shell nanoparticles exhibited superior support for complex 3D tissue structures.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Chengxun Su, Yon Jin Chuah, Hong Boon Ong, Hui Min Tay, Rinkoo Dalan, Han Wei Hou
Summary: Incorporation of ECM and hydrogel in microfluidic cell culture platforms is crucial for creating a physiological cell microenvironment and establishing 3D co-culture models. The developed hydrogel patterning method based on stepped heights allows for easy adaptation in both enclosed microchannels and open surfaces, making it suitable for in-gel cultures of larger 3D cellular spheroids or microtissues.
Review
Pharmacology & Pharmacy
Lisa Belfiore, Behnaz Aghaei, Andrew M. K. Law, Jeremy C. Dobrowolski, Lyndon J. Raftery, Angie D. Tjandra, Christine Yee, Alberto Piloni, Alexander Volkerling, Cameron J. Ferris, Martin Engel
Summary: 3D cell models are considered more accurate in predicting the efficacy of drug responses and are seen as more physiologically representative tools for in vitro modeling of in vivo tissues. In the future, 3D cell models will continue to play an important role in drug discovery applications.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Review
Oncology
Hande Ozkan, Deniz Gulfem Ozturk, Gozde Korkmaz
Summary: Transcriptional regulation of breast cancer tumorigenesis has been studied primarily in 2D culture models, but 3D cell culture models better mimic the in vivo tumor microenvironment and have great potential for breast cancer research.
Article
Biochemical Research Methods
Huichao Chai, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.
Article
Engineering, Biomedical
Jaeseung Youn, Junyeol Rhyou, Dohui Kim, Jisang Lee, Jeong-Won Choi, Tae-Eun Park, Dong Sung Kim
Summary: We propose a facile and leak-free method for bonding nanofiber (NF) membranes onto thermoplastic polymer (TP) platforms by localized thermal bonding, while preserving the nanofibrous structure of the membrane. The optimal preheating temperature was determined through a numerical simulation model and experimentally validated for bonding stability and durability. The applicability of this method was demonstrated on various combinations of TP materials and supporting substrate geometries. We expect this localized thermal bonding method to broaden the practical applications of functional NF membranes in biomedical fields.
MATERIALS TODAY BIO
(2023)
Article
Optics
Qiang Li, Huan Deng, Cuini Yang, Wei He, Feiyan Zhong
Summary: This paper proposes a locally controllable mixed 2D/3D display system and its image generation method. The system consists of a collimating backlight module and a light control module, which can locally control the 2D and 3D display states. An image generation method is also proposed, dividing the parallax image into target and residual image areas using a deep learning matting algorithm to generate a mixed light field image with 3D target image and high-resolution 2D residual image. Experimental results demonstrate the effectiveness of the system and image generation method.
Article
Multidisciplinary Sciences
Remy Elbez, Jeff Folz, Alan McLean, Hernan Roca, Joseph M. Labuz, Kenneth J. Pienta, Shuichi Takayama, Raoul Kopelman
Summary: The study introduces a novel method for measuring cell morphodynamics without the use of biomarkers, utilizing cell magnetorotation and machine learning to analyze real-time cell morphology dynamics. Through this method, researchers successfully addressed the broad heterogeneity of morphological phenotypes in cancer cell populations and enabled rapid screening and identification of invasive cells.
Article
Energy & Fuels
Amir Mohammad Norouzi, Fatemeh Pouranian, Arash Rabbani, Neil Fowler, Jon Gluyas, Vahid Niasar, Justin Ezekiel, Masoud Babaei
Summary: The performance of a CO2 thermosiphon system in three different widths of fluvial channels in a 3D sedimentary aquifer was analyzed. The presence of fluvial channels significantly increased the aquifer thermal depletion pace and reduced the system's performance. The CPG system with a CO2 injection rate of 50 kg/s and an I-P line parallel to the channels provided the maximum cycle operation time and the optimum performance in the heterogeneous cases of the study.
Article
Neurosciences
Doris Lam, Heather A. Enright, Jose Cadena, Vivek Kurien George, David A. Soscia, Angela C. Tooker, Michael Triplett, Sandra K. G. Peters, Piyush Karande, Alexander Ladd, Chandrakumar Bogguri, Elizabeth K. Wheeler, Nicholas O. Fischer
Summary: The study introduces a novel computational analytical pipeline to analyze 3D neural network activity. The results demonstrate differences in network activity within and between cross sections of the 3D hydrogel-based tissue, with a preference for GABA and/or glutamate synaptic transmission.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Chemistry, Analytical
Haiqiang Liu, Chen Yang, Bangbing Wang
Summary: A full PDMS micro-droplet chip for 3D cell culture was prepared using SLA light-curing 3D printing technology. The chip can be customized quickly for different experiments, saving time and capital costs. Experimental verification showed that the designed chip met the requirements and achieved precise control over the micro-droplets.
Article
Biotechnology & Applied Microbiology
Christoph Gruen, Jana Pfeifer, Gregor Liebsch, Eric Gottwald
Summary: Oxygen concentration is vital in (3D) cell culture, but the oxygen content in vitro is not comparable to the in vivo situation due to experimental conditions. Current measurement methods are limited to 2D cultures and lack accuracy in 3D cell culture. This paper presents a system using microthermoforming to generate oxygen-sensitive microcavity arrays for real-time oxygen measurement in the microenvironment of spheroid cultures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Qi Gao, Shan-Wei Li, Yu-Jie Xie, Min-Xue Zheng, Jing Wei, Zhi-Jun Luo, Xiang-Tong Zhou, Zhi-Gang Liu, Yan Li, Zhi-Ren Wu
Summary: This study proposed the optimal preparation conditions and cultivation equipment of Ca-alginate cell beads for the culturing of anammox sludge. The prepared cell beads had regular spherical shapes, a uniform size, and hard texture. The anammox bacteria in the Ca-alginate cell beads exhibited fast growth and high activity due to diffusion limitations. The gel entrapment method was found to rapidly enrich anammox bacteria in a short period.
WATER SCIENCE AND TECHNOLOGY
(2022)
Article
Optics
Xiangyun LI, Xinlu Zhu, Lin Liu, Fei Wang, Yangjian Cai, Yahong Chen
Summary: We investigate the polarization properties of a tightly focused partially coherent vector beam with a lattice-like initial spatial coherence structure. By analyzing the degree of polarization and polarimetric dimension, we find that this initial coherence structure leads to a 3D isotropically unpolarized beam lattice in the focal plane. Along the longitudinal direction, we observe the formation of nearly 3D unpolarized channels spanning 16 wavelengths near the focal region. We demonstrate that the spatial distribution of the 3D unpolarized lattice can be conveniently controlled by engineering the spatial coherence structure of the incident beam.
Article
Nanoscience & Nanotechnology
Lianyu Lu, Huimin Zhang, Yang Wang, Peng Zhang, Zhi Zhu, Chaoyong Yang
Summary: The newly developed DEL-DMF immunoassay greatly improves the sensitivity and automation of protein bioassays. It utilizes a microfluidics platform and dissolution-enhanced luminescence to control sample transport, immunoreaction, and buffer washing, reducing sample consumption and enhancing binding efficiency. The use of NaEuF4 nanoprobes boosts fluorescence and increases the detection sensitivity. Overall, this novel immunoassay microsystem holds great potential in clinical and biological applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biophysics
Qian Lai, Juan Song, Jie Zha, Huijian Zheng, Manman Deng, Yilong Liu, Wei Lin, Zhi Zhu, Huimin Zhang, Bing Xu, Chaoyong Yang
Summary: The article introduces a novel non-invasive method for monitoring remission status and predicting prognosis in AML patients using the LSC-Chip. By capturing and releasing leukemic stem cells (LSCs), accurate counting of LSCs, subtype classification, and single-cell RNA sequencing analysis can be performed.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Dongxia Chen, Junnan Guo, Ao Li, Chengjie Sun, Huibin Lin, Hongyu Lin, Chaoyong Yang, Wei Wang, Jinhao Gao
Summary: In this study, a metabolic fluorine labeling (MEFLA)-based strategy using 19F MRI is proposed for monitoring the dynamic gut microbiota. Different microbiota subgroups are labeled in situ using a panel of peptidoglycan-targeting MEFLA probes with 19F atoms of different chemical shifts. Real-time in vivo imaging is then achieved using multiplexed hotspot 19F MRI. The strategy allows for extended visualization and semiquantitative analysis of the metabolic dynamics of gut microbiota located at different intestinal sections.
Article
Chemistry, Multidisciplinary
Xiyuan Yu, Wei Lin, Qian Lai, Juan Song, Yilong Liu, Rui Su, Qi Niu, Liu Yang, Chaoyong Yang, Huimin Zhang, Zhi Zhu
Summary: In this study, an Armor strategy was proposed to protect mammalian cell integrity from environmental changes. The Armor was created through dual-aptamer-based hybridization chain reaction (HCR) and Ca2+ assisted calcification for selective cell protection.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Kun Yin, Shanqing Huang, Weidong Ruan, Haopai Wei, He Dong, Dianyi Liang, Meijuan Zhao, Wei Qi, Chaoyong Yang, Zhi Zhu
Summary: Iso-seq is a rapid and uniform single-cell whole-genome sequencing method that uses a specially designed dual-well structure and a capillary-based centrifugal-driven droplet generator to isolate single cells and compartmentalize their genomes in droplets within 3 minutes. Iso-seq achieves high mapping ratio, broad coverage of the genome, and low coefficient of variation, making it promising for a wide variety of biological applications.
Article
Multidisciplinary Sciences
Xiyuan Yu, Weidong Ruan, Fanghe Lin, Weizhou Qian, Yuan Zou, Yilong Liu, Rui Su, Qi Niu, Qingyu Ruan, Wei Lin, Zhi Zhu, Huimin Zhang, Chaoyong Yang
Summary: Single-cell copy number variations (CNVs) are dynamic changes in humans that result in differential gene expression and are associated with adaptive traits or diseases. However, accurate profiling of these CNVs at the single-cell resolution is challenging due to bias in single-cell whole-genome amplification (scWGA) and the limitations of current methods. In this study, we developed a unique single-cell genome library preparation method called dd-scCNV Seq that utilizes digital microfluidics to enable digital counting of CNVs. This approach showed improved accuracy and efficiency compared to existing methods, allowing for automated liquid handling, precise single-cell isolation, and low-cost library preparation. dd-scCNV Seq has the potential to accelerate biological discovery by enabling accurate profiling of CNVs at the single-cell level.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Yinzhu Lu, Bingqian Lin, Weizhi Liu, Jialu Zhang, Lin Zhu, Chaoyong Yang, Yanling Song
Summary: A modular platform is developed for sequential isolation of tumor and non-tumor extracellular vesicles (EVs) using dual-aptamer recognition and tandem microchips. This method provides new clues for assessing immune heterogeneity and can be used for EV proteome profiling.
Article
Chemistry, Physical
Linlin Tang, Mengjiao Huang, Mingjiao Zhang, Yufeng Pei, Yan Liu, Yong Wei, Chaoyong Yang, Teng Xie, Dong Zhang, Ruhong Zhou, Yanling Song, Jie Song
Summary: Multivalent interactions enhance the binding efficiency of ligands to target molecules. A direct SELEX strategy using a preorganized DNA framework library was developed to select an antibody-mimicking multivalent aptamer (Amap) for epithelial cell adhesion molecule (EpCAM). Amap exhibits good binding affinity through both aptamer moieties binding to EpCAM simultaneously, and this strategy has the potential to be a new route for de novo evolution of multivalent aptamers.
Article
Chemistry, Multidisciplinary
Feng Wu, Xiaofeng Chen, Shuli Wang, Ruimin Zhou, Chunyan Wang, Lejian Yu, Jing Zheng, Chaoyong Yang, Xu Hou
Summary: Liquid-like interface design strategy enables high-purity and high-efficiency capture of circulating tumor cells by preventing non-specific binding of proteins and white blood cells.
Article
Chemistry, Analytical
Chunpeng Jiao, Wei Duan, Xian Wu, Yanxue Shang, Fangdou Zhang, Maosheng Zhang, Xi Chen, Jingbin Zeng, Chaoyong Yang
Summary: In this study, a simple, rapid, and ultrasensitive dual-mode pathogen detection platform was developed using a multifunctional nanoprobe and capillary ELISA technology. The novel capillaries modified with capture antibodies can be used for on-site sampling and detection, eliminating the dissociation between sampling and detection in traditional ELISA. The Fe3O4@MoS2 nanoprobe, with excellent photothermal and peroxidase-like activity, was used as an enzyme substitute and signal tag to enhance the immunoassay's sensitivity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xi Zeng, Xiaoxu Guo, Shaowei Jiang, Xiaoping Yang, Zhixing Zhong, Siyu Liu, Zhi Zhu, Jia Song, Chaoyong Yang
Summary: This study presents a digital microfluidics-based single-cell reduced representation bisulfite sequencing (digital-scRRBS) technique, which is an automatic, effective, reproducible, and reagent-efficient method for dissecting the single-cell methylome. The digital-scRRBS allows for rapid isolation of single cells and construction of high-quality methylation sequencing libraries. This technology is valuable for distinguishing cell identity and tracking DNA methylation, and holds great significance for studying rare cells and populations with high levels of heterogeneity.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yahong Chen, Mengyu Zhao, Yifan Ouyang, Suhui Zhang, Zhihan Liu, Kexin Wang, Zhaoxuan Zhang, Yingxia Liu, Chaoyong Yang, Wei Sun, Jie Shen, Zhi Zhu
Summary: Structural DNA nanotechnology can be used to program complex designer structures with molecular precision, directing a wide range of materials. In this protocol, carbon nanotubes are used as model semiconductors to build ultra-scaled, high-performance field-effect transistors from micron-scale DNA templates. The approach enables the assembly of CNT arrays with uniform pitches and clean interfaces, displaying superior performance compared to previous examples of biotemplated electronics.
Article
Chemistry, Physical
Xi Zeng, Shaowei Jiang, Zhixing Zhong, Xiaoping Yang, Qiuyue Chen, Jin Li, Zhi Zhu, Jia Song, Chaoyong Yang
Summary: DIRECT is a digital microfluidics-based method that allows for simultaneous analysis of DNA methylation and gene expression in a single library construction, providing a cost-effective and simplified approach for single-cell analysis.
Article
Chemistry, Multidisciplinary
Guihua Zhang, Yaohui Wang, Weihang Zhou, Yanmei Lei, Jinsong Lu, Wenjin Yin, Zhi Zhu, Chaoyong Yang, Peng Zhang
Summary: A magnetically driven tandem chip integrated with magnetic nanomixers and cyclic SERS tags was developed to achieve streamlined rapid isolation and multiplexed profiling of EVs. Six protein markers were analyzed simultaneously using only 10 μL plasma within 1.5 hours. The EV signature offers precise diagnosis of breast cancer patients.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Nanoscience & Nanotechnology
Xing Xu, Qiannan Zhang, Mingyin Li, Shiyan Lin, Shanshan Liang, Linfeng Cai, Huanghuang Zhu, Rui Su, Chaoyong Yang
Summary: Cellular heterogeneity is crucial for biological processes. In recent years, the development of single-cell multiomics technologies has allowed for comprehensive evaluation of single-cell heterogeneity. Microfluidics plays a significant role in single-cell multiomics analysis, enabling improved detection sensitivity, accuracy, throughput, and integration. This review provides an overview of the latest microfluidic single-cell multiomics analysis techniques, as well as their potential in biological and clinical applications.