期刊
ANALYTICAL CHEMISTRY
卷 83, 期 8, 页码 2862-2865出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac200452b
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资金
- NSF [DBI 092351]
- Science Foundation Arizona
- [NIH72623]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0923551] Funding Source: National Science Foundation
As scientists begin to appreciate the extent to which quaternary structure facilitates protein function, determination of the subunit arrangement within noncovalent protein complexes is increasingly important. While native mass spectrometry shows promise for the study of noncovalent complexes, few developments have been made toward the determination of subunit architecture, and no mass spectrometry activation method yields complete topology information. Here, we illustrate the surface-induced dissociation of a heterohexamer, toyocamycin nitrile hydratase, directly into its constituent trimers. We propose that the single-step nature of this activation in combination with high energy deposition allows for dissociation prior to significant unfolding or other large-scale rearrangement This method can potentially allow for dissociation of a protein complex into subcomplexes, facilitating the mapping of subunit contacts and thus determination of quaternary structure of protein complexes.
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