Article
Multidisciplinary Sciences
Amir Shamloo, Amin Naghdloo, Mohsen Besanjideh
Summary: The study focused on isolating rare cancer cells using two centrifugal microfluidic devices. The passive design isolates cells through inertial effects and bifurcation law, while the hybrid design captures magnetically labeled cells using permanent magnets at the end of the microchannel. The hybrid design offers better separation efficiency and purity, while the passive one is simpler and cost-effective without the need for cell labeling.
SCIENTIFIC REPORTS
(2021)
Article
Optics
Xinyu Chen, Lauren Waller, Jiajie Chen, Rui Tang, Zunming Zhang, Ivan Gagne, Bien Gutierrez, Sung Hwan Cho, Chi-Yang Tseng, Ian Y. Lian, Yu-Hwa Lo
Summary: The newly developed Bessel-Gaussian beam image-guided cell sorter has an ultra-long depth of focus, enabling focused images of over 85% of passing cells. It utilizes characteristics extracted from the output temporal waveform of a photomultiplier tube (PMT) detector for label-free sorting based on cell images, achieving sorting purities of 97% for polystyrene beads, 97% for SKNO1 leukemia cells, and 98% for Scenedesmus green algae.
Article
Chemistry, Multidisciplinary
Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E. Pimanda, Peggy P. Y. Chan, Vashe Chandrakanthan
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Article
Biochemical Research Methods
Yameng Liu, Miaomiao Wang, Ruojin Liu, Feng Qiu
Summary: This study aims to develop a simple and fast method for testing the phagocytic activity of unlabeled and native cells using high-resolution direct current insulator-based dielectrophoresis (DC-iDEP). The method reduces the time and cost of bacterial culture and fluorescent labeling, and the introduction of microfluidics reduces sample volume and reagent needed. The analysis of the biophysical property distributions of cells may lead to a cheaper and faster tool for evaluating medicinal effects. Furthermore, the study investigates the effect of berberine on reducing macrophage phagocytic activity for comparison.
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Lin Zeng, Xi Chen, Rongrong Zhang, Shi Hu, Hongpeng Zhang, Yi Zhang, Hui Yang
Summary: In this study, a microfluidic system based on negative magnetophoresis is proposed for the high-resolution separation of nanoparticles. The system can purify 200 nm particles with high purity and recovery rate. Compared with existing methods, the system shows significant improvements in particle size resolution and sample processing throughput.
Article
Chemistry, Analytical
Shao-Li Hong, Nangang Zhang, Li Qin, Man Tang, Zhao Ai, Aiju Chen, Shuibing Wang, Kan Liu
Summary: An automated detection device for H7N9 influenza virus hemagglutinin was constructed using 3-D magnetophoretic separation and magnetic label, achieving a detection limit of 8.4 ng mL(-1) with good specificity and reproducibility. The device proposes promising automated avenues for early infectious disease detection.
Article
Materials Science, Multidisciplinary
Tamaghna Gupta, Rohit Gupta, Mohammadhossein Dabaghi, Rakesh P. Sahu, Jeremy A. Hirota, Ishwar K. Puri
Summary: This study presents a rapid, insert-free magnetic exclusion technique using magnetic fields to create reproducible cell aggregates. The technique is inexpensive, easy to use, and amenable to automation, with potential applications in cancer research, high throughput drug discovery, and screening.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biology
Jose Montoya Mira, Ajay A. Sapre, Brett S. Walker, Jesus Bueno Alvarez, Kyle T. Gustafson, Eugene Tu, Jared M. Fischer, Melissa H. Wong, Sadik Esener, Yu-Jui Chiu
Summary: Researchers have developed a label-free dielectrophoretic microfluidic platform for the enrichment of circulating hybrid cells (CHCs), showing promising potential for isolating tumor cells non-invasively from patient blood samples within one hour. They have optimized the device using in vitro models and successfully applied it to clinically relevant mutation analyses in 75% of the samples tested.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Lap Man Lee, George J. J. Klarmann, Dustin W. W. Haithcock, Yi Wang, Ketan H. H. Bhatt, Balabhaskar Prabhakarpandian, Kapil Pant, Luis M. M. Alvarez, Eva Lai
Summary: Human adipose tissue is a valuable source of mesenchymal stem cells (MSCs) and a microfluidic sorter cascade has been developed to enrich and purify adipose-derived stem cells (ADSCs) from tissue-digested samples. The sorter cascade utilizes spiral-shaped inertial and deterministic lateral displacement (DLD) sorters to separate cells based on size difference, resulting in a >90% separation efficiency. The enriched ADSC sub-population shows a 6x enhancement of expansion capacity in tissue culture. This microfluidic sorter cascade holds promise for various applications in tissue engineering and regeneration medicine.
Article
Chemistry, Multidisciplinary
Tun Cao, Zhongming Wang, Libang Mao
Summary: This study presents an optical sorting method for polystyrene particles using optofluidic nanophotonic paired waveguide (ONPW) composed of chalcogenide semiconductor Sb2Se3. The coupling length between hot spots in the ONPW can be modulated by changing the state of Sb2Se3, allowing for the trapping and sorting of particles with different shapes. This scheme may provide a new approach for shape-selective sieving of biomolecules and meet the requirements of new-generation lab-on-chip techniques.
Review
Chemistry, Analytical
Roozbeh Abedini-Nassab, Negar Sadeghidelouei, C. Wyatt Shields
Summary: Lab-on-a-chip tools are crucial in advancing modern biology and medicine, particularly in the precise transport of single particles and cells for analysis. Magnetophoretic circuits have emerged as a unique technology for manipulating individual bioparticles in a parallel and controlled manner. This review discusses theoretical considerations, provides a tutorial on operating magnetophoretic devices, compares their utility with other microchip-based platforms, and explores their potential in addressing unmet needs in single-cell biology and medicine.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Anton Mukhamedshin, Riley C. Reddington, Mai T. P. Dinh, Kumar Abhishek, Mubasher Iqbal, Marc Manheim, Sean C. Gifford, Sergey S. Shevkoplyas
Summary: The study introduces a closed-loop microfluidic device that effectively enriches T cells while removing monocytes and platelets, offering a simplified and standardized manufacturing process for cellular therapies.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemical Research Methods
Jia Yang, Yanyu Gu, Chuang Zhang, Yuzhao Zhang, Wenfeng Liang, Lina Hao, Ying Zhao, Lianqing Liu, Wenxue Wang
Summary: This study presents an optically-induced dielectrophoresis (ODEP)-based approach for efficient and nondestructive label-free purification and characterization of optogenetically engineered cells, specifically HEK293-ChR2 cells. The distinctive crossover frequencies of the engineered cells and unmodified cells enable effective separation using opposite DEP forces. The ODEP-based approach significantly improves the purity of the separated cell population and enhances the imaging quality.
Article
Engineering, Environmental
Gregory Parisi, Shankar Narayan
Summary: The study presents a novel approach of using a fluorine-free superhydrophobic/superoleophilic copper mesh for efficient oil-water separation, achieving high separation fluxes and efficiencies. The material is cost-effective, durable, and demonstrates a simple strategy to switch between different wetting states for selective separation of oil and water. This technology has the potential to address oily wastewater challenges in industrial applications and oil-spill situations with further development.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Chemistry, Analytical
Ga-Yeong Kim, Jaejung Son, Jong-In Han, Je-Kyun Park
Summary: This study successfully utilized a contraction-expansion array microchannel for size-based separation of microalgae without the need for highly skilled operators and months of repeated separation, demonstrating a more efficient alternative to conventional microalgae separation technologies.
Article
Biochemical Research Methods
Jaejung Son, Hee-Hoon Kim, Jun-Hee Lee, Won-Il Jeong, Je-Kyun Park
Summary: A novel 3D tissue-level hepatic cell culture platform has been developed in this study, which is expected to be used for in-depth analysis of drug toxicity in complex tissues.
Article
Biophysics
Juhwan Park, Kyoung G. Lee, Dong Hyun Han, Ji-Soo Lee, Seok Jae Lee, Je-Kyun Park
Summary: The study introduces a portable microfluidic device, PAMD, for generating and dispensing droplets for ddPCR with a simple pushbutton operation. PAMD can control multiple droplet generation units simultaneously with a single pushbutton, and the size of droplets can be adjusted by changing the geometry of the droplet generation channel.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yoo Min Park, Juhwan Park, Sun Young Lim, Yeji Kwon, Nam Ho Bae, Je-Kyun Park, Seok Jae Lee
Summary: The study developed a simple single-chip pathogen analysis system by integrating different modules for gene amplification, electrochemical analysis, and sample migration and mixing without external pumping systems. The system successfully detected target pathogens in laboratory conditions, demonstrating practicality and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Juhwan Park, Je-Kyun Park
Summary: This Perspective discusses the advantages and limitations of microfluidic technologies in sample preparation, focusing on the potential and future development of pushbutton-activated microfluidic technologies as user-friendly sample preparation tools. It briefly reviews the working principle and recent advances in pushbutton-activated microfluidic technologies, and discusses future prospects for wide application in terms of integration with the signal analysis system, user-dependent variation, and universal and facile use.
Article
Engineering, Biomedical
Gihyun Lee, Soo Jee Kim, Honggu Chun, Je-Kyun Park
Summary: Microextrusion bioprinting has developed a multiple-bioink printing system by integrating a multibarrel nozzle for extruding multiple bioinks with a nebulizer for simultaneous crosslinking. This innovative system allows continuous extrusion of various biomaterials while stabilizing the printed constructs through aerosol-based crosslinking, improving printing resolution and stability in creating complex tissues with compartmentalized regions.
Article
Materials Science, Multidisciplinary
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: This study proposes a novel bioprinting technique using a supportive microstructured bioprinting substrate to enhance the structural fidelity of low-viscosity hydrogel constructs and replicate the biological vessel formation process. This technology could be applied to developing complex constructs using cytocompatible natural hydrogels.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biophysics
Jieun Han, Hye Jin Lim, Juhwan Park, Dong Hyun Han, Dong-Myung Kim, Je-Kyun Park
Summary: A dual sensing microfluidic device has been developed for fast, portable, and quantitative analysis of target amino acids, using the biological mechanism of protein synthesis. This device enables precise quantification of amino acids within 30 min at room temperature, indicating its potential for point-of-care testing of metabolic compounds.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Dong Hyun Han, Untaek Oh, Je-Kyun Park
Summary: Three-dimensional (3D) printing is a promising technology for fabricating microstructures, but its direct use as microchannels is limited. In this study, we replicated PDMS microchannels using 3D-printed molds and found that bonding two single PDMS layers significantly increased the optical transmittance. We evaluated the applicability of the replicated PDMS devices by comparing them to conventional Si-wafer molds and performed droplet generation in the PDMS microchannels. This study provides a fundamental understanding of prototyping microstructures from DLP-based 3D-printed molds.
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks is proposed. The approach involves the use of a micromesh substrate onto which PLA filament is extruded followed by the printing of fibrinogen. The micromesh supports the printed hydrogel with a capillary pinning effect, enabling high-resolution bioprinting. The 3D MOSAIC platform allows the fabrication of complicated and multimaterial 3D structures and the self-organization of cells within the hydrogel.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Summary: The tumor microenvironment (TME) is crucial for the nourishment of tumors and delivery of chemotherapy drugs, but existing bioprinting and microfluidic technologies have not fully utilized the potential benefits of precise spatial control and adjustment of culture environments. This study introduces a novel integrative technology that combines a self-organized TME array bioprinted on a microfluidic chip with a vascular endothelial barrier surrounding breast cancer spheroids.
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks was proposed. The microextrusion printing of fibrinogen onto PLA-framed micromesh substrates allowed for high-resolution bioprinting. The resulting 3D constructs could support the self-organization of endothelial cells and enable cancer cell migration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. A continuous multiple-material bioprinting system is described that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment.
Article
Biochemical Research Methods
Hwisoo Kim, Hyewon Roh, Haseong Kim, Je-Kyun Park
Summary: Hanging drop plates and low-attachment well plates are suitable for high throughput screening of spheroids, but uniform culture is difficult. To address this issue, researchers have developed a PDMS-based chip and pillar array chip, using a droplet contact-based technique for multiple reagent changes or washings.
Article
Biochemical Research Methods
Chang Hyun Cho, Minkyung Cho, Je-Kyun Park
Summary: The study introduced a multichannel microfluidic immunohistochemistry technology for high-throughput analysis of tissue microarrays, showing great potential for molecular diagnostics.
