期刊
ANALYTICAL CHEMISTRY
卷 86, 期 15, 页码 7377-7382出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac5005794
关键词
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资金
- Maryland NanoCenter
- NispLab
- U.S. Army Research Laboratory [NNL09AA00A]
Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 mu g/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.
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