Article
Multidisciplinary Sciences
Serife Ustuner, Mark A. Lindsay, Pedro Estrela
Summary: A promising electrochemical detection method has been developed for detecting miRNAs in diluted serum with a detection limit of 0.38 fM. The accuracy and reliability of the sensors are further improved by using locked nucleic acids-modified magnetic beads. Interferences from other nucleotides or biomolecules in the sample are eliminated effectively in this approach.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Feng Qiu, Xiaoyan Gan, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: This study presents a novel method for sensitive detection of thrombin in human serums. By utilizing target-promoted magnetic nanoparticle-decorated DNA polymers for proximity binding hybridization chain reaction, the detection of thrombin is significantly enhanced.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Hongguo Wei, Shengjun Bu, Ze Wang, Hongyu Zhou, Xue Li, Jiaqi Wei, Xiuxia He, Jiayu Wan
Summary: This study introduced CRISPR-Cas12a and click chemistry-mediated exponential amplification reaction into an electrochemical biosensor for sensitive detection of miRNA-21. The proposed biosensor showed a low detection limit and has potential applications in point-of-care testing and field molecular diagnostics.
Article
Chemistry, Multidisciplinary
Jing Zhang, Meifeng Hou, Guanyu Chen, Huifang Mao, Wenqian Chen, Wenshen Wang, Jinghua Chen
Summary: An electrochemical biosensor based on DNA nano-bridge was developed for detecting exosomal miR-21 with high sensitivity and selectivity, showing potential application in early diagnosis of cancer.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Sha Yu, Siyu Chen, Yuan Dang, Yuanzhen Zhou, Jun-Jie Zhu
Summary: In this study, an ultrasensitive electrochemical biosensor was developed for the detection of miR-21 using cascade amplification and DNA nanotechnology. The biosensor showed wide detection range, low detection limit, and excellent selectivity, making it a powerful platform for early detection and molecular diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Wen-Hsin Chang, Yi-Fang Lee, Yen-Wenn Liu, Itamar Willner, Wei-Ching Liao
Summary: A method for synthesizing DNA-based acrylamide hydrogel microcapsules loaded with quantum dots as readout signal is introduced, which can detect cancer-associated microRNAs. The detection sensitivity can be improved by coupling the microcapsules with strand displacement amplification.
Article
Chemistry, Analytical
Fengzhen Li, Delun Chen, Wang He, Juan Peng, Yang Cao, Jinchun Tu, Xiaohong Wang
Summary: An electrochemical biosensor based on competitive single-stranded DNA modification was established to detect Scrub typhus, with a wide linear detection range achieved by combining nano-flower NiFe layered double hydroxide (NiFe-LDH) with gold nanoparticles. The competitive relationship between beacon DNA (B-DNA) and Scrub typhus DNA (T-DNA) reduced experimental procedures and response time, enabling fast electrochemical detection of DNA, providing a promising avenue for disease diagnosis.
Article
Materials Science, Multidisciplinary
Yu Wang, Linlu Wu, Zheng Wei, Zijia Liu, Peng Cheng, Yiqi Zhang, Baojie Feng, Guangyu Zhang, Wei Ji, Kehui Wu, Lan Chen
Summary: Quantum confinement has significant effects on the band structure and optoelectronic performance of semiconducting materials. This study detected the quantized electronic states of few-layered MoS2 and investigated the influence of interlayer hybridization and substitutional defects on these states. The results provide insights into the manipulation of electronic states in few-layered TMDs and suggest potential applications in subband transitions, spin splitting, photoexcitation, and electronic devices.
Article
Chemistry, Multidisciplinary
Jianfeng Ma, Lingbo Gong, Yingying Cen, Lin Feng, Yan Su, Xingfen Liu, Jie Chao, Ying Wan, Shao Su, Lianhui Wang
Summary: Selective and sensitive detection of trace microRNA is achieved through a triple signal amplification strategy combining target-triggered cyclic strand displacement reaction (TCSDR), hybridization chain reaction (HCR), and enzyme catalytic amplification. This strategy shows higher electrochemical response, wider dynamic range, and lower detection limit for miRNA-21, demonstrating excellent selectivity, reproducibility, and stability. The proposed electrochemical biosensor successfully detects miRNA-21 in 10 HeLa cell lysates, suggesting its promising application in clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biophysics
Hanjun Chen, Ying Liu, Shaoqiong Feng, Yu Cao, Tingting Wu, Zhihong Liu
Summary: A cotton thread-based multi-channel photothermal biosensor was developed for simultaneous detection of multiple breast cancer-related miRNAs. The biosensor exhibited excellent specificity and sensitivity, with detection limits of 37 pM for miRNA-10b, 38 pM for miRNA-27a, and 38 pM for miRNA-let-7a. The biosensor also showed great potential in clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Ming-Tse Kuo, Benny Wei-Yun Hsu, Yi-Sheng Lin, Po-Chiung Fang, Hun-Ju Yu, Alexander Chen, Meng-Shan Yu, Vincent S. Tseng
Summary: Bacterial keratitis is a common vision-threatening infection, and different deep learning algorithms show promising potential in diagnosing the disease through external eye photos, with comparable diagnostic accuracy to ophthalmologists.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Stephanie L. Morais, Piedade Barros, Marlene Santos, Cristina Delerue-Matos, Andreia C. Gomes, M. Fatima Barroso
Summary: This study developed a disposable electrochemical genosensor for detecting toxic dinoflagellate, Alexandrium minutum, by immobilizing a specific DNA probe onto screen-printed gold electrodes. The genosensor showed a concentration range from 0.12 to 1.0 nM with a limit of detection of 24.78 pM and successfully analyzed the targeted genomic DNA from toxic dinoflagellates in the Atlantic Ocean.
Article
Chemistry, Analytical
Jiarui Yang, Pengfei Dong, Yikun Wang, Tianrui Liu, Yuanyuan Huang, Jianping Lei
Summary: In this study, a specific and reliable detection strategy based on stepwise recognition was developed for tandem detection of metal ions and enzymes, achieving a signal-on detection of telomerase. The electrochemical biosensor showed high sensitivity and specificity, allowing discrimination of telomerase activity among different cell lines, providing a novel method for monitoring and diagnosis of diseases.
Article
Chemistry, Medicinal
Zhen Song, Qiang-Yan Zhang, Jia-Jing Li, Jing-Lian Su, Yuan-Hua Liu, Gong-Jun Yang, Huai-Song Wang
Summary: Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was used to initiate the detection of let-7a, followed by analysis using lateral flow assay (LFA) and electrochemical impedance spectroscopy.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Riham Zayani, Amal Rabti, Sami Ben Aoun, Noureddine Raouafi
Summary: The study introduces a novel method for sensitive detection of miRNAs levels using a dual signal readout system involving competitive hybridization of miR-21 target and its biotinylated analog. The bioplatform demonstrates low detection limits, wide linear calibration range, excellent selectivity towards non-complementary miRs, and acceptable distinction with single nucleotide mismatched sequences. Furthermore, the method was successfully used to determine miR-21 expression levels in blood sera from breast cancer patients.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Susanne Hellmuth, Christopher Poehlmann, Andreas Brown, Franziska Boettger, Mathias Sprinzl, Olaf Stemmann
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Chemistry, Analytical
Christopher Poehlmann, Irina Dieser, Mathias Sprinzl
Article
Biochemical Research Methods
Martin Humenik, Christopher Poehlmann, Yiran Wang, Mathias Sprinzl
BIOCONJUGATE CHEMISTRY
(2008)
Article
Biophysics
Christopher Poehlmann, Martin Humenik, Mathias Sprinzl
BIOSENSORS & BIOELECTRONICS
(2009)
Article
Biophysics
Christopher Poehlmann, Yiran Wang, Martin Humenik, Bernd Heidenreich, Manfred Gareis, Mathias Sprinzl
BIOSENSORS & BIOELECTRONICS
(2009)
Article
Biotechnology & Applied Microbiology
Bernd Heidenreich, Christopher Poehlmann, Mathias Sprinzl, Manfred Gareis
JOURNAL OF FOOD PROTECTION
(2010)
Article
Virology
Zoe Waibler, Martina Anzaghe, Theresa Frenz, Astrid Schwantes, Christopher Poehlmann, Holger Ludwig, Marcos Palomo-Otero, Antonio Alcami, Gerd Sutter, Ulrich Kalinke
JOURNAL OF VIROLOGY
(2009)
Article
Chemistry, Multidisciplinary
Ekatherina Schulz, Christopher Poehlmann, Richard Dietrich, Erwin Maertlbauer, Thomas Elssner
FRONTIERS IN CHEMISTRY
(2019)
Article
Food Science & Technology
Katharina Schulz, Christopher Poehlmann, Richard Dietrich, Erwin Maertlbauer, Thomas Elssner
Review
Food Science & Technology
Christopher Poehlmann, Thomas Elssner
Article
Food Science & Technology
Thea Neumann, Maren Krueger, Jasmin Weisemann, Stefan Mahrhold, Daniel Stern, Martin B. Dorner, Cecile Feraudet-Tarisse, Christopher Poehlmann, Katharina Schulz, Ute Messelhaeusser, Dagmar Rimek, Frank Gessler, Thomas Elssner, Stephanie Simon, Andreas Rummel, Brigitte G. Dorner
Summary: The study successfully identified nine specific mAbs targeting CPE and showed their high affinity in detecting CPE. Through ELISAs, CPE can be detected specifically, which is useful for diagnosing diarrhea in clinical laboratories.
Proceedings Paper
Engineering, Biomedical
Christopher Poehlmann, Laurent Bellanger, Michal Drevinek, Thomas Elssner