期刊
ANALYTICAL CHEMISTRY
卷 85, 期 20, 页码 9671-9677出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac401985r
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资金
- NIH NCI [T32CA138312]
- Department of Defense CDMRP Program [BC100510]
- NIH [R21CA147831]
- Life Science Discovery Fund
- CDMRP [545243, BC100510] Funding Source: Federal RePORTER
Ensemble-decision aliquot ranking (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. Here, we report the next generation of eDAR, where we designed and optimized a new hydrodynamic switching scheme for the active sorting step in eDAR, which provided fast cell sorting with an improved reproducibility and stability. The microfluidic chip was also simplified by incorporating a functional area for subsequent purification using microslits fabricated by standard lithography method. Using the reported second generation of eDAR, we were able to analyze 1 mL of whole-blood samples in 12.5 min, with a 95% recovery and a zero false positive rate (n = 15).
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