Article
Materials Science, Biomaterials
Jyoti, Renata Rybakiewicz-Sekita, Teresa Zolek, Dorota Maciejewska, Edyta Gilant, Katarzyna Bus-Kwasnik, Andrzej Kutner, Krzysztof R. Noworyta, Wlodzimierz Kutner
Summary: An electrochemical chemosensor for cilostazol determination was developed, showing high selectivity and sensitivity within a linear concentration range of 50 to 924 nM. The use of quantum-mechanical and molecular dynamics analysis in pre-polymerization complexes helped select the most appropriate monomer, and simulated models predicted the sensor's selectivity. The chemosensor was also effective in determining cilostazol and its metabolite in spiked human plasma, demonstrating good recovery rates and a low limit of detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Nanoscience & Nanotechnology
Nabila Yasmeen, Aneta Karpinska, Jakub Kalecki, Wlodzimierz Kutner, Karina Kwapiszewska, Piyush S. Sharma
Summary: Biocompatible polyacrylamide gel and core-shell nanoparticles were synthesized using a one-step electro-chemically initiated gelation. The core-shell nanoparticles exhibited superior support for complex 3D tissue structures.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Katarzyna Bartold, Zofia Iskierko, Pawel Borowicz, Krzysztof Noworyta, Chu-Yun Lin, Jakub Kalecki, Piyush Sindhu Sharma, Hung-Yin Lin, Wlodzimierz Kutner
Summary: A conducting molecularly imprinted polymer (MIP) film integrated with an extended-gate field-effect transistor (EG-FET) transducer was used to selectively determine epitopes of matrix metalloproteinase-1 (MMP-1) protein biomarker for idiopathic pulmonary fibrosis (IPF). The most suitable epitopes were selected using Basic Local Alignment Search Tool software, and MIPs templated with these epitopes were prepared and deposited as thin films on electrodes. The chemosensors constructed from these MIP films and EG-FET proved useful in determining the epitopes even in complex media. The chemosensors selectively recognized whole MMP-1 protein in the concentration range of 50-500 nM in buffered control serum samples, with a limit of detection of ~60 nM and 20 nM for the two selected epitopes, respectively.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Food Science & Technology
Viknasvarri Ayerdurai, Patrycja Lach, Agnieszka Lis-Cieplak, Maciej Cieplak, Wlodzimierz Kutner, Piyush Sindhu Sharma
Summary: In the era of global market, controlling food product quality is challenging. This review emphasizes the importance of developing fast, robust, and cost-effective analytical assays to determine food contamination. The application of molecularly imprinted polymers (MIPs) in selective assays for various food products is discussed.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Electrochemistry
Jyoti, Evgenia Dmitrieva, Teresa Zlolek, Dorota Maciejewska, Renata Rybakiewicz-Sekita, Wlodzimierz Kutner, Krzysztof R. Noworyta
Summary: This study investigates the electrochemical polymerization behavior of two carbazole derivatives, CNZ1 and CNZ2. The formation of thin poly(CNZ1) film and thick poly(CNZ2) film, as well as the difference in UV-vis-NIR absorption intensity and radical formation, suggests different behavior between the two monomers. The study combines spectroelectrochemistry, scanning electron microscopy, and quantum-chemistry calculations to shed light on the observed differences.
ELECTROCHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Viknasvarri Ayerdurai, Maciej Cieplak, Wlodzimierz Kutner
Summary: Food safety assessment plays a crucial role in the modern food industry, driven by increased public awareness and the rise in food pollutants due to globalization, industrialization, and population growth. Electrochemical sensors with molecularly imprinted polymer (MIP) recognition units have become a popular choice for food analysis. These sensors offer simplicity in preparation and measurement, cost-efficiency, and a high affinity for target contaminants. The review highlights recent developments in MIP-based electrochemical sensors and their applications in detecting contaminants in various food and drink samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mei-Hwa Lee, Cheng-Chih Lin, Piyush Sindhu Sharma, James L. Thomas, Chu-Yun Lin, Zofia Iskierko, Pawel Borowicz, Chien-Yu Lin, Wlodzimierz Kutner, Chien-Hsin Yang, Hung-Yin Lin
Summary: Instead of imprinting the entire protein molecule, imprinting protein epitopes is becoming more popular. MMP-1, belonging to the matrix metalloproteinase protein family, can serve as a disease protein biomarker. Epitopes of MMP-1 were identified and used to create a molecularly imprinted polymer. The polymer was optimized for conductivity and used to accurately determine MMP-1 concentration, which can be useful for early diagnosis.
Article
Materials Science, Multidisciplinary
Jaroslaw Mazuryk, Katarzyna Klepacka, Joanna Piechowska, Jakub Kalecki, Ladislav Derzsi, Piotr Piotrowski, Piotr Paszke, Dorota A. Pawlak, Simone Berneschi, Wlodzimierz Kutner, Piyush Sindhu Sharma
Summary: The present research reports on the in-water, site-specific photodeposition of glyphosate-containing polyacrylamide nanofilms. The successful photodeposition was confirmed through material characterization and analysis. Additionally, the study found that glyphosate had an impact on polymer gelation. These findings are important for the development of glyphosate-based nanomaterials and chemical sensors.
ACS APPLIED POLYMER MATERIALS
(2022)
Review
Chemistry, Physical
Nabila Yasmeen, Mathieu Etienne, Piyush Sindhu Sharma, Wlodzimierz Kutner
Summary: This review summarizes the recent developments in the molecular imprinting approach for bacteria electrochemical sensing. Designing artificial molecularly imprinted polymer (MIP) and aptamer receptors for bacteria sensing is a challenging task due to the large size and complex cell walls of bacteria. Furthermore, the morphological characterization and recognition mechanism of MIPs are discussed in detail.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Alcina Johnson Sudagar, Shuai Shao, Teresa Zolek, Dorota Maciejewska, Monika Asztemborska, Maciej Cieplak, Piyush Sindhu Sharma, Francis D'Souza, Wlodzimierz Kutner, Krzysztof R. Noworyta
Summary: In this study, we developed a procedure for the selective electro-oxidation of 2,4-dimethylphenol to obtain 3,3',5,5'-tetramethyl-2,2'-biphenol using a molecularly imprinted polymer (MIP) coated electrode. By using density functional theory simulations and spectroscopic techniques, we determined the optimal functional monomers and fabrication conditions for the MIP. The MIP film-coated electrode showed significantly higher selectivity and yield compared to the nonimprinted polymer (NIP) film-coated or bare electrodes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biophysics
Patrycja Lach, Alvaro Garcia-Cruz, Francesco Canfarotta, Alistair Groves, Jakub Kalecki, Dominik Korol, Pawel Borowicz, Kostiantyn Nikiforow, Maciej Cieplak, Wlodzimierz Kutner, Sergey A. Piletsky, Piyush Sindhu Sharma
Summary: Redox-active MIP-Gly NPs were developed by incorporating ferrocenylmethyl methacrylate into the synthesis process, enabling them to selectively determine glyphosate without the need for redox probe. MIP-Gly NPs synthesized using allylamine and squaramide-based monomers exhibited the highest selectivity. The MIP-Gly NPs modified Pt-SPEs were characterized using differential pulse voltammetry and electrochemical impedance spectroscopy, with changes in the oxidation peak of the ferrocenyl moieties serving as the analytical signal. The chemosensor showed a low limit of detection and a wide linear dynamic concentration range for glyphosate, allowing successful detection in spiked river water samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Engineering, Environmental
Jaroslaw Mazuryk, Katarzyna Klepacka, Wlodzimierz Kutner, Piyush Sindhu Sharma
Summary: The article reviews the latest reports on smart sensors for determining glyphosate (GLP), the active agent of GLP-based herbicides. The overuse of herbicides has led to environmental pollution and health issues. Smart sensors, combined with data science and nanosensors, can provide practical tools for personalized diagnostics, real-time monitoring, and site-specific herbicide management.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Mei-Hwa Lee, Cheng-Chih Lin, Wlodzimierz Kutner, James L. Thomas, Chu-Yun Lin, Zofia Iskierko, Chien-Yu Lin, Piyush S. Sharma, Hung-Yin Lin
Summary: A sensitive polymer-coated electrode for measuring MMP-1 expression was developed by incorporating MoS2 nanosheets into peptide-imprinted electrochemical sensors. The response of the sensors was significantly enhanced by MoS2 doping, and the method was successfully applied in measuring MMP-1 expression using a CRISPR/dCas9a system.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jyoti, Teresa Zolek, Dorota Maciejewska, Edyta Gilant, Elzbieta Gniazdowska, Andrzej Kutner, Krzysztof R. Noworyta, Wlodzimierz Kutner
Summary: In this study, we developed a chemical sensor for detecting duloxetine in human plasma. Molecularly imprinted polyacrylic nanoparticles were synthesized and combined with single-walled carbon nanotube scaffolds to form surface constructs. The chemosensor showed a wide linear dynamic concentration range and a low limit of detection using differential pulse voltammetry.