期刊
ANALYTICAL CHEMISTRY
卷 83, 期 22, 页码 8802-8809出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac201676a
关键词
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资金
- National Key Program for Basic Research of China [2011CB910603, 2012CB910603]
- National Natural Science Foundation of China [20735005, 20905077, 30900258, 31100591]
- International Scientific Cooperation Project of China [2011DFB30370]
- National Key Laboratory Foundation of China [SKLP-Y200902]
Glycosylation modifications of proteins have been attracting increasing attention due to their roles in the physiological and pathological processes of the cell. Core fucosylation (CF), one special type of glycan structure in glycoproteins, has been linked with tumorigenesis. The study of protein glycosylation has been hindered by the technical challenges caused by the microheterogeneity of glycan modifications. In commonly used methods, sugar chains on the peptide were released using endoglycosidase, and the glycan and peptides were analyzed separately with mass spectrometry. Although mass spectrometric analysis can be performed easily in this way, an increase in false positives when assigning glycosites was inevitable. Our earlier research demonstrated a strategy combining Endo F3-catalyzed partial deglycosylation with MS3 (MS/MS/MS) scanning triggered by the neutral loss of a fucose to precisely identify CF proteins on a large scale. In this research, fragmentations of partially deglycosylated glycopeptides were studied using a triple quadrupole mass spectrometer, and a quantification method that coupled our published identification strategy with multiple reaction monitoring-mass spectrometry (MRM-MS) analysis was developed to obtain site-specific quantification information of core fucosylated peptides. To illustrate the feasibility. of the quantification method, the CF peptides of target proteins in clinical serum were quantified and compared as a preliminary demonstration.
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