期刊
ANALYTICAL CHEMISTRY
卷 85, 期 10, 页码 4925-4929出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac3034618
关键词
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资金
- SCF (Special Coordination Funds for Promoting Science and Technology) of MEXT
- Japan Society [24685013]
- Japan Science and Technology Agency for Adaptable and Seamless Technology Transfer Program
- Grants-in-Aid for Scientific Research [25288039, 25288069, 24685013] Funding Source: KAKEN
Specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface of overoxidized polypyrrole (OPPy) film. The recognition of target bacteria was successfully carried out in real time using OPPy film in combination with dielectrophoresis. The unique combination of both techniques made the specific detection of template bacilli possible at concentrations as low as 10(3) CFU/mL within 3 min, without any bacterial pretreatment. The observation of the movement of bacteria by using a fluorescent microscope revealed that living bacteria were being trapped vertically in the cavity created in the OPPy film. Further, the bacterial cavities had high selectivity and were able to discriminate particular target bacteria, Pseudomonas aeruginosa, out of bacterial mixtures containing Acinetobacter calcoaceticus, Escherichia coli, and Serratia marcescens, which are known to have a similar shape. This simple method can be used for a wide variety of applications in which rapid bacterial detection is required, such as food safety risk assessment, clinical point-of-care testing, and continuous environmental monitoring
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