Article
Biochemical Research Methods
Kendall R. Johnson, Michal Gregus, Alexander R. Ivanov
Summary: In this study, the combination of high-field asymmetric waveform ion mobility spectrometry (FAIMS) with ultrasensitive capillary electrophoresis hyphenated with tandem mass spectrometry (CE-MS/MS) was used for deep proteome coverage of low nanogram amounts of digested cell lysates. The optimized CE-FAIMS-MS/MS method showed significant improvements in protein and peptide identification, as well as detection of multiphosphorylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Daoyang Chen, Zhichang Yang, Xiaojing Shen, Liangliang Sun
Summary: Top-down proteomics (TDP) is an ideal approach for deciphering the histone code and requires new analytical tools with high-capacity separation and highly sensitive detection of proteoforms. In this study, capillary zone electrophoresis (CZE) -MS/MS was used to identify a large number of histone proteoforms with higher sensitivity compared to traditional methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Mary Angela Favaro Perez, Daniela Daniel, Marisa Padula, Claudimir Lucio do Lago, Carla Beatriz Grespan Bottoli
Summary: This study developed an efficient method for the rapid detection of primary aromatic amines in cooking utensils, revealing that some of the samples did not meet the standards.
Article
Biochemical Research Methods
Mark Ivanov, Julia A. Bubis, Vladimir Gorshkov, Daniil A. Abdrakhimov, Frank Kjeldsen, Mikhail Gorshkov
Summary: The study introduced a new fast proteomic method, DirectMS1, which, combined with machine-learning algorithm and other tools, successfully identified a large number of proteins efficiently in a short period of time.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Yusuke Kawashima, Hirotaka Nagai, Ryo Konno, Masaki Ishikawa, Daisuke Nakajima, Hironori Sato, Ren Nakamura, Tomoyuki Furuyashiki, Osamu Ohara
Summary: This paper discusses the demand for high proteome coverage in single-shot measurements and focuses on data-independent acquisition (DIA)-MS and ion mobility spectrometry as techniques for deep proteome analysis. The researchers aimed to expand the proteome coverage by optimizing high-field asymmetric waveform ion mobility spectrometry parameters in DIA-MS. The approach was applied to the analysis of host proteins in mouse feces and proved to be useful in understanding mental illness pathologies.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Chemistry, Analytical
Yuanyuan Wu, Wenmei Zhang, Yaoyao Zhao, Xiayan Wang, Guangsheng Guo
Summary: Research on single-cell proteomics is crucial for understanding the growth, development, and diseases in organisms. However, the complexity and limited quantity of proteins in single cells have hindered the progress in this field. Mass spectrometry-based single-cell proteomics, particularly electrospray ionization mass spectrometry (ESI-MS), has rapidly advanced due to its minimal sample requirements, high sensitivity, and structural information without antibody labeling. Nevertheless, the variations in pretreatment methods, separation techniques, and MS instruments greatly affect the throughput and coverage of single-cell proteomic technologies. This review categorizes recent publications based on different methods for single-cell protein sample separation, highlights the performance of these methods in terms of detection coverage and throughput, and discusses the applications and future prospects of ESI-MS-based single-cell proteomics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Jasmine S. Furter, Peter C. Hauser
Summary: This study utilizes a low-cost microfluidic breadboard approach with pneumatically actuated pumps and valves for efficient analysis, successfully detecting benzalkonium ions and four pesticides.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Radim Polasek, Klara Konderlova, Jan Petr
Summary: The aim of this study was to develop a new method for analyzing tamsulosin enantiomers using capillary electrophoresis combined with tandem mass spectrometry. The method achieved a limit of detection of 1.6 nmol L-1 under optimal conditions. It was validated for linearity, detection and quantification limits, precision, recovery, and selectivity, and can be applied to the analysis of tamsulosin enantiomers in environmental samples and other analytical tasks.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This review provides an overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in proteomics and peptidomics. It covers topics such as sample preparation, hyphenation with mass spectrometry, and multidimensional separations. New developments in both bottom-up and top-down approaches in proteomics are discussed, along with various applications in protein posttranslational modifications, biological and biochemical research, clinical peptidomics and proteomics, and food analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Behrouz Seyfinejad, Abolghasem Jouyban
Summary: Accurate and reliable results are crucial in pharmaceutical and biomedical analyses. The combination of mass spectrometry and capillary electrophoresis shows great potential in these fields. This review provides an update on recent applications and methods of CE-MS relevant to biomedical and biopharmaceuticals, and discusses the latest developments in CE-MS and different CE modes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Emmanouil Mavrogeorgis, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, Joachim Jankowski
Summary: In recent years, CE-MS has been increasingly used in clinical research for chronic and age-associated diseases, allowing for comprehensive assessment of small molecular weight proteins and peptides. The study evaluated the analytical performance of CE-MS in terms of reproducibility, variability, and efficiency in peptide detection, showing high correlation among different runs and low variation in signal intensity. These findings support the value of CE-MS for clinical application.
Article
Chemistry, Analytical
Cedric Delvaux, Marjorie Dauvin, Madeleine Boulanger, Loic Quinton, Dominique Mengin-Lecreulx, Bernard Joris, Edwin De Pauw, Johann Far
Summary: Peptidoglycan (PGN) is a crucial structure in bacterial cell walls, continuously synthesized and degraded during bacterial life cycle for growth and division. PGN fragments act as messenger molecules in bacterial cell wall stress response and triggering mammalian innate immune response. Bacteria modulate their PGN degradation or recognition by chemically modifying PGN, such as amidating the epsilon-carboxyl group of meso-diaminopimelic acid in the PGN peptide chain. The quantitation of PGN-derived peptides is challenging due to their hydrophilic nature, but capillary zone electrophoresis coupled with high-resolution mass spectrometry shows excellent repeatability in quantitation of PGN peptides and their derivatives in bacterial cytoplasmic extracts.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Bryon S. Drown, Kevin Jooss, Rafael D. Melani, Cameron Lloyd-Jones, Jeannie M. Camarillo, Neil L. Kelleher
Summary: To understand the human body's functions, it is necessary to study the relationship between protein structure and function on a large scale. Proteins' chemical structure is regulated at multiple levels, resulting in various products with different functions within cells. The term "proteoform" encompasses the complexity of chemical composition. Mapping the proteoform landscape in human tissues requires sensitive and comprehensive analytical techniques. In this study, a top-down proteomics approach combining capillary zone electrophoresis and nanoflow reversed-phase liquid chromatography was used to identify and characterize proteoforms from different organs. The combination of these techniques provided enhanced coverage of post-translational modifications and proteoforms. Among the identified proteoforms, a significant percentage had not been reported before. The differential regulation of proteoforms in different organs provides insights into their function and regulation in human health and disease.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Qianyi Wang, Fei Fang, Qianjie Wang, Liangliang Sun
Summary: The characterization of histone proteoforms with various post-translational modifications is crucial for understanding their role in epigenetic control of gene expression. This study presents a novel approach combining capillary zone electrophoresis, ion mobility spectrometry, and mass spectrometry for online multi-dimensional separations of histone proteoforms. The results demonstrate that this technique can greatly improve the identification of histone proteoforms and provide a high sensitivity tool for their comprehensive characterization.
