期刊
ANALYTICAL CHEMISTRY
卷 82, 期 18, 页码 7649-7655出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac101413k
关键词
-
资金
- NIH NCRR [RR18522]
The ion mobility spectrometry (IMS) methods are grouped into conventional IMS, based on the absolute ion mobility, and differential or field asymmetric waveform IMS (FAIMS), based on mobility differences between strong and weak electric fields. A key attraction of FAIMS is substantial orthogonality to mass spectrometry (MS). Although several FAIMS/MS platforms were commercialized, their utility was limited by FAIMS resolving power, typically similar to 10-20. Recently, gas mixtures comprising up to 75% Ile have enabled resolving power >100 that permits separation of numerous heretofore coeluting isomers. This performance opens major new proteomic and other biological applications. Here, we show that raising the separation field by 35% over the previous 21 kV/cm provides similar or better resolution (with resolving powers of >200 for multiply charged peptides) using only 50% He, which avoids problems due to elevated pressure and He content in the mass spectrometer. The heating of ions by the separation field in this regime exceeds that at higher He content but weaker field, inducing greater izomerization of labile species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据