期刊
ANALYTICAL CHEMISTRY
卷 84, 期 6, 页码 2916-2922出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac203488p
关键词
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资金
- U.S. National Institutes of Health [ES16865]
- Department of Energy [DE- FG02-08ER64568]
In this work, two methods with complementary features, catalytic and molecular beacon (CAMB) and label-free fluorescent sensors using an abasic site, have been combined into new label-free CAMB sensors that possess advantages of each method. The label-free method using a dSpacer-containing molecular beacon makes CAMB more cost-effective and less interfering with the catalytic activity, while CAMB allows the label-free method to use true catalytic turnovers for signal amplifications, resulting in a new label-free CAMB sensor for Pb2+ ion, with a detection limit of 3.8 nM while maintaining the same selectivity. Furthermore, by using CAMB to overcome the label-free method's limitation of requiring excess enzyme strands, a new label-free CAMB sensor using aptazyme is also designed to detect adenosine down to 1.4 mu M, with excellent selectivity over other nucleosides.
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