Article
Chemistry, Analytical
Guoliang Zheng, Jiangshan Dai, Haiyan Wang, Ling Li, Deyu Yuan, Shujun Bai, Xu Song, Yongyun Zhao
Summary: HNESA is a novel signal amplification technique for nucleic acid detection which overcomes the limitations of traditional methods, demonstrates high sensitivity, can distinguish mismatched sequences, and has potential applications in complex biological samples.
Article
Chemistry, Analytical
Jiao Yang, Shilan Fu, Fang Luo, Longhua Guo, Bin Qiu, Zhenyu Lin
Summary: A novel homogeneous PEC biosensor based on target-responsive hydrogels has been developed for sensitive detection of miRNA-155. By utilizing a cascade amplification strategy, the biosensor exhibits high sensitivity with a low detection limit of 0.41 fM and a wide linear range from 1.0 fM to 100 pM. The simplicity, reproducibility, and storage stability of the prepared hydrogels make this biosensing platform versatile for diverse target detection.
Article
Chemistry, Analytical
Yu Gu, Xuejiao Yang, Shanwen Hu, Chunxian Guo, Bo Chen, Cunxia Fan, Chang Ming Li
Summary: A novel glucometer-based microfluidic platform was developed for sensitively detecting cancer biomarker HER2. The assay uses rolling cycle amplification technology with a miniaturized personal glucose meter, on a small-sized microfluidic chip with high sensitivity and wide dynamic range, showing great potential for clinical diagnosis at home or in urban areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Linhong Ning, Heng Cheng, Feng Yu, Yuan Zhou, Yang Xie
Summary: Pancreatic diseases, such as pancreatitis and pancreatic cancer, are highly threatening gastrointestinal diseases due to their atypical symptoms. This study developed a sensitive and simple method for detecting exosomal miRNA, using only one dual-hairpin-probe. The method showed high stability and discriminative capability, making it a promising tool for early diagnosis of pancreatic cancers.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qing Tang, Jun Xu, Siqi Wei, Haoyi Chen, Jiapeng Chen, Huilin Zhang, Lihong Liu
Summary: An sensitive and label-free CRP assay was developed using cyclic enzymatic signal amplification and capillary electrophoresis with UV detection, resulting in a wide linear range in 1% human serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Shu-Ying Ye, Chang-Gang Pan, Yu-Hua Dai, Guo-Xi Liang
Summary: An ultrasensitive biosensor for the detection of telomerase activity was constructed utilizing gold@Carbon dots nanohybrids (Au@CDs) as a high efficiency electrochemiluminescence (ECL) emitter and nicking enzyme assisted signal amplification (NESA) technology. By employing a dual signal amplification strategy, the biosensor showed excellent sensitivity and detection limit for telomerase activity.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Yan Liu, Shao-Hua Wu, Xing-Yuan Du, Jian-Jun Sun
Summary: A plasmonic Ag nanocube (AgNC) enhanced SERS biosensing platform was proposed for sensitive detection of DNA related to oral cancer. The combination of SERS tags, high temperature, and Nt.BstNBI-assisted target recycling amplification contributed to the high sensitivity of the biosensor.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Hyo Yong Kim, Jayeon Song, Hyun Gyu Park
Summary: An ultrasensitive isothermal method for detecting microRNA using target-induced chain amplification reaction (CAR) is described. The strategy involves a hairpin probe (HP) that opens upon binding to target miRNA, leading to exponential amplification reactions and the production of double-stranded DNA products that can be monitored through fluorescent signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Monisha Elumalai, Andrey Ipatov, Joana Carvalho, Joana Guerreiro, Marta Prado
Summary: A new DNA target amplification strategy combined with dual-mode colorimetric naked eye and smartphone analysis is proposed for the rapid and sensitive detection of zebra mussel environmental DNA. This method demonstrates high specificity and sensitivity, suitable for field analysis of environmental samples without the need for expensive laboratory equipment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Changjing Yuan, Jie Fang, Xizi Luo, Yang Zhang, Guorong Huang, Xiaojun Zeng, Ke Xia, Mengya Li, Xueping Chen, Xiang Yang, Marc Lamy de la Chapelle, Weiling Fu
Summary: Early diagnosis of cancers such as pancreatic cancer, which has atypical symptoms and high mortality, is crucial. Exponential amplification reaction (EXPAR) is an effective isothermal amplification method for detecting microRNAs (miRNAs), but it lacks specificity. To address this issue, a nicking-assisted entropy-driven DNA circuit triggered exponential amplification reaction (NAED-EXPAR) is proposed.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Can Peng, Fan Wu, Youhui Zeng, Bo Liu, Ruiying Peng, Jing Zheng
Summary: In this study, we developed an azoreductase activatable, endonuclease-gated fluorescent nanodevice for amplification imaging of microRNA-21 in hypoxic tumor cells. Our work provides a new tool for accurate monitoring of intracellular biomolecule abundance and disease diagnosis in the future.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Zhaohui Chu, Jingyi Chen, Jingzi Zhang, Qihui Xie, Fan Zhang, Qingjiang Wang
Summary: Efficient detection of pathogenic bacteria is crucial for food safety and preventing foodborne illnesses. In this study, a capillary electrophoresis coupled laser-induced fluorescence assay was developed for the detection of Escherichia coli by detecting its specific DNA. The method showed high sensitivity and was successfully applied to the detection of commercially available skim milk samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Multidisciplinary Sciences
Sijia Chen, Yuhan He, Lin Liu, Jianxiu Wang, Xinyao Yi
Summary: Abnormal expression of miRNA is associated with tumor diseases, and a SPR assay for miRNA-182 can facilitate early diagnosis of glioma.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Bin Zhou, Lei Lin, Bingzhi Li
Summary: The study developed a fluorescent biosensor based on EXPAR for rapid and sensitive detection of FEN1, showing good selectivity and high amplification efficiency. This biosensor holds promise for FEN1-related biomedical investigations.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Chun-Yi Wu, Yi-Ting Su, Cheng-Kuan Su
Summary: We successfully fabricated an all-in-one needle panel meter for the on-site determination of urea and glucose using four-dimensional printing (4DP) and digital light processing three-dimensional printing (3DP) technologies. By measuring the bending of the needle caused by pH changes and coupling it with specific reactions, we were able to quantitatively analyze urea and glucose. The method's detection limits were 4.9 and 7.0 μM, and it can be used within a concentration range of 0.1 to 10 mM.
BIOSENSORS & BIOELECTRONICS
(2023)