Article
Chemistry, Analytical
Michael R. Armbruster, Mahmoud Elhusseiny Mostafa, Rhea N. Caldwell, Scott F. Grady, Christopher K. Arnatt, James L. Edwards
Summary: Two derivatization strategies are combined to separate and quantify saturated and monounsaturated fatty acids, with further differentiation of double bond isomers using fragmentation. This method is high-throughput and accurate, revealing important biological changes.
Article
Biochemistry & Molecular Biology
Jing Zhao, Mengxuan Fang, Yu Xia
Summary: This study developed a workflow for global quantitation of fatty acids, including C=C location isomers, via charge-tagging Patern`o-Buchi (PB) derivatization and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method enabled deep profiling of unsaturated fatty acids in yak milk powder and pooled human plasma, achieving successful quantitation across a wide range of concentrations.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Hua Zhang, Meng Xu, Xudong Shi, Yuan Liu, Zihui Li, Justin C. Jagodinsky, Min Ma, Nathan Welham, Zachary S. Morris, Lingjun Li
Summary: This study presents a novel method using PAA-induced epoxidation combined with mass spectrometry to localize the C=C bond in unsaturated fatty acids, allowing for quantification and spatial visualization of FA isomers in biological samples.
Article
Chemistry, Analytical
Bo Zhang, Yunjun Wang, Bo-Wen Zhou, Jie Cheng, Qi Xu, Li Zhang, Tuan-qi Sun, Jing Zhang, Yin-long Guo
Summary: Specific locations of carbon-carbon double bonds (C=C) in lipids play important roles in biological processes. A N-tosylaziridination method was developed to annotate C=C positions in lipids using mass spectrometry, and it showed high efficiency, sensitivity, and specificity. Studies on human thyroid tissues suggested that increased unsaturated fatty acid content and n-6/n-3 ratios were closely associated with thyroid tumorigenesis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xiuting Chen, Zhixin Xiong, Meixian Yang, Yu Gong
Summary: A fast and reliable mass spectrometry-based method has been developed to discriminate between the positional isomers of o-, m-, and p-C6H4XCO2H. The method relies on the distinct fragmentation patterns of isomeric ThCl4(C6H4XCO2)(-) ions generated by electrospray ionization, and allows for convenient quantification of the positional isomers without any pre-treatment.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Hanlin Ren, Alexander Triebl, Sneha Muralidharan, Markus R. Wenk, Yu Xia, Federico Torta
Summary: Lipids are crucial biomolecules in living systems and can be used as biomarkers and drug targets in metabolic diseases. Through the use of modern mass spectrometry, researchers have been able to study the diverse distribution of C=C locations in lipid isomers across tissues. Mapping the distribution of isomers in different mouse tissues has provided valuable insights into lipid metabolism pathways.
Article
Chemistry, Analytical
Kimberly C. Fabijanczuk, Hsi-Chun Chao, Joshua L. Fischer, Scott A. McLuckey
Summary: Phosphoinositides, phosphorylated derivatives of phosphatidylinositols, are crucial signaling phospholipids in mammalian cellular membranes. Identifying and characterizing these phosphoinositides and their isomers accurately using traditional shotgun mass spectrometry is challenging. However, utilizing novel gas-phase ion/ion chemistry expands the range of precursor ion-types and allows for structural characterization of phosphoinositides. This method provides valuable information on the phosphorylation degree, fatty acyl composition, and isomeric species.
Article
Chemistry, Analytical
Samuel W. J. Shields, James D. Sanders, Jennifer S. Brodbelt
Summary: Understanding the structures and functions of lipids is important in lipidomics research. This study introduces a technique called precursor exclusion (PEx) UVPD, which improves the signal-to-noise ratio of low-abundance fragment ions obtained from UVPD of glycerophospholipids. The method allows for deeper structural analysis of lipids and confident identification of different lipid structures.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Anqi Tu, Kenneth P. Garrard, Neveen Said, David C. Muddiman
Summary: The use of mCPBA for on-tissue epoxidation in MS imaging allows for rapid and simple identification and relative quantification of double-bond positional isomers, with high efficiency and sensitivity. Tandem MS analysis of the epoxidized products generated informative fragment ions specific to the double-bond positions, enabling relative quantification of positional isomers in binary mixtures across a wide mole fraction range. The innovative spiral scan pattern during data acquisition provides insights into the major isomeric compositions of multiple UFAs from a tissue section in a single run.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Multidisciplinary
Huanhuan Yao, Tai Wu, Cheng Wu, Liming Ding, Yong Hua, Feng Hao
Summary: This study aims to improve the exciton binding energy and film quality of 2D Dion-Jacobson (DJ) tin halide perovskite by designing organic spacers. The results show that ortho-phenylenediamine (o-PDA) spacer reduces the exciton binding energy and enhances structural stability, resulting in dense and uniform high-quality films. Furthermore, o-PDA spacer enables effective charge transfer and achieves a record power conversion efficiency of 7.18% for 2D DJ (o-PDA)FA3Sn4I13 solar cells.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Xianlin Han, Richard W. Gross
Summary: This article introduces the foundations, advances, and future directions of the lipidomics field. Technological breakthroughs in the 1980s laid the groundwork for lipidomics and subsequent innovations in fragmentation, resolution, and sample analysis drove its rapid growth. Strategic approaches, such as direct infusion and chromatographic separation, facilitated the study of low abundance lipid species.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Carla Ferreri, Alessandra Ferocino, Gessica Batani, Chryssostomos Chatgilialoglu, Vanda Randi, Maria Vittoria Riontino, Fabrizio Vetica, Anna Sansone
Summary: Plasmalogens are membrane phospholipids that play a role in peroxidation and have the potential for cis-trans double bond isomerization. Through experiments using representative molecules and liposomes under specific conditions, the reactivity and outcomes of these processes were studied. The results provide important insights for lipidomic applications and understanding radical stress in living organisms.
Article
Chemistry, Analytical
Julia R. Bonney, Woo-Young Kang, Jonathan T. Specker, Zhongling Liang, Troy R. Scoggins, Boone M. Prentice
Summary: This study combines infrared multiphoton dissociation with a mass spectrometer to rapidly fragment ion/ion reaction products in imaging mass spectrometry experiments. The ion/ion reaction images allow for the mapping of the relative spatial distribution of multiple lipid isomers, highlighting the importance of resolving isomers in imaging mass spectrometry experiments.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hai-Fang Li, Jing Zhao, Wenbo Cao, Wenpeng Zhang, Yu Xia, Zheng Ouyang
Summary: This study developed a novel approach to improve the efficiency and sensitivity of ultraviolet photodissociation tandem mass spectrometry (UVPD MS/MS) for structural determination of lipids by incorporating a chromophore to the C=C moiety in fatty acyls. The results demonstrated the effectiveness of this approach in identifying the C=C bond locations in unsaturated lipids from complex lipid extracts.
Article
Chemistry, Physical
Akshay Vyas, Rohit R. Koshti, H. N. Patel, Chetan B. Sangani, A. K. Prajapati, Yongfang Yao, Yong-Tao Duan
Summary: The effect of position isomer in newly synthesized liquid crystalline compounds on their mesophase behavior was studied. The synthesized compounds were characterized using spectroscopic techniques, and their phase transition behavior was observed. The interaction between molecules was investigated and compared with other compounds.
JOURNAL OF MOLECULAR LIQUIDS
(2022)