Article
Biochemistry & Molecular Biology
Yongfeng Song, Zhichao Lv, Yue Wang, Chunxiang Li, Yue Jia, Yong Zhu, Mengna Cao, Yu Zhou, Xing Zeng, Zhenhua Wang, Lin Zhang, Hong Di
Summary: Seed storability is an important trait for grain quality and germplasm conservation. This study identified miRNAs and target genes involved in seed storability in maize by miRNA sequencing and analysis. Transcriptome sequencing and RT-PCR confirmed six pairs of differentially expressed miRNAs associated with seed storability, along with their negative target genes. The enrichment analysis suggested that the regulation of seed storability involves ethylene activation signaling pathway, hormone synthesis and signal transduction, and plant organ morphogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Forestry
Guoting Liang, Jing Guo, Shuyong Zhang, Guangcan Zhang
Summary: The study found that allelopathy caused by p-hydroxybenzoic acid (pHBA) affects gene expression in Neva leaves, resulting in changes in genes related to photosynthesis and respiration, as well as degradation of mRNA regulating gene expression during allelopathy.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Forestry
Yuanxun Tao, Chaofeng Li, Ying Liu, Changzheng Xu, Shin Okabe, Norihisa Matsushita, Chunlan Lian
Summary: In this study, high-throughput sequencing of small RNAs and degradome tags from Populus tomentosa roots inoculated with Cenococcum geophilum revealed changes in the accumulation of multiple miRNAs and miRNA-mediated mRNA cleavage during ECM formation. These findings suggest important roles of miRNAs in the regulatory network underlying symbiotic development.
Article
Biotechnology & Applied Microbiology
Jing Liu, Xiaonan Liu, Siju Zhang, Shanshan Liang, Weijiang Luan, Xuan Ma
Summary: TarDB is an online database that collects three categories of relatively high-confidence plant miRNA targets: (i) cross-species conserved miRNA targets; (ii) degradome/PARE sequencing supported miRNA targets; (iii) miRNA-triggered phasiRNA loci. It provides a user-friendly interface for users to easily search, browse, and retrieve miRNA targets and miRNA-triggered phasiRNAs in a broad variety of plants, including lineage-specific or species-specific miRNAs. TarDB serves as a useful web resource for exploring relatively high-confidence miRNA targets and miRNA-triggered phasiRNAs in plants.
Article
Chemistry, Applied
Chen Chen, Lijia Shi, Yuqi Bin, Chenghui Liu, Mixia Tian, Aili Jiang
Summary: This study investigates the molecular mechanism of early browning response in fresh-cut apple and identifies genes related to respiratory, energy, antioxidant, lipid, and secondary metabolism activation in the early stage of browning.
Article
Plant Sciences
Rui Shi, Wei Jiao, Lan Yun, Zhiqiang Zhang, Xiujuan Zhang, Quanzhen Wang, Ying Li, Fugui Mi
Summary: This study identified drought response genes and miRNAs in M. ruthenica under drought and rewatering treatments using transcriptome, small RNA, and degradome sequencing. The findings offer valuable insights for understanding the biological mechanisms of this plant in response to abiotic stress, particularly drought stress.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Immunology
Ibrahim A. Akkouh, Timothy Hughes, Vidar M. Steen, Joel C. Glover, Ole A. Andreassen, Srdjan Djurovic, Attila Szabo
Summary: Despite the high heritability of schizophrenia, the detailed pathophysiology and etiology are still unknown. Recent findings suggest that abnormal inflammatory regulation and miRNAs are involved. Comparative analysis of miRNome in SCZ astrocytes revealed four differentially expressed miRNAs with lower baseline expression compared to healthy controls. Peripheral blood analysis also showed significantly lower expression of specific target genes in SCZ patients, further enhancing the understanding of SCZ pathobiology.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Genetics & Heredity
Junhong Zhang, Yan Lin, Fangmin Wu, Yuting Zhang, Longjun Cheng, Menghui Huang, Zaikang Tong
Summary: The study uncovered the regulatory role of microRNAs in the adaptive responses of Betula luminifera to low phosphate stress and their involvement in combating reactive oxidative species. Through deep sequencing and analysis of transcriptome and small RNAs from different plant parts, the study sheds light on the genetic engineering for high phosphorus use efficiency in forestry trees.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Qian Li, Nadil Shah, Xueqing Zhou, Huiying Wang, Wenlin Yu, Jiajie Luo, Yajun Liu, Genze Li, Chao Liu, Chunyu Zhang, Peng Chen
Summary: The study revealed that miRNAs play different roles in response to Clubroot disease infection in resistant and susceptible plants, potentially participating in the resistance mechanisms through regulating growth, cell death, and metabolic pathways.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
Zhuang Wen, Yi Hong, Zhilang Qiu, Kun Yang, Qiandong Hou, Guang Qiao, Xiaopeng Wen
Summary: Rootstock plays a crucial role in plant growth, but the molecular mechanism of how it regulates scion growth is not well understood. The regulatory roles of miRNAs in sweet cherry grafting remain unclear. Research indicates that miRNAs mediate scion growth through various aspects in the grafting process.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Yingting Zhang, Junjie Yang, Lijuan Zhu, Jinyu Xue, Hailiang Hu, Jiebing Cui, Jin Xu
Summary: This study identified a possible role of miRNA-regulated pathways in needle color in Cryptomeria fortunei, a conifer tree species. A miRNA-target pigment biosynthesis regulatory network was constructed, with miRNA-MYBs confirmed as key regulators of needle discoloration. These findings offer new insight into posttranscriptional regulatory mechanisms in gymnosperms and may contribute to genetic improvement of evergreen C. fortunei needles mediated by miRNAs.
Article
Forestry
Xianqiang Wang, Shu Yao, Win Pa Pa Myo Htet, Yuchen Yue, Zhuanzhuan Zhang, Kuan Sun, Sijie Chen, Keming Luo, Di Fan, Isabel Allona
Summary: This study reveals the intricate regulatory mechanism of lignin biosynthesis in Populus tomentosa Carr., showing the interaction between miR828 and MYB171, MYB011 in regulating lignin synthesis through both transcriptional and post-transcriptional modulation.
Article
Biochemistry & Molecular Biology
Peng Li, Jiaxuan Zhou, Dan Wang, Lianzheng Li, Liang Xiao, Mingyang Quan, Wenjie Lu, Liangchen Yao, Yuanyuan Fang, Chenfei Lv, Fangyuan Song, Qingzhang Du, Deqiang Zhang
Summary: The study conducted genome-wide analysis on a large number of unrelated P. tomentosa accessions, identifying significant Single Nucleotide Polymorphisms (SNPs) associated with stem lenticel traits and discovering key genes involved in lenticel formation. The findings revealed distinct selection signatures of causative genes in different climate regions, and demonstrated the roles of PtoNAC083 and PtoMYB46 genes in cell division and expansion during lenticel formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Tiantian Ye, Xu Huang, Tianxiao Ma, Ying Li, Xiaofeng Wang, Hai Lu, Hua Xue
Summary: After maturity, seed vigor irreversibly decreases. This study conducted a multi-omics analysis to investigate the regulation of seed aging in elm seeds. The results identified differentially expressed genes and miRNAs, as well as miRNA-target gene pairs during seed aging. The degradome data revealed the exact degradation sites of certain miRNAs on target genes.
Article
Plant Sciences
Abhishek Bohra, Prasad Gandham, Abhishek Rathore, Vivek Thakur, Rachit K. Saxena, S. J. Satheesh Naik, Rajeev K. Varshney, Narendra P. Singh
Summary: The comparative analysis of genome-wide miRNAs and their gene targets between cytoplasmic male sterile (CMS) and fertile lines of pigeonpea suggests a possible role of miRNA-regulated pathways in reproductive development.