Article
Chemistry, Analytical
Xuwen Gao, Xiaoxuan Ren, Yaojia Ai, Mengwei Li, Bin Zhang, Guizheng Zou
Summary: This study proposes a coreactant-free electrochemiluminescence method using mercaptopropionic acid and hexametaphosphate as the capping agents. The method achieves low-triggering-potential and coreactant-free electrochemiluminescence. The method is used for the sensitive and selective determination of molecular protein ProGRP.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Xue Lv, Lin Zhu, Yumeng Rong, Huihui Shi, Lina Zhang, Jinghua Yu, Yan Zhang
Summary: In this study, a ratiometric electrochemiluminescence lab-on-paper platform was developed for accurate and sensitive analysis of DNA methylation, based on the enhancing effect of GCAL and the quenching effect of CQDs, as well as the high conductivity copper paper electrode. The method achieved wide linear relationship and low detection limit, providing a rapid and low-cost approach for specific determination of methylated DNA.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Cheng Ma, Shaojun Wu, Yang Zhou, Hui-Fang Wei, Jianrong Zhang, Zixuan Chen, Jun-Jie Zhu, Yuehe Lin, Wenlei Zhu
Summary: The bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy allows for ECL imaging of intracellular structure and dynamic transport by using Ru(bpy)(3)(2+) and intracellular biomolecules. The research reveals the universal edge effect of cellular electroporation, as well as the dynamic transport of Ru(bpy)(3)(3+) in different cellular compartments and its correlation with the actin cytoskeleton.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Shimeles Addisu Kitte, Fuad Abduro Bushira, Tesfaye Refera Soreta
Summary: Efficient coreactants play a crucial role in electrochemiluminescence sensing. In this study, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide was demonstrated as an effective coreactant for the tris(2,2'-bipyridine)ruthenium(II) electrochemiluminescence system, allowing accurate detection of H2O2 concentration and showing satisfactory recoveries in real samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Luca Morgan, Giulio Pavan, Nicola Demitri, Chiara Alberoni, Thomas Scattolin, Marco Roverso, Sara Bogialli, Alessandro Aliprandi
Summary: A unified metal-free procedure was used to synthesize and characterize a selection of Thermally Activated Delayed Fluorescence (TADF) emitters. A promising compound with terephthalonitrile as the acceptor and diphenylamines as donors showed significantly higher electrochemiluminescence efficiency than the standard [Ru(bpy)3](PF6)2. A novel water-soluble TADF emitter (Na4[4DPASO3TPN]) was also synthesized and characterized for electrochemiluminescence in an aqueous medium.
Article
Chemistry, Analytical
Cuiying Lin, Qingqing Huang, Mengjian Tian, Fang Luo, Jian Wang, Bin Qiu, Shuofei Yang, Zhenyu Lin
Summary: An electrochemiluminescence (ECL) biosensor with dual signal enhancement was developed to detect DNA adenine methylation methyltransferase (Dam MTase). The biosensor utilized a restriction endonuclease to cleave DNA and activate CRISPR/Cas12a trans-cleavage activity, resulting in enhanced ECL signal. The biosensor exhibited a good linear relationship with Dam MTase concentration and was successfully applied for detection in serum samples.
Article
Chemistry, Analytical
Yongguang Gao, Yufei Fu, Hongyuan Li, Qiumei Feng, Yan Jiao, Baoting Dou, Po Wang
Summary: In this study, a disposable electrochemiluminescence (ECL) biosensor was designed for in situ quenching detection of methylated target DNA using target-triggered multi-DNA release and multipedal DNA walker. Zirconium(IV)-based metal organic frameworks encapsulated with Ru(bpy)32+ emitter (UiO-66NH2@Ru) were attached onto indium tin oxide (ITO) electrode as sensing substrate. Through a series of hybridization and cleavage reactions, this method achieved the quantification of methylated target DNA by quenching the ECL emission of the Ru(bpy)32+ system. This disposable biosensor holds great potential for analyzing DNA methylation levels in resource-limited environments due to its advantages of inexpensiveness, high walking kinetics, great amplification efficiency, good reproducibility, and practicability.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Physical
Kenneth Chu, Jonathan R. Adsetts, Jing Ma, Congyang Zhang, Mahdi Hesari, Liuqing Yang, Zhifeng Ding
Summary: In this study, an absolute method using a photon-counting device was developed to determine the electrochemiluminescence quantum efficiency of coreactant systems. The efficiency of the Ru(bpy)(3)(2+)/ tri-n-propylamine (TPrA) ECL coreactant system was found to be 10.0 +/- 1.1%, showing a 3-fold enhancement compared to annihilation pathways. These findings suggest a promising approach in the expanding field of ECL research.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Chemistry, Analytical
Jiaping Xie, Guomin Yang, Xingrong Tan, Ruo Yuan, Shihong Chen
Summary: This study developed a high-sensitivity detection method for the early diagnosis of Alzheimer's disease using polymeric nanoparticles as coreactant-free electrochemiluminescence emitters. By assembling these nanoparticles, along with gold nanoparticles and antibodies, on a biosensor, a sensitive analytical method for beta-amyloid(1-42) was established.
Article
Chemistry, Multidisciplinary
Ningning Wang, Hang Gao, Yunzhi Li, Guangming Li, Weiwei Chen, Zhongchao Jin, Jianping Lei, Qin Wei, Huangxian Ju
Summary: This study developed a new strategy and system using TEA-Pdots to embed coreactants in the ECL mechanism, leading to significantly improved ECL emission strength. The system can be used for in situ ECL microimaging on single living cells without the need for additional permeable treatment.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Fangshuo Liu, Kaixiang Ji, Shuai Quan, Zhenzhen Zhou, Zhiyong Dong, Altaf Hussain, Wei Zhang, Guobao Xu
Summary: Herein, a method for detecting carbonyl compounds without derivatization has been developed using bifunctional chemiluminescence coreactant thiourea dioxide, with benzaldehyde as a representative.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Li Fu, Xiancheng Liu, Ying Zeng, Qingqing Zhang, Bin Zhang, Xuwen Gao, Guizheng Zou
Summary: A promising ECL immunoassay strategy is proposed in this study, which utilizes biocompatible nanoparticles as the luminophore and achieves coreactant-free near-infrared ECL processes through electrochemical oxidation. This method exhibits high sensitivity and accuracy in the detection of tumor markers.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Rongfang Li, Guomin Yang, Ying He, Jinwen Zhao, Ruo Yuan, Shihong Chen
Summary: This study developed carboxyl-functionalized poly[9,9-bis(3'-(N,N-dimethylamino)propyl)-2,7-fluorene]-alt-2,7-(9,9 dioctylfluorene)] nanoparticles (PFN NPs) as dual-emitting luminophors, which can synchronously output strong cathodic and anodic electrochemiluminescence (ECL) signals without any exogenous coreactants. The silver nanoparticles (AgNPs) were developed as signal regulators for quenching the cathodic signal while keeping the anodic signal unchanged, and the unique dual-emitting properties of PFN NPs open up a new path for constructing coreactant-free mono-luminophor-based ECL ratio platforms.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Electrochemistry
Xiaoli Qin, Liuqing Yang, Xin Wang, Darshil Patel, Kenneth Chu, Lindsay Kelland, Jonathan Ralph Adsetts, Congyang Zhang, Mark Steven Workentin, Brian L. Pagenkopf, Zhifeng Ding
Summary: This study investigates the relative efficiencies of eight thiophene-containing compounds in the electrochemiluminescence (ECL) system and examines the impact of their structural properties on ECL efficiency. Among the studied compounds, compound 2c with benzoyl peroxide (BPO) as a coreactant exhibits the highest relative ECL efficiency. Additionally, the ECL efficiency in the potential pulsing/ECL experiment is 6 times larger than that in the potentiodynamic experiment.
Article
Chemistry, Applied
Haiyang Wang, Lin Cai, Wen Hao, Yuwei Wang, Guozhen Fang, Shuo Wang
Summary: A novel self-enhancement molecularly imprinted electrochemiluminescence (ECL) sensor (MIP/M-Ag@MoS2QDs/GCE) was constructed for the detection of thiabendazole (TBZ) in food. The composite nanomaterials (M-Ag) were prepared by chelating Ag+ with melamine as the template. M-Ag exhibited both ECL properties and coreactant catalytic properties, enabling the self-enhancement of ECL luminophore. MoS2-QDs with excellent edge activity and electrochemical reaction catalytic activity were utilized to accelerate the reaction rate and further enhance the ECL intensity.
Article
Instruments & Instrumentation
Shunsuke Tomita, Ryoji Kurita
Summary: The study introduces a pattern-recognition-based protein sensing approach using a simple one-component array composed of charged poly-L-lysine modified with environment-responsive dansyl fluorophores for the identification of proteases. By dissolving the array of PLL-Dnc in buffer solutions with different pH values, fluorescent patterns unique to each protease are produced, enabling the identification of eight proteases through multivariate analysis. The method allows discrimination of mixtures of trypsin and alpha(1)-antitrypsin (ATT) with different compositions, and is expected to have broad applications in screening protein-protein complexes formation and disease-associated protease detection.
SENSORS AND MATERIALS
(2021)
Article
Chemistry, Analytical
Hiroki Okada, Masahiro Mimura, Shunsuke Tomita, Ryoji Kurita
Summary: The study introduces a novel strategy of using chemical additives to modulate probe/analyte interactions for constructing pattern-recognition-based sensing systems. The enhanced fluorescence of dansyl-modified cationic poly-L-lysine upon binding to proteins generates response patterns that accurately identify structurally similar albumin homologues and mammalian cells through multivariate analysis. This inexpensive and accessible strategy offers new opportunities for bioanalytes detection.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Shota Takahashi, Hiroyuki Yanagisawa, Tomoyuki Kamata, Dai Kato, Ryoji Kurita, Shunsuke Shiba, Osamu Niwa
Summary: A research team developed carbon film electrodes using UBM and ECR sputtering equipment for electroanalysis of heavy metal ions. Different methods were used for detection of different heavy metal ions, achieving good detection limits suitable for environmental analysis.
Article
Chemistry, Analytical
Dai Kato, Tomoyuki Kamata, Michinori Sumimoto
Summary: By studying nanocarbon film electrodes, we successfully detected tryptophan metabolites, particularly kynurenic acid (KYNA), due to the superior electrode properties and unique structure of the nanocarbon film. The sp(2)/sp(3) ratio of the nanocarbon film plays a crucial role in electrode activity, potential window, and electrode performance, with nanocarbon films containing high sp(3) content exhibiting better performance and theoretical oxidation potential values matching experimental results.
Article
Plant Sciences
Tatsunosuke Tomita, Renu Wadhwa, Sunil C. Kaul, Ryoji Kurita, Naoshi Kojima, Yoshiaki Onishi
Summary: Withanolide derivatives, particularly 3 beta mWi-A, have been shown to lengthen the circadian clock, upregulate the expression of key genes involved in circadian rhythms, and act as an inverse agonist for RORa. These findings suggest that 3 beta mWi-A may serve as a novel modulator of circadian rhythms.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Masahiro Mimura, Shunsuke Tomita, Yoichi Shinkai, Takuya Hosokai, Hiroyuki Kumeta, Tomohide Saio, Kentaro Shiraki, Ryoji Kurita
Summary: Recent studies have shown that DNA quadruplex folding plays a key role in the formation of liquid droplets through interactions with linker histone H1, inducing liquid-liquid phase separation. Quadruplex DNA structures promote droplet formation and decrease molecular motility within the droplets.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Cell Biology
Masahiro Mimura, Shunsuke Tomita, Hiroka Sugai, Yoichi Shinkai, Sayaka Ishihara, Ryoji Kurita
Summary: This study demonstrates the roles of DNA components in liquid-liquid phase separation of proteins and DNAs, including electrostatic interactions between phosphate groups and deoxyribose, as well as interactions involving nucleobases and deoxyriboses. These interactions promote the generation of spherical liquid droplets and modulate the density of DNA within the droplets under low-salt conditions. Additionally, non-spherical assemblies with both mobile and immobile fractions were observed at higher concentrations of a polycationic peptide, linker histone H1, for all DNA oligomers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Dai Kato, Johtaro Yamamoto, Yoshio Suzuki, Tomoyuki Kamata, Hinako Hashimoto, Masashi Kunitake
Summary: The study investigated the diffusion properties of lipophilic vitamin E through bicontinuous microemulsions (BME) using electrochemical and fluorescence correlation spectroscopy (FCS) measurements. Results showed that a higher VE diffusion coefficient and faster diffusion were observed in the oil-rich BME, indicating superior electrochemical measurements can be achieved by controlling the conditions of the BME as the measurement electrolyte.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shunsuke Shiba, Ayaka Koike, Shota Takahashi, Dai Kato, Tomoyuki Kamata, Osamu Niwa
Summary: A method for the synthesis of highly oriented metallic heterodimer arrays on a metal nanoparticle-embedded carbon film using a selective electrodeposition technique is proposed. This method does not require the use of surfactants, capping agents, organic solvents, or heat treatment. Selective electrodeposition is achieved by utilizing the difference in electrodeposition overpotentials between carbon and Pd nanoparticle surfaces.
Article
Electrochemistry
Shunsuke Shiba, Soichiro Yoshimoto, Shunta Hashiguchi, Masashi Kunitake, Dai Kato, Osamu Niwa, Masanobu Matsuguchi
Summary: This study reports a method of preparing porous gold nanomesh film through gold electrodeposition in a bicontinuous microemulsion. Toluene selectively adsorbs on the Au surface, inducing anisotropic 2D growth of gold, and the nanochannels in the microemulsion form abundant in-plane nanopores.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Physical
Kodai Nakao, Koji Noda, Hinako Hashimoto, Mayuki Nakagawa, Taisei Nishimi, Akihiro Ohira, Yukari Sato, Dai Kato, Tomoyuki Kamata, Osamu Niwa, Masashi Kunitake
Summary: Bicontinuous microemulsions (BMEs) have been investigated as unique heterogeneous mixtures for electrochemistry. A three-dimensionally expanded ITIES-BME system was successfully constructed, and electrochemical reactions were observed to occur in both immiscible solution phases. This research opens up possibilities for applications such as electrolysis synthesis and secondary batteries.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Shunsuke Tomita, Hiroyuki Kusada, Naoshi Kojima, Sayaka Ishihara, Koyomi Miyazaki, Hideyuki Tamaki, Ryoji Kurita
Summary: This study developed a sensing system based on the chemical-nose strategy to transduce the characteristics of microbiota into fluorescence patterns. By using pattern-recognition algorithms to analyze the fluorescence patterns, different intestinal bacterial strains can be identified, and obesity-associated and sleep disorder-associated gut microbiota can be detected.
Article
Chemistry, Multidisciplinary
Shunsuke Shiba, Saki Ohta, Kazuya Ohtani, Shota Takahashi, Dai Kato, Osamu Niwa
Summary: The supporting effect of N-doped carbon film improved the crystallinity of electrodeposited Ni@Ni(OH)(2) core-shell nanoparticles, leading to a higher regeneration rate of catalytic sites and oxidation currents of sugars, as well as a significant decrease in overpotential for the G5 oxidation reaction, possibly due to electrostatic interaction between NPs and G5.
Article
Nanoscience & Nanotechnology
Shunsuke Shiba, Shoei Hirabayashi, Osamu Niwa, Dai Kato, Masashi Kunitake, Masanobu Matsuguchi
ACS APPLIED NANO MATERIALS
(2020)
Article
Chemistry, Analytical
Ryo Nishihara, Ryoji Kurita
Summary: The study developed a luciferase-independent copper detection platform using a novel luminogenic caged coelenterazine analogue (TPA-H1) to detect free copper ions in serum with high specificity and sensitivity. This new method can be a simple mix-and-read approach for quantitatively analyzing copper ions.