期刊
ANALYTICAL CHEMISTRY
卷 82, 期 7, 页码 3002-3006出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac1000652
关键词
-
资金
- National Science Foundation of China [0945001, 20890020, 20890022, 2009ZX10605, 90813032]
- Human Frontier Science Program
- Chinese Academy of Sciences [KJCX2-YW-M15]
- Ministry of Science Technology [2007CB714502, 2009CB930001, 2009ZX10004-505]
This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an optic sensor; we call this system micro-LAMP (mu LAMP). It is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. The assay is straightforward in manipulation. It requires a sample volume of 0.4 mu L and is complete within 1 h. The sensitivity of the assay is comparable to standard methods, where 10 fg of DNA sample could be detected under isothermal conditions (63 degrees C). A real time quantitative mu LAMP assay using absorbance detection is possible by integration of optical fibers within the chip.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据