Article
Chemistry, Analytical
Ludwig R. Sinn, Sven H. Giese, Marchel Stuiver, Juri Rappsilber
Summary: Ion-mobility spectrometry has the potential to solve analytically challenging research questions by adding an extra separation dimension to liquid chromatography-mass spectrometry. By using machine learning, the understanding of field asymmetric waveform ion-mobility spectrometry for the analysis of cross-linked peptides has been deepened and ideal compensation voltages correlating with chromatography fraction number have been identified.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Haixia Zhang, Randy W. Purves, Thomas D. Warkentin, Albert Vandenberg
Summary: Faba bean seeds are a rich source of proteins and levodopa, but the presence of vicine and convicine limits its consumption. A quantification method using FAIMS and QqQ was previously reported, and a similar method with commercial instruments was developed. Both methods provided accurate measurements and recovery, but the commercial method had a lower limit of quantification and a wider linear range. The results showed that levodopa levels in faba bean were genotype dependent, but not directly correlated with vicine or convicine levels.
Article
Biochemical Research Methods
Jacob W. McCabe, Benjamin J. Jones, Thomas E. Walker, Robert L. Schrader, Adam P. Huntley, Jixing Lyu, Nathan M. Hoffman, Gordon A. Anderson, Peter T. A. Reilly, Arthur Laganowsky, Vicki H. Wysocki, David H. Russell
Summary: This study presents a digital-waveform dual-quadrupole mass spectrometer to improve the performance of a drift tube FT-IMS high-resolution Orbitrap mass spectrometer in studying large proteins and protein complexes. The dual-quadrupole analyzer allows for low-energy collisional activation of ions and precise mass selection using digital-waveform technology, enhancing the capabilities of the instrument at a lower cost compared to traditional RF drivers.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Ying Shi, Huang-Fei Jin, Min-Zhen Shi, Jun Cao, Li-Hong Ye
Summary: In this study, a miniaturized solid phase extraction method and supercritical fluid chromatography combined with ion mobility quadrupole time-of-flight mass spectrometry were used for the qualitative and quantitative analysis of carbamate pesticide residues. The method was validated and applied successfully to the enrichment and detection of carbamate residues in ginger samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Yinghao Wang, Xianxie Zhang, Wenya Hu, Chenglong Dong, Dali Fu, Sara Girmay Habtegabir, Yehua Han
Summary: An ultra-fast screening method was developed for the analysis of free fatty acids using trapped ion mobility spectrometry coupled with mass spectrometry. The method allows for the efficient characterization and quantification of free fatty acids, and has been validated for profiling free fatty acids in human plasma.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Minna Juvonen, Edwin Bakx, Henk Schols, Maija Tenkanen
Summary: This study investigated the potential of using TWIMS and CID-TWIMS-MS/MS to separate cereal-derived isomeric arabinoxylanoligosaccharides (A)XOS. The triand pentasaccharide isomers were distinguishable by the ATDs of the precursor ions, while CID-TWIMS-MS/MS could effectively separate isomeric fragment ions produced from tetra- and pentasaccharide (A)XOS. The base peak mobility spectrum was also introduced as a practical tool for (A)XOS fingerprinting.
Article
Chemistry, Multidisciplinary
Karen E. Butler, Yoshihiko Takinami, Adam Rainczuk, Erin S. Baker, Blaine R. Roberts
Summary: Native mass spectrometry combined with IMS-MS technology can be used to study the tertiary and quaternary structure of proteins, validate other structural determination methods, and provide more comprehensive structural information.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jan Leipert, Philipp T. Kaulich, Max K. Steinbach, Britta Steer, Konrad Winkels, Christine Blurton, Matthias Leippe, Andreas Tholey
Summary: Researchers have developed a sample preparation method based on protein aggregation and acidic elution, allowing for sensitive protein identification analysis of single nematodes on a digital microfluidics device. Compared to in-tube sample preparation, this method increases proteoform identifications by 46%. Label-free quantification also reveals changes in proteoform abundance that cannot be distinguished by bottom-up proteomics. This workflow facilitates proteoform-focused analysis on limited availability samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Tlou T. Mosekiemang, Maria A. Stander, Andre de Villiers
Summary: The presence of pharmaceutical compounds in the aquatic environment is a significant environmental health concern, especially with recent evidence of drug metabolites contributing to the overall pharmaceutical load. This study focused on investigating the occurrence of antiretroviral drug metabolites in wastewater samples from South Africa using advanced analytical techniques to separate and identify these complex compounds.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Katie M. Kohoutek, Peter de B. Harrington
Summary: Electrospray ionization ion mobility mass spectrometry (ESI-IMS-MS) is a rapidly progressing analytical technique that examines complex compounds in the gas phase. It separates isomers, provides structural information, and quantitatively identifies various compounds in biological samples. ESI-IMS-MS has wide applications in pharmaceutical, environmental, and manufacturing industries.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zeng Hong-Da, Du Xiao-Xia, Pan Jia-Kai, Wang Yi-Fei, Chen Zhen-Cheng, Li Hua
Summary: A hollow needle-ring ion source was proposed in this study to improve the resolving power and sensitivity of FAIMS systems by introducing helium and argon gases. The experiment showed that helium was more effective than argon in enhancing both parameters, and a mixture of three gases resulted in multiple ion peaks in the FAIMS spectrum.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Thomas E. Walker, Arthur Laganowsky, David H. Russell
Summary: Charge reduction reactions play a crucial role in native mass spectrometry (nMS) by retaining native-like structures and noncovalent interactions of protein complexes. In this study, we provide new evidence that surface-active charge reducing reagents remove excess charge from droplets, thereby decreasing their overall charge. These processes are linked to the charge residue and ion evaporation models of electrospray ionization (ESI), and higher surface activity of amines leads to more efficient charge reduction of protein ions. Additionally, higher emitter potentials in ESI are associated with lower average charge states of protein complexes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jake Breen, Mahin Hashemihedeshi, Roshanak Amiri, Frank L. Dorman, Karl J. Jobst
Summary: Gas chromatography multiplexed with cyclic ion mobility mass spectrometry is a comprehensive two-dimensional separation technique that can resolve compounds that would otherwise coelute in a single-dimension separation. The present study demonstrates a method to mitigate wrap-around in cyclic ion mobility, allowing for non-targeted interpretation of experiments while maintaining the benefits of peak capacities.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Caitlyn Da Costa, Matthew Turner, James C. Reynolds, Samuel Whitmarsh, Tom Lynch, Cohn S. Creaser
ANALYTICAL CHEMISTRY
(2016)
Article
Chemistry, Analytical
Aditya Malkar, Emma Wilson, Tim Harrrison, Dominick Shaw, Colin Creaser
ANALYTICAL METHODS
(2016)
Article
Biochemical Research Methods
P. Perez-Hurtado, E. Palmer, T. Owen, C. Aldcroft, M. H. Allen, J. Jones, C. S. Creaser, M. R. Lindley, M. A. Turner, J. C. Reynolds
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2017)
Article
Biochemical Research Methods
James W. Howard, Richard G. Kay, Ben Jones, Jaimini Cegla, Tricia Tan, Steve Bloom, Colin S. Creaser
Article
Medical Laboratory Technology
Jaimini Cegla, Ben J. Jones, James Howard, Richard Kay, Colin S. Creaser, Stephen R. Bloom, Tricia M. Tan
ANNALS OF CLINICAL BIOCHEMISTRY
(2017)
Article
Energy & Fuels
Katarzyna M. Szykula, Christianne Wicking, Samuel Whitmarsh, Colin S. Creaser, James C. Reynolds
Article
Biochemical Research Methods
Katarzyna M. Szykula, Joris Meurs, Matthew A. Turner, Cohn S. Creaser, James C. Reynolds
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Stephanie Rankin-Turner, Matthew A. Turner, Paul F. Kelly, Roberto S. P. King, James C. Reynolds
Article
Spectroscopy
Kayleigh L. Arthur, Lynsey S. Wilson, Matthew A. Turner, Martin R. Lindley, James C. Reynolds, Colin S. Creaser
INTERNATIONAL JOURNAL FOR ION MOBILITY SPECTROMETRY
(2018)
Article
Chemistry, Analytical
Kayleigh L. Arthur, Matthew A. Turner, Alan D. Brailsford, Andrew T. Kiernan, David A. Cowan, James C. Reynolds, Colin S. Creaserje
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Kayleigh L. Arthur, Matthew A. Turner, James C. Reynolds, Colin S. Creaser
ANALYTICAL CHEMISTRY
(2017)