Article
Chemistry, Analytical
Irina Oganesyan, Joanna Hajduk, Julian A. Harrison, Adrien Marchand, Martin F. Czar, Renato Zenobi
Summary: This study characterizes the A2 N-glycans and their conformers using ion mobility spectroscopy, hydrogen/deuterium exchange, and electron capture dissociation. It identifies multiple gas-phase structures for each N-glycan, and successfully distinguishes and identifies each isomer using collision cross section and fragmentation analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
A. Bianco, I Neefjes, D. Alfaouri, H. Vehkamaki, T. Kurten, L. Ahonen, M. Passananti, J. Kangasluoma
Summary: Mass spectrometry is an effective method for identifying and quantifying environmentally relevant molecules, but it cannot distinguish between isomers. This study combines an experimental set-up using a differential mobility analyser with computational ion mobility calculations to successfully identify isomers. The experimental set-up allows for analysis in both the gas and liquid phase, making it a versatile tool for isomer separation.
Review
Chemistry, Analytical
Thamina Acter, Nizam Uddin, Eunji Cho, Eleanor Riches, Pierre Giustie, Carlos Afonso, Sunghwan Kim
Summary: Understanding the chemical composition and characteristics of petroleum requires molecular-level characterization and structural identification. Mass spectrometry techniques, especially ultrahigh-resolution mass spectrometry and its coupling with other complementary tools, are important analytical methods. Ion mobility coupled with high-resolution mass spectrometry has proven to be valuable in structural characterization of petroleum samples. This review provides instructive examples and future perspectives of the application of ion mobility mass spectrometry in petroleum analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao, Xiaohong Qian, Tao Zhu, Wantao Ying
Summary: Posttranslational modifications of antibody products have a significant impact on their quality attributes. This study aimed to comprehensively characterize the posttranslational modification status of antibody drug charge isomers (CIs) and investigate the effect of modifications on charge heterogeneity. Through structural characterization at different levels, the study revealed the dynamics of glycoforms and the importance of sialic acid modification in accounting for charge heterogeneity.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Ning Tang, Ya Cai, Jin-Lin Ma, Hang Ye, Zhou-Yang Xiang
Summary: Oil-tea camellia fruit shell (CFS) is a highly abundant lignocellulosic waste resource. The current composting and burning treatments of CFS pose a severe environmental threat. However, the chemical structures of the hemicelluloses in CFS have not been extensively studied, which limits their high-value utilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Analytical
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: Carbohydrates are the most abundant organic compounds in nature, and understanding their structure properties through mass spectrometry technology is crucial for their applications, especially in characterizing herbal and food polysaccharides. The development of ionization sources and mass analysers in MS has opened up new possibilities for research in this area.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Sandra M. Camunas-Alberca, Maria Moran-Garrido, Jorge Saiz, Alberto Gil-de-la-Fuente, Coral Barbas, Ana Gradillas
Summary: It is important to conduct a detailed molecular structure analysis of isomeric lipids to understand their biological roles better. Conventional tandem mass spectrometry (MS/MS) is complicated by isomeric interference, necessitating the development of specialized methodologies to separate lipid isomers. This review discusses recent lipidomic studies using ion mobility spectrometry combined with mass spectrometry (IMS-MS) and describes examples of separating and elucidating the structural and stereoisomers of lipids based on their ion mobility behavior.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Analytical
Katerina V. Djambazova, Martin Dufresne, Lukasz G. Migas, Angela R. S. Kruse, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Summary: Gangliosides are diverse acidic glycosphingolipids with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can be used for spatial analysis of gangliosides directly from tissue sections. The integration of trapped ion mobility spectrometry with MALDI IMS enables the analysis of isomeric lipid structures in situ. Gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b, with different sialic acid residue positions, were demonstrated in multiple samples, including tissue sections.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Francis Berthias, Berwyck L. J. Poad, Hayden A. Thurman, Andrew P. Bowman, Stephen J. Blanksby, Alexandre A. Shvartsburg
Summary: The prevalence and functional importance of isomeric biomolecules in biochemistry is a hot topic, but identifying and distinguishing them can be challenging due to identical mass. Modern mass spectrometry offers compositional assignments, but proteomics and lipidomics are still facing difficulties in deciphering functional networks due to lack of isomeric depth. The introduction of a new platform utilizing high-resolution differential ion mobility spectrometry (FAIMS) and ozone-induced dissociation (OzID) provides a solution for identifying isomeric lipids. Metal cationization improves FAIMS resolution, but the yield diminishes for certain metals like Cu+. Argentination strikes a balance between separation efficiency and diagnostic fragmentation for optimal FAIMS/OzID performance.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Yang Li, Yujiao Qin, Songchang Wei, Ling Ling, Chuan-Fan Ding
Summary: Steroids are important indicators of health and disease. Differentiating between steroids, especially their isomers, can be challenging due to their structural similarities. This study presents a trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) approach based on steroid analogue adduction for specific identification of isomers. The use of dexamethasone (DEX) as a heterodimer enhancer allowed successful separation of isomer pairs in TIMS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Christian Ieritano, Daniel Rickert, Joshua Featherstone, John F. Honek, J. Larry Campbell, J. C. Yves Le Blanc, Bradley B. Schneider, W. Scott Hopkins
Summary: The study demonstrates that adding solvent vapor to DMS can stabilize protonated peptides, reduce collision-induced fragmentation, and lower temperatures. Additionally, the solvation propensity of solvents aids in stabilizing charge density.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemistry & Molecular Biology
Wojciech Fabianowski, Miroslaw Maziejuk, Monika Szyposzynska, Monika Wisnik-Sawka
Summary: The article presents a technique of differential ion mobility spectrometry (DMS) for the detection and identification of volatile organic compounds (VOCs) from various categories. It analyzes the possibility of mixture identification using only the DMS spectrometer and provides several examples. The article also discusses the analysis of different compounds, their mechanisms of fragmentation, influence on effective ion temperature, and high electric field intensity.
Article
Chemistry, Analytical
Qingyu Hu, Yuting Sun, Xiyan Mu, Yulan Wang, Huiru Tang
Summary: Direct-infusion tandem mass spectrometry (DI-MS/MS) is valuable for quantitative metabolomics, MS imaging, and single cell studies, but cannot differentiate isomers/isobars with similar MS spectral features. In this study, the fragmentation pathways and characteristics of differential ion-mobility spectrometry (DMS) for four organic compounds were comprehensively investigated. A DI-DMS-MS/MS method was developed to simultaneously quantify these compounds. The method showed high accuracy and low limit-of-quantification, and clear differences between human and mouse urine samples were observed.
Article
Chemistry, Multidisciplinary
Dylan H. Ross, Joon-Yong Lee, Aivett Bilbao, Daniel J. Orton, Josie G. Eder, Meagan C. Burnet, Brooke L. Deatherage Kaiser, Jennifer E. Kyle, Xueyun Zheng
Summary: A Python tool called LipidOz was developed for the automated determination of lipid double bond positions from complex LC-OzID-IMS-MS data, using a combination of traditional automation and deep learning approaches. The development of this tool provides practical application for lipidomics research.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Engineering, Environmental
Matthias J. A. Vink, Fred A. M. G. van Geenen, Giel Berden, Timothy J. C. O'Riordan, Peter W. A. Howe, Jos Oomens, Simon J. Perry, Jonathan Martens
Summary: Agrochemicals undergo various chemical and metabolic transformations in the environment, resulting in a wide range of derivatives. In this study, the alternative method of infrared ion spectroscopy is used to identify hydroxylated derivatives of plant protection compounds in food samples.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Antony D. St-Jacques, Natalie M. Rachel, Dan R. Curry, Steve M. F. G. Gillett, Christopher M. Clouthier, Jeffrey W. Keillor, Joelle N. Pelletier, Roberto A. Chica
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2016)
Article
Chemistry, Multidisciplinary
Antony D. St-Jacques, Ian W. Wyman, Donal H. Macartney
CHEMICAL COMMUNICATIONS
(2008)
Article
Chemistry, Multidisciplinary
Gleb G. Mironov, Antony D. St-Jacques, Alexander Mungham, Matthew G. Eason, Roberto A. Chica, Maxim V. Berezovski
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Article
Multidisciplinary Sciences
Marilyn N. Vera-Chang, Antony D. St-Jacques, Remi Gagne, Chris J. Martyniuk, Carole L. Yauk, Thomas W. Moon, Vance L. Trudeau
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Antony D. St-Jacques, Joshua M. Rodriguez, Matthew G. Eason, Scott M. Foster, Safwat T. Khan, Adam M. Damry, Natalie K. Goto, Michael C. Thompson, Roberto A. Chica
Summary: This study presents a computational method to tune the conformational landscape of enzymes for enhanced catalytic efficiency with non-native substrates. By redesigning the conformational states and equilibria of an aminotransferase, the authors achieved customized biocatalysts with increased activity and altered substrate selectivity.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Kelly A. Robinson, Antony D. St-Jacques, Sam W. Shields, Amanda Sproule, Zerihun A. Demissie, David P. Overy, Michele C. Loewen
Summary: A study found that a fungus called Clonostachys rosea is able to convert 15-acetyl-deoxynivalenol (15-ADON) into 15-acetyl-deoxynivalenol-3-O-glycoside (15-ADON-3G). By knocking out UDP-GTases genes in C. rosea, it was discovered that multiple UDP-GTase enzymes are involved in this conversion process. These findings contribute to our understanding of the functions and relationships of UDP-GTases in fungi and plants.
