Article
Nanoscience & Nanotechnology
Sahar Cheraghi Shahi, Mehdi Dadmehr, Behnaz Korouzhdehi, Abolfazl Tavassoli
Summary: A colorimetric competitive assay for detecting AFB1 in foods was developed based on the interaction of AuNPs@gelatin. The method showed a linear detection range of 10 to 140 pg ml(-1), with a detection limit of 4 pg ml(-1) and recoveries percentage of 92.4%-95.3% in real saffron samples. This approach offers a simple, cost effective, and specific method for AFB1 toxin detection in food samples.
Article
Chemistry, Analytical
Yue He, Jing Zhou, Ruijie Fu, Yanlin Liu, Yiwen Wang, Haoran Liu, Jing Zhao, Yongliang Cui, Bining Jiao
Summary: An ultrasensitive colorimetric method for detecting genomic DNA of Alternaria from citrus fruit samples was described in this work, utilizing a system of five groups of reporter probes that form a DNA-heptamer sandwich structure on magnetic beads. The method has a low detection limit and is suitable for Alternaria genetic detection with its high sensitivity.
Article
Chemistry, Analytical
Qingcai Yu, Jing Zhang, Wanwei Qiu, Kun Li, Lisheng Qian, Xueji Zhang, Guodong Liu
Summary: A gold nanorod-based lateral flow nucleic acid biosensor was developed for visual detection of DNA with high sensitivity and short test time. By forming a sandwich-type complex on the LFNAB, target DNA could be detected without instrumentation, and quantitative analysis was achieved using a portable strip reader.
Article
Biotechnology & Applied Microbiology
Fernanda Raquel Wust Schmitz, Karina Cesca, Alexsandra Valerio, Debora de Oliveira, Dachamir Hotza
Summary: Novel rapid methodologies involving colorimetric biosensors and gold nanoparticles have been developed for the detection of bacteria, specifically Pseudomonas aeruginosa. The biosensor relies on the color change of gold nanoparticles induced by NaCl after incubation with bacteria and aptamer binding. The optimized conditions for detection were found to be 5 μM aptamer concentration and 200 mM of NaCl. The detection limit for P. aeruginosa was 10(5) CFU mL(-1) by the naked eye and 10(4) CFU mL(-1) by UV-Vis spectrometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Xiangyu Song, Huhu Wang, Xinglian Xu
Summary: In this study, a novel biosensing strategy for bacterial sensing based on modified gold nanoparticles (AuNPs) combined with amikacin as the molecular recognition element is proposed. The biosensor shows a good linear correlation with bacterial concentration and can detect bacteria within a short period of time without the need for expensive reagents or complex instruments.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Monisha Elumalai, Andrey Ipatov, Joana Carvalho, Joana Guerreiro, Marta Prado
Summary: A new DNA target amplification strategy combined with dual-mode colorimetric naked eye and smartphone analysis is proposed for the rapid and sensitive detection of zebra mussel environmental DNA. This method demonstrates high specificity and sensitivity, suitable for field analysis of environmental samples without the need for expensive laboratory equipment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Sara Kooti, Sepide Kadivarian, Ramin Abiri, Parviz Mohajeri, Sara Atashi, Hossein Ahmadpor, Amirhooshang Alvandi
Summary: This study aimed to develop a modified gold nanoparticle (AuNP) colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis (MTB) in sputum specimens. The biosensor showed 100% sensitivity and specificity in detecting total sputum DNA in less than 15 min. With promising diagnostic value indices, this method provides an affordable, rapid, reliable, and cost-beneficial approach for direct MTB detection in sputum specimens.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Subash C. B. Gopinath, Santheraleka Ramanathan, Suresh Chinni, Vicneswarry Dorairaj, Thangavel Lakshmipriya
Summary: Nonprotein coding RNA (npcRNA) is a transcribed gene sequence that cannot translate into protein but plays a specific role in modulation and regulation mechanisms. This study developed a highly specific and sensitive chromatographic assay using gold nanoparticles (AuNPs) to detect the presence of the npcRNA Sau-02 gene, which is specifically present in Staphylococcus aureus and methicillin-resistant S. aureus. The hybridization between Sau-02 npcRNA gene sequences was detected through the aggregation of AuNPs in salt solution, in the presence of probe-target gene sequences.
SCIENTIFIC REPORTS
(2022)
Article
Spectroscopy
Shuyan Xiao, Jiping Lu, Liang Sun, Shengli An
Summary: A new colorimetric biosensor for specific detection of azlocillin was developed using DNA aptamer and unmodified gold nanoparticles. The biosensor showed a red solution in the absence of azlocillin and a color change from red to blue in the presence of azlocillin. The biosensor exhibited a linear relationship with a detection limit of 11.6 nM and was successfully used to detect azlocillin in milk and tap water samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Chie Kojima, Hai Xia, Yojiro Yamamoto, Hiroshi Shiigi
Summary: In this study, a naked-eye colorimetric sensor with dual pH and temperature sensitivity was developed using gold nanoparticles and polyamidoamine dendrimers.
Article
Nanoscience & Nanotechnology
Yawen He, Zhiyuan Xu, Tom Kasputis, Xue Zhao, Itati Ibanez, Florencia Pavan, Marina Bok, Juan Pablo Malito, Viviana Parreno, Lijuan Yuan, R. Clay Wright, Juhong Chen
Summary: A whole-cell biosensor with nanobody-displayed yeasts and gold nanoparticles as signal transductions was developed for colorimetric detection of SARS-CoV-2 spike proteins. The biosensor showed high sensitivity, with a linear detection range from 0.01 to 1 μg/mL and a limit of detection of 0.037 μg/mL. It was also capable of detecting spike proteins from different SARS-CoV-2 variants in human serum, providing potential for variant detection.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Inorganic & Nuclear
Selcan Karakus, Guelsen Baytemir, Cemal Ozeroglu, Nevin Tasaltin
Summary: In this study, a smartphone-integrated colorimetric biosensor was developed for monitoring the concentration of ammonia in food safety. The biosensor showed high selectivity, a wide concentration range, and a low detection limit.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Vanessa Thoeny, Eva Melnik, Melanie Huetter, Malahat Asadi, Pooyan Mehrabi, Thomas Schalkhammer, Walter Pulverer, Thomas Maier, Giorgio C. Mutinati, Peter Lieberzeit, Rainer Hainberger
Summary: As personalized anticancer therapy continues to evolve, mutation screening is becoming increasingly important. This study developed an alternative detection strategy for fast genetic diagnostics at the point of care, specifically for detecting cancer-associated point mutations in the PIK3CA gene in breast cancer.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Zou, Chuoying Mai, Mengyan Li, Yufen Lai
Summary: In this study, a pH-responsive gold nanoparticle assembly (RGA) assisted by i-motif DNA was developed for highly sensitive and reversible response to pH changes between 6.20 and 7.40. The acidic pH triggers disassembly of RGA, changing the color from blue-purple to red, allowing pH estimation by naked-eye determination or UVevis spectroscopy. Furthermore, the RGA can detect slight pH fluctuations in catalytic reactions, providing a new strategy for on-site and real-time pH sensing.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Dongfei Chen, Yanfang Wu, Richard D. Tilley, J. Justin Gooding
Summary: An ultra-sensitive and highly-selective electrochemical biosensor has been developed for rapid detection of DNA methylation in blood, providing a promising method for minimally invasive diagnosis of ovarian cancer.
BIOSENSORS & BIOELECTRONICS
(2022)