Article
Biochemical Research Methods
Bin Liu, Yinghua Yan, Hongze Liang, Keqi Tang, Chuan-Fan Ding
Summary: Researchers developed a green strategy to prepare carbonaceous spheres rich in phosphoric acid groups with D-Glucose 6-phosphate sodium salt as a sole carbon source. The carbonaceous spheres were further functionalized with Ti4+ to obtain excellent performance in selective capture of phosphopeptides. The CS-Ti4+ showed great potential in mass spectrometry-based phosphoproteome studies by capturing low-abundance phosphopeptides from human saliva.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Bin Liu, Baichun Wang, Yinghua Yan, Keqi Tang, Chuan-Fan Ding
Summary: The Fe3O4@mSiO(2)@APA@Ti4+/Nb5+ material has shown promising potential in effectively enriching global phosphopeptides, with high selectivity, low detection limit, good reproducibility, and excellent loading capacity.
Article
Chemistry, Analytical
Ying Chen, Bin Liu, Zhengbo Chen, Xia Zuo
Summary: Enrichment techniques for phosphopeptides often involve interactions between phosphate groups and metal ions or metal oxides. In this study, an electrochemical sensor based on TiO2 NPs was designed to detect phosphopeptides in protein samples pretreated with AuNPs, showing high sensitivity and a wide linear concentration range. Additionally, TiO2 and GO composite materials were synthesized to enhance the detection capability, with the uniformly dispersed anatase crystal TiO2 and GO composite-modified electrode exhibiting a lower detection limit.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Sen Zhang, Jia-yuan Li, Wei Gao, Jun-qin Qiao, Hong-zhen Lian
Summary: Ti3C2-Fe3O4 composites with exposed non-modified Ti3C2 MXene nanosheets were designed and prepared for the enrichment of phosphopeptides. The composites interacted with the phosphopeptides through metal oxide affinity chromatography and hydrophilic interaction chromatography mechanisms. The magnetic nanomaterial showed high sensitivity and selectivity, and satisfactory results were obtained in the enrichment of phosphopeptides from real samples and traditional Chinese medicine injection.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Jiani Wu, Xueting Jin, Canhong Zhu, Yinghua Yan, Chuan-Fan Ding, Keqi Tang
Summary: A novel zwitterionic hydrophilic metal-organic framework (MOF)-functionalized material, PEI-ZIF-8@Au@GSH, demonstrated excellent performance in capturing glycopeptides with high sensitivity and outstanding enrichment selectivity by grafting GSH and Au onto the base material, PEI-ZIF-8.
Article
Chemistry, Analytical
Qianying Sheng, Chenli Xue, Yang Zhou, Junyan Li, Huihui Yuan, Yanxiong Ke, Minbo Lan
Summary: The study demonstrated the selective enrichment of glycopeptides and phosphopeptides through the preparation of Al3+-doping-TiO2 mixed oxide microspheres, leading to significantly improved signal-to-noise ratio in the samples of human serum immunoglobulin G (IgG) and alpha-casein. This method provides a novel insight for post-translational modification proteomics research.
Article
Chemistry, Multidisciplinary
Cheng Chen, Xiaofei Zhang, Xuefang Dong, Han Zhou, Xiuling Li, Xinmiao Liang
Summary: Protein glycosylation and phosphorylation play important roles in cellular processes and disease pathology, with strategies developed to enrich and analyze these peptides simultaneously. A novel strategy using TiO2 for enrichment, online deglycosylation, and subsequent elution of phosphopeptides was developed, resulting in significantly increased numbers of glycopeptides and phosphopeptides identified in mouse brain samples. This study expands the application of TiO2 and may have implications for monitoring multiple post-translational modifications in proteins.
FRONTIERS IN CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Lingzhu Yu, Bin Luo, Xiaoxi Zhou, Yicheng Liu, Fang Lan, Yao Wu
Summary: Efficient enrichment of phosphopeptides is crucial for phosphoproteomics research, and the use of MXene-derived materials, such as Fe3O4/TiO2@Ti3C2Tx composites, presents a promising strategy with high sensitivity, selectivity, repeatability, and enrichment capacity. This work demonstrates the potential of MXene composites in biomedical applications for phosphoproteome analysis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Hongwei Wang, Ruizhi Tang, Shicong Jia, Shujuan Ma, Bolin Gong, Junjie Ou
Summary: This paper introduces a novel monodisperse MAR microsphere (Ti4+-Brush@MAR) prepared and modified via surface-initiated atom transfer radical polymerization (SI-ATRP), showing high enrichment specificity for phosphopeptides in beta-casein or milk digest samples.
Article
Chemistry, Analytical
Dandan Jiang, Siyu Wu, Siqi Lv, Ruixue Qi, Yangyang Li, Jinghai Liu
Summary: A new chelator, L-Alanyl-L-Glutamine, was used to prepare immobilized magnetic graphite nitride material with cerium ions. The resulting nanocomposite showed high surface area, hydrophilicity, positive electricity, and magnetic response. As an immobilized metal ion affinity chromatography sorbent, it exhibited high sensitivity, selectivity, and recyclability. The analysis of A549 cell lysate identified numerous phosphopeptides mapped to phosphoproteins.
Article
Chemistry, Analytical
Yanting He, Qiong Zheng, Zian Lin
Summary: The Ti4+-immobilized hierarchically porous zirconium-organic frameworks (THZr-MOFs) showed high adsorption capacity and enrichment selectivity for phosphopeptides, and demonstrated great potential in analyzing complex samples.
Article
Chemistry, Multidisciplinary
Antonin Lavigne, Thomas Gehin, Benoit Gilquin, Vincent Jousseaume, Marc Veillerot, Claude Botella, Celine Chevalier, Cecile Jamois, Yann Chevolot, Magali Phaner-Goutorbe, Christelle Yeromonahos
Summary: This study demonstrates that using chemically functionalized nanoporous silicon surfaces can address the limitations of MALDI-ToF MS analysis. The physicochemical properties of metabolites and surfaces play a significant role in MS analysis. Porous substrates are more suitable for hydrophobic metabolites, while plane substrates are better for hydrophilic metabolites.
Article
Biochemical Research Methods
Xiaoya Zhang, Quanshou Feng, Zehu Xie, Fuxing Xu, Yinghua Yan, Chuanfan Ding
Summary: In this study, a new type of covalent organic framework material was successfully prepared for the enrichment of phosphorylated peptides and exosomes using an immobilized metal ion affinity chromatography strategy. The material showed high selectivity and sensitivity in practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Azhar Irfan, Wenxia Feng, Kexin Liu, Khan Habib, Qishu Qu, Li Yang
Summary: Phosphorylation, an important post-translational modification of proteins, plays a key role in regulating biological reactions. Due to the low abundance of phosphopeptides and limitations in ionization efficiency in mass spectroscopic detection, efficient and selective enrichment methods are crucial. The developed CSMS@TiO2 nanocomposites show promising results in enriching phosphopeptides and excluding massive proteins, enhancing sensitivity for identifying phosphopeptides.
Article
Chemistry, Analytical
Yuyan Xue, Haimei Shi, Bin Feng, Liang Qiao, Chuanfan Ding, Shaoning Yu
Summary: In this study, bacteria in blood culture bottles were enriched using co-magnetic beads for MALDI-TOF MS profiling. Compared to single magnetic beads, co-magnetic beads showed better bacterial capture efficiency, decreasing false-negative results. This proposed strategy is more suitable for enriching clinically unknown bacteria from blood culture bottles for MALDI-TOF MS database identification.
