Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Rintaro Saito, Masahiro Sugimoto, Akiyoshi Hirayama, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: Large-scale metabolomic studies are common, but there is less focus on the reliability of uncharacterized peaks in untargeted metabolomics data. This study tested criteria to assess the reliability of uncharacterized peaks and identified some reliable peaks potentially related to important physiological processes like disease progression.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Food Science & Technology
Maria Castro-Puyana, Maria L. Marina
Summary: This review article critically examines the significant advances in using CE-MS-based approaches to assess various aspects of Food Science, and discusses the latest developments in hardware and software.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno, Shigeyuki Kawachi, Makoto Sunamura, Masahiro Sugimoto
Summary: Kidney biopsy is commonly used to diagnose kidney transplant dysfunction. In this study, capillary electrophoresis-mass spectrometry was used to analyze the biofluids of transplant recipients. Metabolomics analyses revealed significant differences in the metabolomic profiles of the different groups, particularly in arginine biosynthesis and purine metabolism in plasma samples. Different types of kidney disorders also showed distinct metabolomic profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Marlien van Mever, Magdalena Fabjanowicz, Maricruz Mamani-Huanca, Angeles Lopez-Gonzalvez, Justyna Plotka-Wasylka, Rawi Ramautar
Summary: The composition of wine is influenced by environmental factors, genetic factors, and winemaking practices. Metabolomics using NMR spectroscopy, GC-MS, and/or LC-MS is a useful approach for assessing the origin, authenticity, and quality of wines. This study demonstrates that CE-MS is a suitable method for analyzing polar ionogenic metabolites in wine.
Article
Biochemical Research Methods
Paniz Jasbi, Xiaojian Shi, Ping Chu, Natalie Elliott, Haley Hudson, Douglas Jones, Geidy Serrano, Brandon Chow, Thomas G. Beach, Li Liu, Garilyn Jentarra, Haiwei Gu
Summary: This study conducted metabolomics analysis on brain tissues of dementia patients, identifying metabolite markers that can distinguish different cases, providing important clues for understanding disease mechanisms and identifying therapeutic targets, laying the foundation for future research on the diagnosis and treatment of Alzheimer's disease.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Chemistry, Analytical
Tomoyoshi Soga
Summary: Capillary electrophoresis mass spectrometry (CE-MS) is a powerful tool for comprehensive analysis of low-molecular-weight charged metabolites. This review discusses the strategies and principles of CE-MS-based metabolomics analyses, including sample preparation, CE separation modes, interfacing techniques, and mass spectrometers. Additionally, it highlights new techniques to increase throughput ability in CE-MS for metabolomics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Jericha Mill, Vihar Patel, Ozioma Okonkwo, Lingjun Li, Thomas Raife
Summary: Diagnosing Alzheimer's disease in the early stage is challenging. This study explores the potential utility of erythrocyte metabolite profiles as biomarkers for screening AD. Comparing the metabolomes of AD patients and control group, increased amino acid metabolism and decreased lipid metabolism were observed in AD. Sphingolipids, previously implicated in AD, were consistently decreased in AD patients, suggesting their potential as risk markers for AD development.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Zi-Ao Huang, Jiahua Tan, Yueyang Li, Siyu Miao, Kymora B. Scotland, Ben H. Chew, Dirk Lange, David D. Y. Chen
Summary: Migration time correction is crucial in CE-based metabolomics, especially for dual pressure CE. We proposed a new correction method and developed a script tool for migration time correction and peak alignment. A case study demonstrated the effectiveness of this method.
Article
Biochemistry & Molecular Biology
Samyukta Sah, Sylvia R. Yun, David A. Gaul, Andro Botros, Eun Young Park, Olga Kim, Jaeyeon Kim, Facundo M. Fernandez
Summary: In this study, a targeted microchip CE-HRMS method was developed to analyze multiple metabolites in mouse serum samples, revealing metabolic alterations during the development and progression of high-grade serous carcinoma.
Article
Chemistry, Analytical
Qinlei Liu, Sandra Martinez-Jarquin, Wenjie Ge, Renato Zenobi
Summary: Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susanna Della Posta, Chiara Fanali, Valeria Gallo, Salvatore Fanali
Summary: This paper presents recent results obtained by coupling electromigration techniques with mass spectrometry, highlighting the advantages of this hybrid approach and discussing selected applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Bingshu He, Wei Zhang, Faisa Guled, Amy Harms, Rawi Ramautar, Thomas Hankemeier
Summary: This paper discusses the crucial factors for the performance of biomass-restricted metabolomics studies, including sampling and sample preparation methods, separation techniques, and ionization sources. It also provides overviews of MS-based miniaturized metabolomics studies reported over the past five years, with information on sample type, sample preparation volume, injection volume, separation techniques, and MS analyzers.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Meera Shanmuganathan, Mohammad Omair Sarfaraz, Zachary Kroezen, Holly Philbrick, Richel Poon, Andrew Don-Wauchope, Marco Puglia, David Wishart, Philip Britz-McKibbin
Summary: Metabolomics using MSI-CE-MS and NMR techniques compared serum metabolome of HCV patients with liver fibrosis and non-HCV controls, revealing elevated serum choline and histidine in late-stage liver fibrosis. Serum choline to uric acid ratio provided optimal differentiation of liver disease severity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Applied
Jose David Sanchez-Martinez, Gerardo Alvarez-Rivera, Rocio Gallego, Mariane Bittencourt Fagundes, Alberto Valdes, Jose A. Mendiola, Elena Ibanez, Alejandro Cifuentes
Summary: The recovery of terpenoids from orange by-products was improved by optimizing the conditions of pressurized liquid extraction (PLE). The PLE extracts showed high neuroprotective potential and exhibited antioxidant, anti-cholinesterase, and anti-inflammatory properties. They also protected against L-glutamic acid in cell models.
Article
Food Science & Technology
Maria Isabel Landim Neves, Alberto Valdes, Eric Keven Silva, Maria Angela A. Meireles, Elena Ibanez, Alejandro Cifuentes
Summary: This study investigates the synthesis of natural blue colorants. The crosslinking reaction between genipin and amino acids can form a blue pigment. By studying the reaction kinetics and conditions between different amino acids and genipin, the researchers aim to optimize the synthesis process.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Norelhouda Abderrezag, Zully J. Suarez Montenegro, Ouahida Louaer, Abdeslam-Hassen Meniai, Alejandro Cifuentes, Elena Ibanez, Jose A. Mendiola
Summary: This study successfully applied Gas Expanded Liquid (GXL) extraction to concentrate beneficial compounds from Silybum marianum seeds, determining optimal extraction conditions and revealing significant antioxidant and anti-inflammatory activities in the obtained extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Chongting Guo, Alberto Valdes, Jose David Sanchez-Martinez, Elena Ibanez, Jinfeng Bi, Alejandro Cifuentes
Summary: In this study, a pressurized liquid extraction method was optimized and applied to thinned peaches dried under different conditions to evaluate their neuroprotective potential. The extracts obtained from freeze-dried samples with 50% ethanol in water at 180 degrees C showed the best neuroprotective potential. The identified metabolites, including isoferulic acid, 4-methyldaphnetin, coniferyl aldehyde, and 3,4-dihydroxyacetophenone, were able to cross the blood-brain barrier and were positively correlated with the neuroprotective potential.
Review
Nutrition & Dietetics
Romulo Alves Morais, Gerson Lopes Teixeira, Sandra Regina Salvador Ferreira, Alejandro Cifuentes, Jane Mara Block
Summary: Fruits from the Arecaceae family are rich in nutrients and bioactive compounds with potential health benefits. However, research on these fruits is still limited. Understanding their composition and health effects is important for promoting production and consumption, as well as improving the economy and social condition of the regions where they are found.
Article
Biochemistry & Molecular Biology
Jose David Sanchez-Martinez, Ana Rita Garcia, Gerardo Alvarez-Rivera, Alberto Valdes, Maria Alexandra Brito, Alejandro Cifuentes
Summary: Agrifood by-products and microalgae are rich sources of bioactive compounds with neuroprotective properties, but their effectiveness is limited by the blood-brain barrier (BBB). In this study, green extracts from various sources were tested for their permeability across an in vitro BBB model. Results showed that neuroprotective compounds like terpenoids, phytosterols, and phenolic compounds had high permeability. These findings support the potential use of these natural extracts as functional ingredients in food supplements or nutraceuticals with neuroprotective claims.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Juliana A. Barreto Peixoto, Gerardo alvarez-Rivera, Anabela S. G. Costa, Susana Machado, Alejandro Cifuentes, Elena Ibanez, M. Beatriz P. P. Oliveira, Rita C. Alves
Summary: This study investigated the antioxidant potential, phenolic profile, and free amino acid profile of lemon verbena infusions from different commercial brands. Various assays showed that the infusions exhibited high antioxidant activity, which was correlated with total phenolics and total flavonoid contents. Phenylalanine also appeared to have an impact on the antioxidant activity. Significant differences were found among the evaluated parameters for one of the commercial brands, possibly due to different geographical origins. Overall, lemon verbena infusions contain a variety of bioactive compounds with therapeutic potential.
Article
Food Science & Technology
Joao V. B. Borsatto, Edvaldo V. S. Maciel, Alejandro Cifuentes, Fernando M. Lancas
Summary: Sample preparation is a complex and time-consuming procedure that directly affects analysis quality. Recent studies have explored online extraction coupled with liquid chromatography-mass spectrometry (OLE-LC-MS) as a promising alternative for analyzing analytes in complex solid matrices. In this study, the lecithin profile in various seeds was analyzed using OLE-LC-MS, demonstrating its feasibility without the need for offline solvent extraction. Optimization of the extraction phase gradient method and exploration of different analytical columns improved separation and repeatability. Overall, the OLE-LC-MS approach showed potential for lipidomics analysis in the future.
Article
Neurosciences
Vilma Alanko, Adhara Gaminde-Blasco, Tania Quintela-Lopez, Raul Loera-Valencia, Alina Solomon, Ingemar Bjorkhem, Angel Cedazo-Minguez, Silvia Maioli, Graziella Tabacaru, Maria Latorre-Leal, Carlos Matute, Miia Kivipelto, Elena Alberdi, Anna Sandebring-Matton
Summary: Oxidized cholesterol metabolite 27-hydroxycholesterol (27-OH) is a potential link between hypercholesterolemia and neurodegenerative diseases as it can cross the blood-brain barrier. This study found that high levels of 27-OH can impact oligodendrocyte function and contribute to the disconnection of neural networks in neurodegenerative diseases.
Review
Chemistry, Analytical
Brenda Lee Simas Porto, Alberto Valdes, Alejandro Cifuentes, Gerardo Alvarez-Rivera
Summary: This article revisits recent articles that have used capillary electromigration techniques to study various phytochemicals between 2018 and 2021. The manuscript is divided based on the different separation methods used, and the main aspects of each application are briefly discussed, including sample preparation, advantages and disadvantages of the developed methods, problems encountered during the process, and ways to solve them.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Gloria Dominguez-Rodriguez, Lidia Montero, Miguel Herrero, Alejandro Cifuentes, Maria Castro-Puyana
Summary: This study provides an overview of the main applications of capillary electromigration (CE) methods in food analysis and Foodomics. Papers published between March 2021 and March 2023 were reviewed. The study highlights the various CE methods that have been developed and utilized for the analysis of different molecules in food and beverages. Additionally, new CE procedures for chiral separation, evaluating the effects of food processing, and recent developments in microchip CE and Foodomics applications are discussed.
Article
Biochemistry & Molecular Biology
Joao Victor Basolli Borsatto, Edvaldo Vasconcelos Soares Maciel, Alejandro Cifuentes, Fernando Mauro Lancas
Summary: Graphene-based sorbents were used as extraction and analytical columns in a column-switching system. The system showed successful application for pyriproxyfen analysis but was not suitable for abamectin B1a analysis. The SiGO-C18ec column presented significant tailing compared to a C18 column in a similar approach.
Article
Food Science & Technology
Pouya Mohammadnezhad, Alberto Valdes, Ruth E. Barrientos, Elena Ibanez, Jane Mara Block, Alejandro Cifuentes
Summary: In this study, the lipidic fraction of Amazonian Pentaclethra macroloba nuts was chemically characterized using supercritical fluid extraction. The results suggest that the by-products of pracaxi oil extraction are a valuable source of bioactive compounds with neuroprotective potential.
Article
Pharmacology & Pharmacy
Maria Taboada-Alquerque, Danilo Pajaro-Valenzuela, Karina Caballero-Gallardo, Alejandro Cifuentes, Elena Ibanez, Maicol Ahumedo-Monterrosa, Elena E. Stashenko, Jesus Olivero-Verbel
Summary: A natural compound called carnosol found in rosemary has been identified as a potential anti-tumor and anti-inflammatory agent. Through network pharmacology, plausible human targets for carnosol were identified, and it was found to be closely related to infection and inflammation. These findings suggest that carnosol may exert its effects through inducing an immuno-inflammatory response.
SCIENTIA PHARMACEUTICA
(2023)
Review
Chemistry, Analytical
Gerson-Dirceu Lopez, Gerardo Alvarez-Rivera, Chiara Carazzone, Elena Ibanez, Chad Leidy, Alejandro Cifuentes
Summary: Natural carotenoids have important applications in pharmaceutical, cosmetic, nutraceutical, and food industries. The use of bacteria for carotenoid production has gained much attention in recent years. This review summarizes the methods for extracting and identifying bacterial carotenoids and highlights the use of high-performance liquid chromatography-mass spectrometry (HPLC-MS) for analysis and identification. The potential commercial use of these carotenoids is also discussed.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)