Review
Materials Science, Multidisciplinary
Xianyang Zhang, Pengfei Chen, Siwuxie He, Bowen Jiang, Yong Wang, Yonghua Cheng, Jian Peng, Francis Verpoort, John Wang, Zongkui Kou
Summary: Biosensors featuring single molecule detection offer great opportunities in various fields, but face challenges due to the lack of activity, precision molecule selectivity, and understanding of the operating mechanism. Single-atom catalysts (SACs), particularly those that mimic the natural metalloenzyme structure, provide practical-use feasibilities for single molecule detections with high molecular selectivity and easy fabrication. This review discusses the history, advantages, and applications of SACs in molecule-scale biosensors, emphasizing their sensing modes and coordination-modulated signal amplifications.
Article
Chemistry, Analytical
Jeongwoo Ham, Byung Ju Yun, Won-Gun Koh
Summary: In this study, shape-coded suspension arrays were developed for SERS-based multiplex biosensing using different shapes of PEG-based hydrogel microparticles. Good performance was demonstrated in detecting glucose and HSA.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Antonio Carone, Pablo Mariani, Anthony Desert, Marco Romanelli, Jacopo Marcheselli, Marco Garavelli, Stefano Corni, Ivan Rivalta, Stephane Parola
Summary: Chiral plasmonic nanomaterials have promising applications. This study expands the range of available chiral plasmonic nanostructures and proposes the importance of the outer part of the nanoparticles shell on plasmonic circular dichroism.
Article
Chemistry, Analytical
Siqi Gao, Renbin Zhou, Soham Samanta, Junle Qu, Tymish Y. Ohulchanskyy
Summary: This review summarizes recent progress in the development of various plasmon-enhanced luminescence (PEL)-based biosensors and bioimaging platforms for diverse biological and biomedical applications. The study evaluates the performance improvement of PEL-based biosensors in efficiently detecting biomarkers (proteins and nucleic acids) in point-of-care tests and discusses the pros and cons of recently developed PEL-based biosensing platforms on substrates or in solutions. It also includes a brief discussion on integrating PEL-based biosensing platforms into microfluidic devices as a promising multi-responsive detection method. The review presents comprehensive details about the recent advances in the development of various PEL-based multi-functional (passive targeting, active targeting, and stimuli-responsive) bioimaging probes, highlighting the scope of future improvements in devising robust PEL-based nanosystems to achieve more effective diagnostic and therapeutic insights by enabling imaging-guided therapy.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Lin Wei, Zhongju Ye, Chen Zhang, Hua Liu, Jie Yuan, Lehui Xiao
Summary: The goal of developing sensing methods for biomolecular assay is to provide new tools for discerning trace molecules in complex biological environments with high sensitivity and specificity. Single-molecule detection allows for resolution of small differences between ostensibly identical samples that cannot be achieved with ensemble measurements. Efforts have been made on exploiting single-molecule detection methods for early disease diagnosis. In this review, recent achievements in single-molecule detection with optical microscopy are summarized, including statistical single-molecule counting with total internal reflection fluorescence (TIRF), spectrum-resolved single-molecule/particle imaging for multiplex assay, and label-free scattering detection in an interference mode for virus or protein counting. The mechanisms, advantages, challenges, and prospects of adopting optical microscopy for single-molecule detection are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fengzhou Xu, Taiping Qing, Zhihe Qing
Summary: Fluorescent metal nanoclusters (MNCs) possess excellent fluorescence properties and are widely used in biochemical analysis and bioimaging.
Article
Chemistry, Analytical
Edwin J. Ortiz-Riano, Diana L. Mancera-Zapata, Martha Ulloa-Ramirez, Fernando Arce-Vega, Eden Morales-Narvaez
Summary: Macromolecular association is important in biomedical sciences. This study presents a real-time biosensing platform using graphene oxide-modified microwell plates and fluorophore-labeled proteins to determine protein-binding constants. The platform proves to be comparable and consistent with existing methods in determining protein kinetics constants.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hajir Hilal, Qiang Zhao, Jeongwon Kim, Sungwoo Lee, MohammadNavid Haddadnezhad, Sungjae Yoo, Soohyun Lee, Woongkyu Park, Woocheol Park, Jaewon Lee, Joong Wook Lee, Insub Jung, Sungho Park
Summary: This study demonstrates a new three-dimensional nanostructure with polarization-independent SERS activity, prepared through a precise synthesis method. This unique structure shows efficient interaction with light and analytes, providing new possibilities for optical-based sensing.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Lei-Lei Nian, Tao Wang, Jing-Tao Lu
Summary: This study theoretically predicts that the emitted light from the plasmon mode in an STM single molecular junction can be squeezed with reduced quantum fluctuations under external laser drive. By selectively exciting energy levels, the squeezed plasmon can exhibit sub-or superPoissonian statistical properties. It is also demonstrated that the molecular excitonic mode can be squeezed simultaneously.
Article
Chemistry, Multidisciplinary
Izabela Kaminska, Johann Bohlen, Renukka Yaadav, Patrick Schueler, Mario Raab, Tim Schroeder, Jonas Zaehringer, Karolina Zielonka, Stefan Krause, Philip Tinnefeld
Summary: Graphene is a game-changing material with unique mechanical and electrical properties. This study highlights its application as a transparent yet fluorescence-quenching energy transfer acceptor, showcasing its versatility in various fields such as molecular height determination, DNA structure visualization, and orientation determination of energy transfer pairs.
ADVANCED MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Chia-Wen Kuo, Sheng-Hann Wang, Shu-Cheng Lo, Wei-Han Yong, Ya-Lun Ho, Jean-Jacques Delaunay, Wan-Shao Tsai, Pei-Kuen Wei
Summary: The study proposes a method for sensitive detection of small molecules by coupling image dipoles of gold nanoparticles with Fano resonance of capped gold nanoslits. The coupling mechanism was verified through calculations and experiments, showing potential for detecting low concentrations of small molecules. This resonant coupling significantly pushes the sensitivity boundary, with the detection limit reported to be 3 orders of magnitude lower than prism-based SPR.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Multidisciplinary
Gour Mohan Das, Stefano Manago, Maria Mangini, Anna Chiara De Luca
Summary: Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for biosensing applications due to its high sensitivity and multiplex detection capabilities. This review outlines the key aspects of SERS in the biomedical field, including mechanisms, substrates and fabrication methods, performance characterization factors, and applications.
Article
Chemistry, Inorganic & Nuclear
XueQian Xiao, Xiao Hu, Qiming Liu, Yuling Zhang, Guo-Jun Zhang, Shaowei Chen
Summary: Single-atom nanozymes (SAzymes) have emerged as promising alternatives to natural enzymes due to their intrinsic enzyme-like properties and excellent stability. They overcome the major obstacles of low active site density and unclear crystal structure in traditional nanozymes. The well-defined coordination structures and homogeneously dispersed active sites of SAzymes provide rare pathways to investigate catalytic mechanisms. In this review, we summarize the latest progress in the rational design and engineering of SAzymes, as well as their applications in biomedicine and biosensing. We also highlight the remaining challenges and future perspectives of this emerging technology.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Engineering, Biomedical
Drew A. Hall, Nagaraj Ananthapadmanabhan, Chulmin Choi, Le Zheng, Paul P. P. Pan, Christoph Von Jutrzenka, Thuan Nguyen, Jose Rizo, Macklan Weinstein, Raymond Lobaton, Prem Sinha, Trevor Sauerbrey, Cruz Sigala, Kathryne Bailey, Paul J. Mudondo, Ashesh Ray Chaudhuri, Simone Severi, Carl W. Fuller, James M. Tour, Sungho Jin, Paul W. Mola, Barry Merriman
Summary: This work presents the first CMOS molecular electronics chip that allows direct, real-time observation of molecular interaction kinetics. It provides a new platform for applications in biological research, diagnostics, drug discovery, and more.
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2022)
Article
Chemistry, Multidisciplinary
Saisai Yuan, Xiaohui Xu, Abdalghani Daaoub, Chao Fang, Wenqiang Cao, Hang Chen, Sara Sangtarash, Jiangwei Zhang, Hatef Sadeghi, Wenjing Hong
Summary: Single-atom control allows for modulation of electrical conductance and thermopower in polyoxometalate clusters, leading to manipulation of charge transport at the single-atom level. This work provides fundamental insight into the tuning of charge transport in POM single-cluster junctions through atomic precision.