期刊
ANALYTICAL CHEMISTRY
卷 84, 期 19, 页码 8277-8283出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac3017828
关键词
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资金
- National Basic Research Program of China [2010CB732403]
- National Natural Science Foundation of China [21125524, 21105012]
- Program for New Century Excellent Talents in University of China [09-0014]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
- National Science Foundation of Fujian Province [2010J06003]
Biosensors based on nanomaterials have been used for detection of various biological molecules with high sensitivity and selectivity. Herein, we developed a simple and ultrasensitive electrochemical DNA biosensor using long-range self-assembled DNA nanostructures as carriers for signal amplification, which can achieve an impressive detection limit of S aM human immunodeficiency virus (HIV) DNA even in complex biological samples. In this study, we designed two auxiliary probes. A cascade of hybridization events between the two auxiliary probes can lead to long-range self-assembly and form micrometer-long one-dimensional DNA nanostructures. In the presence of target DNA, each copy of the target can act as a trigger to connect a DNA nanostructure to a capture probe on the electrode surface. Then, a great amount of redox indicator [Ru(NH3)(6)](3+) can be electrostatically bound to the DNA nanostructures and eventually result in significantly amplified electrochemical signals.
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