Article
Multidisciplinary Sciences
Lauren Byrd-Leotis, Nan Jia, Yasuyuki Matsumoto, Dongli Lu, Yoshihiro Kawaoka, David A. Steinhauer, Richard D. Cummings
Summary: The MDCK cell line is a useful in vitro model for studying influenza A virus infection, and the engineered cell lines SIAT1 and hCK, which express higher levels of sialic acid, are recognized by many human IAVs. Analysis of the N-glycan structures in these cells revealed significant differences compared to the parental MDCK cell line, including different types of glycans and modifications. These differences may impact the infectivity of IAVs.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Brian C. Hauck, Charles S. Harden, Vincent M. McHugh
Summary: The search for suitable ion mobility spectrometry (IMS) calibrant compounds continues, with a focus on accurately measuring reduced ion mobility (K-0) values under various instrumental conditions. Isoflurane (IsoF) is highlighted as a strong candidate for negative ion mode calibrant, and its accurately measured K-0 values are discussed in this report. The evaluation of IsoF as a negative ion mode calibrant for IMS applications is the main focus of this study.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaomei Zhou, Woran Song, Milos V. Novotny, Stephen C. Jacobson
Summary: Structural isomers of sialylated N-glycans contribute to the diversity of the N-glycome and can be easily distinguished by mass spectrometry. An improved alkylamidation method with a delactonization step was used to enrich and stabilize sialylated N-glycans for structural characterization.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Dinko Soic, Zvonimir Mlinaric, Gordan Lauc, Olga Gornik, Mislav Novokmet, Toma Keser
Summary: The essential role of glycosylation in protein functioning has been widely recognized, leading to the development of glycan analysis techniques. Derivatization of glycans is often required for their separation and detection. In this study, the influence of fluorescent labels on glycan ionization in negative MS mode was investigated. Results showed that MS parameters and instrument configuration had a greater effect on ionization than the labels themselves.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jieqiang Zhong, Yifan Huang, Yehia Mechref
Summary: This study presents an efficient derivatization method for discriminating sialic acid linkage isomers on glycopeptides, resulting in increased signal and successful characterization of sialylated glycopeptides from various proteins. The method targets site- and sialic acid linkage-specific profiling of glycosylation, making it a facile and highly reproducible technique.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yueyue Li, Ye Peng, Haojie Lu
Summary: The translation explores the significance of protein glycosylation in diversifying protein function and highlights the importance of analyzing N-glycans and linked isomers of sialylated N-glycans. The review focuses on the role of α-2,3 and α-2,6-linked sialylated N-glycans in various physiological and pathological processes, as well as the application of mass spectrometry in biomedical research.
ACTA CHIMICA SINICA
(2021)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Edyta Budzynska, Izabela Wolanska, Jaroslaw Puton
Summary: The presented work focuses on the impact of ionization mechanisms on detection sensitivity in negative-mode ion mobility spectrometry. The research was conducted on selected organic analytes using DT IMS in the negative mode of operation. Two ionization mechanisms, electron capture and ion-molecule reactions, can be used in the negative mode of detection depending on the carrier gas. The experiments were carried out using air and nitrogen as carrier gases, allowing for a comparison of the ionization mechanisms. The study also investigated the effect of oxygen and water admixtures in carrier gases on the detection efficiency of selected organic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Hua Zhang, Xudong Shi, Yuan Liu, Bin Wang, Meng Xu, Nathan Welham, Lingjun Li
Summary: Spatial visualization of N-glycans in clinical tissue samples is achieved through a novel on-tissue derivatization strategy. This strategy utilizes the amidation reaction between sialic acid residues and aniline to stabilize and enhance the signal intensity of sialylated N-glycans. Proof-of-concept experiments and mass spectrometry imaging demonstrate the improved detection sensitivity and characterization of N-glycan dysregulation in cancer tissue samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Taylor E. Gray, Kamal Narayana, Alexander M. Garner, Samantha A. Bakker, Rachael K. H. Yoo, Amanda J. Fischer-Tlustos, Michael A. Steele, Wesley F. Zandberg
Summary: The study confirmed the existence of sulfated and sialylated BMOs and their direct biosynthetic competition, as well as identified over 170 unique BMOs, including 14 unique glucuronic acid-containing trisaccharides.
Article
Biochemical Research Methods
Salome Poyer, Ilham Seffouh, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jose L. Neira, Jean-Yves Salpin, Regis Daniel
Summary: The study developed a new analytical method for efficient differentiation and sensitive detection of different structures of chondroitin sulfate glycosaminoglycans, which was applied to analyze the disaccharide composition of biological reaction media.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Nina M. Frerichs, Sofia el Manouni el Hassani, Nancy Deianova, Mirjam M. van Weissenbruch, Anton H. van Kaam, Daniel C. Vijlbrief, Johannes B. van Goudoever, Christian V. Hulzebos, Boris. W. Kramer, Esther J. d'Haens, Veerle Cossey, Willem P. de Boode, Wouter J. de Jonge, Alfian N. Wicaksono, James A. Covington, Marc A. Benninga, Nanne K. H. de Boer, Hendrik J. Niemarkt, Tim G. J. de Meij
Summary: Early detection of late-onset sepsis (LOS) in preterm infants is essential. Fecal volatile organic compounds (VOCs) can serve as a non-invasive biomarker for detecting LOS. The study analyzed fecal samples of LOS infants and matched controls using gas chromatography-ion mobility spectrometry and gas chromatography-time of flight-mass spectrometry. Differences in VOCs were found before the onset of Escherichia coli LOS and gram-negative LOS. This study highlights the potential of VOCs as a non-invasive preclinical diagnostic biomarker for LOS.
Article
Biology
Tanya R. McKitrick, Steffen M. Bernard, Alexander J. Noll, Bernard C. Collins, Christoffer K. Goth, Alyssa M. McQuillan, Jamie Heimburg-Molinaro, Brantley R. Herrin, Ian A. Wilson, Max D. Cooper, Richard D. Cummings
Summary: The novel lamprey antibody, O6, was developed to explore the expression of 3-O-sulfated galactose on glycoproteins in humans. The study revealed that O6 can identify various human tissues, consistent with the expression of corresponding sulotransferases (GAL3ST-2 and GAL3ST-3).
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
David J. Harvey, Anna-Janina Behrens, Max Crispin, Weston B. Struwe
Summary: Negative ion collision-induced dissociation of underivatized N-glycans has been shown to be a powerful method for structural determination. This study identified a series of GalNAc-capped hybrid and complex N-glycan structures, mainly derived from recombinant glycoproteins from HIV. Analysis using TWIMS, negative ion CID, and exoglycosidase digestions revealed 26 distinct N-glycan structures and their variants. Negative ion fragmentation of glycans produces unique fingerprints that help in determining their structural identity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lauren E. Pepi, Franklin E. Leach, Dustin R. Klein, Jennifer S. Brodbelt, I. Jonathan Amster
Summary: This study investigates the potential of UVPD in distinguishing chondroitin sulfate GAG epimers and the impact of experimental parameters on this differentiation. Results suggest that UVPD has promising capabilities in differentiating GAG structures, particularly in characterizing 4-O sulfation in CS-A GAGs.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Multidisciplinary Sciences
Elisabeth E. L. Nystrom, Beatriz Martinez-Abad, Liisa Arike, George M. H. Birchenough, Eric B. Nonnecke, Patricia A. Castillo, Frida Svensson, Charles L. Bevins, Gunnar C. Hansson, Malin E. Johansson
Summary: Intestinal goblet cells are not homogeneous, with several distinct subtypes forming two differentiation trajectories. Intercrypt goblet cells located at the colonic luminal surface produce mucus with different properties from crypt-residing goblet cells. Defective icGCs in mice lead to increased sensitivity to chemically induced colitis and spontaneous colitis with age, while alterations in mucus and reduced numbers of icGCs are observed in patients with ulcerative colitis, highlighting the importance of icGCs in maintaining functional epithelial protection.
Article
Immunology
Alaullah Sheikh, Tamding Wangdi, Tim J. Vickers, Bailey Aaron, Margot Palmer, Mark J. Miller, Seonyoung Kim, Cassandra Herring, Rita Simoes, Jennifer A. Crainic, Jeffrey C. Gildersleeve, Sjoerd van der Post, Gunnar C. Hansson, James M. Fleckenstein
Summary: Enterotoxigenic Escherichia coli (ETEC) strains produce heat-labile and/or heat-stable toxins. Recent studies have found a significant association between strains that produce EatA protein and symptomatic infection. The heat-labile toxin enhances the protective barrier, while EatA degrades the mucin barrier to promote bacterial access to host cells and toxin delivery.
INFECTION AND IMMUNITY
(2022)
Article
Multidisciplinary Sciences
Jack D. A. Sharpen, Brendan Dolan, Elisabeth E. L. Nystrom, George M. H. Birchenough, Liisa Arike, Beatriz Martinez-Abad, Malin E. Johansson, Gunnar C. Hansson, Christian Recktenwald
Summary: This study demonstrates the importance of transglutaminase 3 activity in maintaining the stability of the colonic mucus layer. Lack of this enzymatic activity increases susceptibility to DSS-induced colitis in mice.
NATURE COMMUNICATIONS
(2022)
Article
Critical Care Medicine
Oanh N. Hoang, Anna Ermund, Ana M. Jaramillo, Dalia Fakih, Cory B. French, Jose R. Flores, Harry Karmouty-Quintana, Jesper M. Magnusson, Giorgio Fois, Michael Fauler, Manfred Frick, Peter Braubach, Joshua B. Hales, Richard C. Kurten, Reynold Panettieri, Leoncio Vergara, Camille Ehre, Roberto Adachi, Michael J. Tuvim, Gunnar C. Hansson, Burton F. Dickey
Summary: MUC5AC and MUC5B can be packaged within individual secretory granules in both mice and humans, either together or separately. This study provides important insights into the pathogenesis of chronic lung diseases.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ana S. Luis, Arnaud Basle, Dominic P. Byrne, Gareth S. A. Wright, James A. London, Chunsheng Jin, Niclas G. Karlsson, Gunnar C. Hansson, Patrick A. Eyers, Mirjam Czjzek, Tristan Barbeyron, Edwin A. Yates, Eric C. Martens, Alan Cartmell
Summary: This study uses structural biology to uncover how sulfatases from the human gut microbiota recognize sulfated glycans. The data reveals that carbohydrate sulfatases are highly selective for the glycan component of their substrate.
NATURE CHEMICAL BIOLOGY
(2022)
Correction
Biochemistry & Molecular Biology
Ana S. Luis, Arnaud Basle, Dominic P. Byrne, Gareth S. A. Wright, James A. London, Chunsheng Jin, Niclas G. Karlsson, Gunnar C. Hansson, Patrick A. Eyers, Mirjam Czjzek, Tristan Barbeyron, Edwin A. Yates, Eric C. Martens, Alan Cartmell
NATURE CHEMICAL BIOLOGY
(2022)
Review
Respiratory System
Camille Ehre, Gunnar C. Hansson, David J. Thornton, Lynda S. Ostedgaard
Summary: Cystic fibrosis (CF) is a genetic disorder caused by dysfunction of the CFTR protein, leading to mucus accumulation in the lungs, intestines, and pancreatic ducts. This review article discusses recent progress in understanding CFTR expression in different airway regions and cells, as well as the production and clearance of mucus from the lungs. Animal models are also explored for insights into the organization of the mucin network and the protective role of mucus and antimicrobial activities against invading pathogens.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Article
Biochemistry & Molecular Biology
Brendan Dolan, Anna Ermund, Beatriz Martinez-Abad, Malin E. Johansson, Gunnar C. Hansson
Summary: This study found that cholinergic stimulation can coordinate and rapidly eliminate bacteria from the small intestinal crypt through the activation of muscarinic acetylcholine receptors. Cholinergic stimulation induces the expansion and intracellular rupture of mucin granules, leading to the secretion of expanding mucus. Mucus secretion and expansion rely on calcium signaling, gap junctions, and ion and water transport by neighboring enterocytes.
Editorial Material
Biochemistry & Molecular Biology
Gunnar C. Hansson
Summary: This study identifies QSOX1 as a novel regulator of mucus by controlling mucin sialylation, which is important for the balance of mucus and bacteria.
Article
Cell Biology
George M. H. Birchenough, Bjoern O. Schroeder, Sinan Sharba, Liisa Arike, Christian V. Recktenwald, Fabiola Puertolas-Balint, Mahadevan V. Subramani, Karl T. Hansson, Bahtiyar Yilmaz, Sara K. Linden, Fredrik Backhed, Gunnar C. Hansson
Summary: Intestinal mucus barriers are important for preventing microbial infections but can be disrupted by a Western-style diet, leading to susceptibility to pathogen colonization.
Article
Multidisciplinary Sciences
Pablo Gallego, Maria-Jose Garcia-Bonete, Sergio Trillo-Muyo, Christian V. Recktenwald, Malin E. V. Johansson, Gunnar C. Hansson
Summary: The MUC2 mucin is crucial for building the intestinal mucus layers that separate the intestinal microbiota from the host epithelium. Researchers have generated a structural model of MUC2-C, which reveals its different domains and an additional disulfide bond at the N-terminal, essential for a stable mucus barrier.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Cynthia Buelck, Elisabeth E. L. Nystroem, Tomas Koudelka, Michael Mannbar-Frahm, Gerrit Andresen, Mariem Radhouani, Florian Tran, Franka Scharfenberg, Friederike Schrell, Fred Armbrust, Eileen Dahlke, Bei Zhao, Alex Vervaeke, Franziska Theilig, Philip Rosenstiel, Philipp Starkl, Stephan P. Rosshart, Helmut Fickenscher, Andreas Tholey, Gunnar C. Hansson, Christoph Becker-Pauly
Summary: The metalloproteases meprin alpha and meprin beta are highly expressed in the healthy gut but significantly decreased in inflammatory bowel disease. They form covalently linked heterodimers in the colon, and the major intestinal substrate for these heterodimers is galectin-3. The cleavage of galectin-3 by meprin alpha/beta heterodimers may play a key role in colon host-microbiome homeostasis.
Article
Multidisciplinary Sciences
Henriette Arnesen, Turhan Markussen, George Birchenough, Signe Birkeland, Elisabeth E. L. Nystroem, Gunnar C. Hansson, Harald Carlsen, Preben Boysen
Summary: By raising laboratory mice in a farmyard-type environment, the intestinal barrier properties can be altered, leading to a stronger protection against disease.
SCIENTIFIC REPORTS
(2023)
Article
Respiratory System
Ana M. Rodriguez-Pineiro, Florian Jaudas, Nikolai Klymiuk, Andrea Baehr, Gunnar C. Hansson, Anna Ermund
Summary: Proteins in the airway surface liquid and mucus fraction separate into distinct clusters, with increased levels of MUC5AC and MUC5B after carbachol stimulation. In newborn CF piglets, an additional cluster containing acute phase proteins suggests early inflammatory response or altered lung development. Treatment for CF airway disease should start immediately after diagnosis.
RESPIRATORY RESEARCH
(2023)
Article
Respiratory System
Anna Ermund, Lauren N. Meiss, Brendan Dolan, Florian Jaudas, Lars Ewaldsson, Andrea Bahr, Nikolai Klymiuk, Gunnar C. Hansson
Summary: The study revealed a novel form of mucus called “mucus threads” secreted from surface goblet cells, with a diameter less than two micrometers. Over time, mucus threads coalesced into larger mucus assemblies, effectively capturing particles. The complexity and variability of normal airway mucus structure and function were highlighted, emphasizing the importance of studying young subjects for understanding lung function.
RESPIRATORY RESEARCH
(2021)
