Article
Medicine, Research & Experimental
Tiago Oliveira, Mingfeng Zhang, Eun Ji Joo, Hisham Abdel-Azim, Chun-Wei Chen, Lu Yang, Chih-Hsing Chou, Xi Qin, Jianjun Chen, Kathirvel Alagesan, Andreia Almeida, Francis Jacob, Nicolle H. Packer, Mark von Itzstein, Nora Heisterkamp, Daniel Kolarich
Summary: This study integrated transcriptomics, proteomics and glycomics data to reveal extensive remodeling of glycocalyx in MLL-r cells, identifying potential therapeutic targets and previously unknown protein targets. The research demonstrated the importance of a systematic combined multi-omics approach in providing important diagnostic information that may be missed with a single omics technology.
Article
Biochemical Research Methods
Jiaxue Li, Lushuang Huang, Yanting Guo, Kellye A. A. Cupp-Sutton, Si Wu
Summary: Capillary electrophoresis mass spectrometry (CE-MS) is an analytical tool for microscale biological sample analysis. We developed a spray-capillary device for low-volume sample extraction and online CE-MS analysis. To optimize the performance, we developed an automated platform for high-throughput analysis using a CE autosampler.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Nicolai Bjodstrup Palstrom, Rune Matthiesen, Lars Melholt Rasmussen, Hans Christian Beck
Summary: The human plasma proteome reflects the physiological state of the cardiovascular system and has been used to analyze plasma biomarkers for cardiovascular diseases for decades. However, current plasma biomarkers only address a limited subset of cardiovascular diseases, and there is an urgent need for biomarkers for diseases with increasing incidence like heart failure and abdominal aortic aneurysm. Recent advancements in technologies for analyzing the human plasma proteome are addressing the complexity and facilitating the discovery of novel biomarkers for cardiovascular diseases.
Article
Biochemical Research Methods
Qibin Zhang, Jongmin Woo
Summary: Mass spectrometry-based clinical proteomics requires a platform that can achieve high throughput, reproducibility, robustness, and comprehensive coverage for blood plasma proteome profiling. The streamlined High-Throughput Plasma Proteomics (sHTPP) platform developed in this study meets these requirements and has demonstrated its promising potential in clinical settings. With a throughput of 60 samples per day, it can consistently identify over 600 protein groups, including FDA-approved biomarkers, from whole human plasma.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Ze-Hui Wei, Xue Zhang, Xue Zhao, Ya-Jie Jiao, Yan-Ping Huang, Zhao-Sheng Liu
Summary: This study successfully constructed a microfluidic platform for online protein separation, denaturation, digestion, and peptide enrichment, significantly improving sample processing efficiency and protein/peptide identification capacity.
Article
Surgery
Austin M. Eckhoff, Ashton A. Connor, Julie K. M. Thacker, Dan G. Blazer, Harvey G. Moore, Randall P. Scheri, Sandhya A. Lagoo-Deenadayalan, David H. Harpole, Keri A. Seymour, J. Todd Purves, Kadiyala V. Ravindra, Kevin W. Southerland, Daniel J. Rocke, Jennifer B. Gilner, Daniel C. Parker, James R. Bain, Michael J. Muehlbauer, Olga R. Ilkayeva, David L. Corcoran, Jennifer L. Modliszewski, Nicolas Devos, Matthew W. Foster, M. Arthur Moseley, Holly K. Dressman, Cliburn Chan, Janet L. Huebner, Scott Chasse, Linda Stempora, Mary E. Aschenbrenner, Mary-Beth Joshi, Beth Hollister, Ricardo Henao, Richard T. Barfield, Mark A. Ellison, Sean Bailey, Stephen Woody, Erich S. Huang, Allan Kirk, E. Shelley Hwang
Summary: The project aims to design a prospective biospecimen repository that integrates clinical data and multi-omics assays to study controlled injury and healing mechanisms. Methods involve collecting samples from patients undergoing 14 surgeries and utilizing genomic, transcriptomic, proteomic, and metabolomic assays to describe their responses to injury.
Article
Chemistry, Multidisciplinary
Himani Sharma, Vivek Yadav, Crislyn D'Souza-Schorey, David B. Go, Satyajyoti Senapati, Hsueh-Chia Chang
Summary: This study introduces a high-throughput and high-yield fractionation technique for extracellular nanocarriers based on their distinct isoelectric points. The technique utilizes a linear pH profile produced by water-splitting at a bipolar membrane and stabilized by flow. The performance of the technique is demonstrated in various biofluids, showing high purity, high yield, and probe-free isolation of nanocarriers.
Article
Chemistry, Analytical
Yassel Ramos, Alexis Almeida, Jenis Carpio, Arielis Rodriguez-Ulloa, Yasser Perera, Luis J. Gonzalez, Jacek R. Wisniewski, Vladimir Besada
Summary: In this study, a fractionator device based on continuous gel electrophoresis/electroelution sorting was created for effective separation of complex protein and peptide mixtures. The device showed higher resolution and protein recovery compared to conventional fractionation methods, leading to an improved proteome characterization.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Geriatrics & Gerontology
Daehwan Kim, Dobri D. Kiprov, Connor Luellen, Michael Lieb, Chao Liu, Etsuko Watanabe, Xiaoyue Mei, Kaitlin Cassaleto, Joel Kramer, Michael J. Conboy, Irina M. Conboy
Summary: This study extrapolates animal studies on blood heterochronicity to humans and introduces a new direct method for measuring biological age. The results indicate that human aging, like in mice, is driven by age-induced molecular excess, which can be reversed by reducing this excess. Therapeutic plasma exchange promotes a shifting of the systemic proteome towards a younger state, including restoration of pro-regenerative, anti-cancer, and apoptotic regulators, as well as a more youthful profile of myeloid/lymphoid markers in circulating cells. These findings highlight the importance of circulatory regulators in molecular rejuvenation.
Review
Pharmacology & Pharmacy
Seyyed Mojtaba Mousavi, Seyyed Alireza Hashemi, Ahmad Gholami, Masoomeh Yari Kalashgrani, Neralla Vijayakameswara Rao, Navid Omidifar, Wesley Wei-Wen Hsiao, Chin Wei Lai, Wei-Hung Chiang
Summary: Smart nanoexosomes can be potential foundation for developing antiviral drugs and vaccines, facilitating the transmission of antibodies and vaccines to enhance immune responses. They enhance viral infection and transmission by mediating communication between infected and uninfected cells, potentially playing a significant role in the treatment of viral infections.
Article
Engineering, Environmental
Zheng Fu, Lun Yu, Yongkang Gai, Feng Gao, Quanyi Liu, Li Wang, Zhiguo Liu
Summary: The construction of a fully automated Al[18F]F radiochemistry system can improve the reproducibility and reliability of Al[18F]F-based radiopharmaceuticals, and facilitate the translation from preclinical to clinical studies. This study aimed to address the limitations of commercially available automated radiosynthesizers by designing, assembling, and validating a custom-built radiosynthesizer for the production of clinical-grade Al[18F]F tracers.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Microbiology
Gloria Polanco, Nichollas E. Scott, Lon F. Lye, Stephen M. Beverley
Summary: This study leveraged an ultradeep proteomic approach to investigate two virulence-associated genes in Leishmania, revealing significant quantitative differences and phenotypic changes linked to LPG2 or FUT1. The work advanced knowledge by identifying potential proteins underlying persistence-without-pathology phenotype and mitochondrial dysfunction in mutants, shedding light on new therapeutic targets.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Reina Bassil, Kenneth Shields, Kevin Granger, Ivan Zein, Shirley Ng, Ben Chih
Summary: Researchers have developed an automated long-term culturing platform for human iPSC neurons, astrocytes, and microglia to model cellular aspects of Alzheimer's disease.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Sven Klumpe, Herman K. H. Fung, Sara K. Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S. Erdmann, Janina Baumbach, Christoph W. Mueller, Martin Beck, Juergen M. Plitzko, Julia Mahamid
Summary: Lamella micromachining by cryo-FIB at low temperature has become a widely used method for cellular cryo-electron tomography. The improved stability of new-generation cryo-FIB instruments now allows for automated operations. The SerialFIB software tool enables the creation of automated and customizable cryo-FIB preparation protocols, laying the foundation for further development of advanced cryogenic imaging and sample preparation protocols.
Article
Biochemical Research Methods
Claudia Gaither, Robert Popp, Sophia P. Borchers, Kjartan Skarphedinsson, Finnur F. Eiriksson, Margret Thorsteinsdottir, Yassene Mohammed, Christoph H. Borchers
Summary: The study demonstrated that leaving a lyophilized peptide mixture at room temperature for up to 20 days does not affect the quantitative performance of mass spectrometry analysis, making it a feasible option for sample transportation. Most peptide concentrations showed minimal fluctuations across different time points, indicating the stability of the peptide mixture under the tested conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
