Article
Chemistry, Analytical
Korakot Boonyaphon, Zhenglin Li, Sung-Jin Kim
Summary: In this study, a microfluidic recirculation system driven by water-head pressures was developed, which enables media recirculation in parallel channels with pulsatile and constant flows. The system allows control of flow rates and shear stress, and can be used for systematic and parallel analysis of cell response to fluidic shearing patterns.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Zhenglin Li, Bongjun Yeom, Sung-Jin Kim
Summary: This study introduced a microfluidic platform for fast and parallel Layer-by-Layer assembly, demonstrating the significant impact of shear stress on the thickness and transmittance of coated films. The platform allows for efficient determination of optimized coating conditions, with coating duration 15 times faster than conventional methods.
Article
Chemistry, Multidisciplinary
Thomas Gervais, Yuksel Temiz, Lucas Aube, Emmanuel Delamarche
Summary: This study reports a capillary microfluidic architecture based on self-coalescence modules, which can store a large number of reagents in small aliquots and reconstitute them independently using a simple pipetting step. A mathematical model is provided to guide the spotting of reagents and enable the generation of complex multi-reagent chemical patterns. The results demonstrate the accurate and versatile formation of chemical patterns, as well as simple methods for integrating reagents and imaging the resulting patterns.
Article
Chemistry, Analytical
Shan He, Huiting Lian, Xuegong Cao, Bin Liu, Xiaofeng Wei
Summary: A light-driven photocatalytic-photothermal synergetic system integrated with a microfluidic distance-based analytical device (PCPT-mu DAD) was developed for visual, portable, sensitive, and quantitative detection of targets. This system effectively translates target concentration into a visual distance signal readout by utilizing the photosensitization and photothermal effect.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Razieh Salahandish, Mohsen Hassani, Azam Zare, Fatemeh Haghayegh, Amir Sanati-Nezhad
Summary: The study explores the integration of electrochemical biosensors into fluid handling units to develop point-of-care platforms for quantitative detection of bodily fluid markers. However, current device designs lack full assay automation and require manual loading of reagents or external devices for liquid manipulation. The proposed microfluidic platform addresses challenges by automating steps of electrochemical biosensing assays and optimizing design components for high reproducibility and minimal noise-induced to the biosensor.
Article
Physics, Multidisciplinary
Sameh Kaziz, Yosra Saad, Mohamed Hichem Gazzah, Hafedh Belmabrouk
Summary: In this study, 3D finite element simulations were conducted to investigate the binding reaction kinetics of SARS-CoV-2 S protein and its immobilized antibody in a microfluidic immunoassay. The application of ACET force was found to enhance the binding efficiency by reducing the diffusion boundary layer and shortening the detection time of the biosensor.
EUROPEAN PHYSICAL JOURNAL PLUS
(2022)
Article
Environmental Sciences
Lulu Zheng, Mantong Zhao, Bo Dai, Zhiwei Xue, Yi Kang, Sixiu Liu, Lianping Hou, Songlin Zhuang, Dawei Zhang
Summary: This article introduces an integrated system for the enrichment and detection of polycyclic aromatic hydrocarbons (PAHs) in the air. The system consists of channels with threaded structures and curved channels, which have high capture efficiency for PAHs. The PAHs-carried particles can be crushed into the detection chip for testing. The whole process takes about 25 minutes, with the advantages of low cost, low reagent consumption, simple operation, semi-automatic operation, high sensitivity, high speed, and high throughput, showing the potential to become an air pollution monitoring platform.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Yupan Wu, Bowen Hu, Xun Ma, Yucheng Wang, Wei Li, Shaoxi Wang
Summary: In this study, a microfluidic immunosensor with a sinusoidal bipolar electrode is proposed to enhance the rate of antibody binding and improve the detection speed. The results demonstrate the potential of this approach for various immunoassay applications.
Article
Multidisciplinary Sciences
Bowei Li, Ji Qi, Feng Liu, Rongfang Zhao, Maryam Arabi, Abbas Ostovan, Jinming Song, Xiaoyan Wang, Zhiyang Zhang, Lingxin Chen
Summary: This article introduces an indirect fluorescence sensing mechanism using the charge transfer effect and smart molecular imprinting technology for the detection of non-fluorescent substances. By immobilizing a molecular imprinted thin film on the surface of nanoparticles and implementing the mechanism on a paper-based microfluidic chip, trace detection of microcystin is achieved.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Xinran Xiang, Qinghua Ye, Yuting Shang, Fan Li, Baoqing Zhou, Yanna Shao, Chufang Wang, Jumei Zhang, Liang Xue, Moutong Chen, Yu Ding, Qingping Wu
Summary: A novel microfluidic chip was developed for rapid and sensitive detection of AFB1, showing a wide analytical range and low detection limit, as well as good specificity and reusability. The chip design provides a reliable scheme for on-site detection of AFB1 and analysis of other small molecules.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Computer Science, Hardware & Architecture
Victor Malyshkin, Darkhan Akhmed-Zaki, Vladislav Perepelkin
Summary: The LuNA system addresses the issue of efficient parallel execution of numerical algorithms for supercomputers by representing application algorithms in a hardware-independent high-level form and automatically constructing various parallel programs with different non-functional properties. The efficiency problem of automatically constructed parallel programs is addressed through the behavior concept, allowing control of parallel program behavior without low-level programming.
JOURNAL OF SUPERCOMPUTING
(2021)
Review
Biochemical Research Methods
Ines F. Pinto, Saara Mikkonen, Leila Josefsson, Meeri E-L Makinen, Ruben R. G. Soares, Aman Russom, Asa Emmer, Veronique Chotteau
Summary: This review discusses the importance of deep knowledge and control of a larger panel of culture components in mammalian cell cultures for process optimization, control, and robustness. It highlights key advances in microfluidic affinity assays and microchip capillary electrophoresis for the miniaturization and integration of Process Analytical Technology. The article also introduces a comprehensive toolbox of discrete analytical components that can potentially support the translation of mature microfluidic technologies towards novel applications.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Tianyi Liu, Zhiming Liu, Fanglai Zhu, Zhiyang Mao
Summary: This article proposes a method to build a dual parallel PMSM based on traditional PMSM control hardware without additional sensors, and verifies the performance of the system and the robustness of the controller through experiments.
IEEE TRANSACTIONS ON POWER ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Gokul Chandra Biswas, Hiroaki Suzuki
Summary: We have introduced a simple-to-use manual roller pump-driven and valve-free microfluidic system for sequential solution exchange and protein detection. The system is characterized by its simplicity, quick operation, and suitability for emergencies and situations with limited resources.
Review
Biotechnology & Applied Microbiology
Jinfeng Yan, Tong Wu, Jinjin Zhang, Yueyue Gao, Jia-Min Wu, Shixuan Wang
Summary: A comprehensive understanding of the female reproductive system is crucial for protecting fertility and preventing women's health issues. Microfluidic chips have revolutionized the knowledge and management of female reproductive health, ultimately promoting the development of assisted reproductive technologies, treatments, and drug screening approaches.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Hematology
Shannon R. McCurdy, Vedran Radojcic, Hua-Ling Tsai, Ante Vulic, Elizabeth Thompson, Sanja Ivcevic, Christopher G. Kanakry, Jonathan D. Powell, Brian Lohman, Djamilatou Adom, Sophie Paczesny, Kenneth R. Cooke, Richard J. Jones, Ravi Varadhan, Heather J. Symons, Leo Luznik
Summary: This study used machine learning to analyze the immunologic signatures associated with posttransplant outcomes in bone marrow transplantation using PTCy. The results identified specific lymphocyte subsets that predict clinical outcomes and offered new insights into the influence of PTCy on alloimmune responses. The study highlights the importance of NK cell counts and CD4(+) regulatory T cells in patient survival and acute graft-versus-host disease. The findings contribute to the discovery of biomarkers in bone marrow transplantation and provide directions for future therapeutic interventions.
Article
Chemistry, Multidisciplinary
Islam Seder, Ahla Jo, Bong-Hyun Jun, Sung-Jin Kim
Summary: In this study, a microfluidic platform is presented for the simple, automated, and highly sensitive immunoassay of viral antigens. By utilizing the motion of the layers and the surface tension in the liquids, reagents are able to mix homogeneously between the top and bottom chambers. The automated device achieves a highly sensitive magnetic bead-based sandwich immunoassay for the influenza A H1N1 virus within 32.5 min using magnetic beads and quantum dots.
Article
Engineering, Chemical
Thi Hong Tham Phan, Sung-Jin Kim
Summary: In this study, a simple, fast, and inexpensive method for fabricating super-hydrophobic microchannels was presented. The microchannels exhibited a micro-textured surface with a high water contact angle, allowing for the generation of high-speed and size uniform droplets. This method has potential applications in gelation and fluid control.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Chemistry, Analytical
Phi Hung Hoang, Islam Seder, Ana Isabel Ferrer Ramirez, Sung-Jin Kim
Summary: This study presents a fully automated and effective fluidic system for the isolation and detection of cfDNA from whole blood. The system utilizes disposable chambers and key components such as the sedimentation chamber and plunger chamber to achieve plasma separation and cfDNA capture, followed by detection using real-time PCR on a chip.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Korakot Boonyaphon, Zhenglin Li, Sung-Jin Kim
Summary: In this study, a microfluidic recirculation system driven by water-head pressures was developed, which enables media recirculation in parallel channels with pulsatile and constant flows. The system allows control of flow rates and shear stress, and can be used for systematic and parallel analysis of cell response to fluidic shearing patterns.
ANALYTICA CHIMICA ACTA
(2022)
Article
Medicine, Research & Experimental
Baskar Ramdas, Palam Lakshmi Reddy, Raghuveer Singh Mali, Santhosh Kumar Pasupuleti, Ji Zhang, Mark R. Kelley, Sophie Paczesny, Chi Zhang, Reuben Kapur
Summary: Heterozygous mutations in FLT3(ITD), TET2, and DNMT3A are associated with hematologic malignancies in humans. This study investigated the co-occurrence of these mutations in a mouse model of acute myeloid leukemia (AML) and found that mice with all three mutations showed an aggressive form of AML. The gene expression pattern in these mice was similar to the human TFD gene signature at the single-cell RNA level. Importantly, the TFD-driven AML responded to a combination of drugs in both mouse and patient-derived models.
Meeting Abstract
Hematology
Chao-Yie Yang, Xinrui Yuan, Hua Jiang, Jason C. Rech, Denggang Fu, Aaron Robida, Krishani Rajanayake, Hebao Yuan, Bo Wen, Duxin Sun, Krishnapriya Chinnaswamy, Jeanne A. Stuckey, Sophie Paczesny
Meeting Abstract
Hematology
Denggang Fu, Hua Jiang, Sophie Paczesny
Article
Oncology
Pietro Crivello, Esteban Arrieta-Bolanos, Meilun He, Tao Wang, Stephanie Fingerson, Shahinaz M. Gadalla, Sophie Paczesny, Steven G. E. Marsh, Stephanie L. J. Lee, Stephen R. Spellman, Yung-Tsi Bolon, Katharina Fleischhauer
Summary: This study found that immunopeptidome divergence is a determinant of T-cell alloreactivity and clinical tolerability in single class I HLA-mismatched unrelated donor hematopoietic cell transplantation. Using hierarchical clustering of peptide-binding motifs, the authors classified 68.1% of the HLA-mismatched transplants as matched or mismatched. Mismatched transplants were associated with lower overall survival.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Biochemical Research Methods
Md. Emamul Kabir, Sung-Jin Kim
Summary: We propose a novel method for fabricating round cross-sectional channels for autoregulatory pressure regulators. By using a thermal expansion technique, we can create round channels in replica layers using air in the cavity space of a master mold. Our method allows for adjustable width and height of the channels, ranging from 80-300 μm and 3-57 μm, respectively. Compared to devices with rectangular channels, our pressure regulator with a round channel achieves constant output pressure at a lower threshold input pressure of less than 77%.
Review
Hematology
Alan Bidgoli, Brittany Paige DePriest, Maedeh Vakili Saatloo, Hua Jiang, Denggang Fu, Sophie Paczesny
Summary: Hematopoietic cell transplantation is a potentially curative treatment for hematologic and nonhematologic disorders. Graft-versus-host disease is a major complication post-transplantation, and biomarkers play a significant role in disease diagnosis and management.
TRANSPLANTATION AND CELLULAR THERAPY
(2022)
Article
Biochemical Research Methods
Islam Seder, Hyomin Moon, Su Jin Kang, Sehyun Shin, Won Jong Rhee, Sung-Jin Kim
Summary: This study introduces a microfluidic device that can isolate extracellular vesicles (EVs) with multiple size intervals in a simple and automated manner. By adjusting plunger speed, using a vertically movable plunger, and rotationally movable chip, high separation resolution and high-purity EVs were obtained. The device has potential applications in molecular analysis and EV-based biomarker discovery using various biofluids.
Meeting Abstract
Cell Biology
Johannes Schetelig, Henning Baldauf, Falk Heidenreich, Jorinde D. Hoogenboom, Stephen Spellman, Aleksandr Kulagin, Thomas Schroeder, Henrik Sengeloev, Peter Dreger, Edouard Forcade, Jan Vydra, Eva-Maria Wagner-Drouet, Goda Choi, Shankara Paneesha, Nuno Miranda, Alina Tanase, Liesbeth C. de Wreede, Vinzenz Lange, Alexander H. Schmidt, Juergen Sauter, Stephanie Lee, Yung-Tsi Bolon, Meilun He, Steven G. E. Marsh, Shahinaz Gadalla, Sophie Paczesny, Annalisa Ruggeri, Christian Chabannon, Katharina Fleischhauer
Meeting Abstract
Cell Biology
Pietro Crivello, Esteban Arrieta-Bolanos, Meilun He, Tao Wang, Shahinaz M. Gadalla, Sophie Paczesny, Steven G. E. Marsh, Stephanie J. Lee, Stephen R. Spellman, Yung-Tsi Bolon, Katharina Fleischhauer
Meeting Abstract
Cell Biology
Esteban Arrieta-Bolanos, Pietro Crivello, Meilun He, Tao Wang, Shahinaz Gadalla, Sophie Paczesny, Steven G. E. Marsh, Stephanie J. Lee, Stephen R. Spellman, Yung-Tsi Bolon, Katharina Fleischhauer