Review
Chemistry, Analytical
Sonam Mishra, Niraj Rajput, Tarang Jadav, Amit Kumar Sahu, Rakesh K. Tekade, Pinaki Sengupta
Summary: Accurate quantification of biomarkers has always been a challenge for many bioanalytical scientists. Liquid chromatography-tandem mass spectrometry-based methods using surrogate analytes and surrogate matrices have gained more attention due to their higher sensitivity and the ability to establish quantitative parallelism.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaomin Wang, Wouter H. J. Vaes, Esther van Duijn, Irene Nooijen, Zeen Tong, Daniel Lopes de Menezes, Stephen E. Maxwell
Summary: The study presents an AMS assay to quantify azacitidine incorporation into DNA and RNA, providing dosing optimization and target engagement assessment for AML patients with minimal blood and bone marrow samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Feng Yin, Yonghua Ling, Jason Martin, Rohini Narayanaswamy, Lisa McIntosh, Fumin Li, Guowen Liu
Summary: This study developed a LC-MS/MS assay for the quantitation of uridine and DHO in human plasma, utilizing a surrogate matrix approach with bovine serum albumin solution. The method demonstrated excellent accuracy and precision, and showed stability of uridine and DHO in human plasma under different storage conditions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Ryan A. Groves, Carly C. Y. Chan, Spencer D. Wildman, Daniel B. Gregson, Thomas Rydzak, Ian A. Lewis
Summary: Liquid chromatography mass spectrometry (LC-MS) is a widely used strategy for metabolomics analyses, offering the potential for both biomarker discovery and clinical diagnostics. However, clinical applications of LC-MS require higher throughput, which has been limited by chromatographic gradient lengths. To address this issue, researchers have developed a new analytical strategy called sequential quantification using isotope dilution (SQUID), which enables high-throughput quantification of target biomarkers in complex samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Karan Agrawal, Ramakrishna R. Voggu, Daniel Pisek, Steven Becht, Ross Chudnovskiy, Geraldine Mercier Dufour, Cecilia Arfvidsson, C. Eric Thomas
Summary: A fit-for-purpose LC-MS/MS assay for cortisol in whole blood has been developed using surrogate analytes, showing good accuracy, linearity, and stability for use in clinical trials analyzing cortisol levels. Comparability between whole blood and plasma cortisol concentrations was demonstrated, supporting the trend towards sample miniaturization and at-home sample collection.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
John T. Mehl, France Landry, Lorell Discenza, Bogdan Sleczka, Qihong Zhao, David J. Shuster, Priyanka Madia, Ruchira DasGupta, Surendran Rajendran, Huadong Sun, Eugene Ciccimaro, Timothy Olah
Summary: The study evaluated the application of generic immunoaffinity reagents in measuring surrogate mAbs, and found that anti-mouse IgG1 was effective in quantifying mouse IgG1 mAbs in mouse serum. Peptides of unknown sequence were used for multiplex LC-MS quantification.
Article
Biochemical Research Methods
Fayaj A. Mulani, Sharvani S. Nandikol, Jagadeesh S. Kajjihundi, Niranjana Pathappa, Sharanappa Puttappa, Hirekodathakallu Thulasiram
Summary: Meliaceae plants are widely used in agriculture, folklore, and traditional medicine, and contain diverse limonoids with various bioactivities. Accurate detection of these compounds from plant tissue extracts or biological samples is challenging. In this study, a new method was developed for the identification, quantification, and characterization of limonoids in different plant tissue extracts using minimal plant material.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Lucas Victor Pereira de Freitas, Ana Luisa Bigliassi Alponti, Marina Alves Damaceno, Sarah Chagas Campanharo, Agnaldo Fernando Baldo da Silva, James Jacob Sasanya, Susanne Rath, Jonas Augusto Rizzato Paschoal
Summary: This study aimed to evaluate potential procedures for levamisole incorporation in extruded fish feed using different coating agents. The results showed that the ethyl cellulose coating method had the lowest leaching rate, indicating its potential as a promising procedure for levamisole incorporation in fish feed to reduce environmental contamination.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Biochemical Research Methods
Rongmei Huang, Yi Hong, Yike Wu, Weifeng Li, Wenlan Liu
Summary: We developed a new LC-MS/MS method for simultaneous determination of serum testosterone and free testosterone levels. The method is simple to perform and can be applied in clinical practice, providing important information for diagnosis, treatment, and monitoring of androgen-related diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Kevin McKinski, Dean McNulty, Francesca Zappacosta, Mary Birchler, Matt Szapacs, Christopher Evans
Summary: ICOS, expressed on the surface of activated T-cells, is being investigated as a potential therapeutic target. Recent studies found aberrant increases in sICOS in disease-state patients using commercial ELISA kits, but the results were not consistent with in-house immunoassays. The researchers developed a novel LC-MS/MS method to confirm the results and demonstrated that sICOS is a low abundance soluble protein.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Kacey D. Cliburn, Marilyn A. Huestis, Jarrad R. Wagner, Philip M. Kemp
Summary: A liquid chromatography-tandem mass spectrometry method was developed and validated for quantifying cannabinoids in postmortem specimens, showing accuracy and stability in measuring various cannabinoids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Plant Sciences
Ana-Marija Gotal Skoko, Bojan Sarkanj, Marta Lores, Maria Celeiro, Martina Skendrovic Babojelic, Dragutin Kamenjak, Ivana Flanjak, Antun Jozinovic, Tihomir Kovac, Ante Loncaric
Summary: Apples and apple-based products are a valuable source of polyphenols in our daily diet. Recent studies have shown that traditional apple varieties contain a higher concentration of polyphenols and antioxidant activity compared to commercial varieties, highlighting their potential as a source of natural antioxidants and polyphenolics.
Article
Biochemical Research Methods
Yuanyuan Lu, Zhitong Mai, Hongxia Zhou, Wenda Guan, Shiguan Wu, Heyan Zou, Maoting Shen, Yangqing Zhan, Feng Ye, Minshan Qiu, Lihan Shen, Beibei Zhao, Zifeng Yang
Summary: This study developed a reliable and sensitive method for quantifying eicosanoids in human serum. The method showed good linearity, high recovery rates, and low limits of quantification. In clinical application, different characteristic metabolite profiles were found in patients with severe pneumonia.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Mathias Langner, Ivona Mateska, Nicole Bechmann, Ben Wielockx, Triantafyllos Chavakis, Vasileia Ismini Alexaki, Mirko Peitzsch
Summary: In this study, a simple LC-MS/MS method is described for accurately quantifying arginine and its related metabolites in different experimental samples. The method is suitable for various applications and shows good performance in quantifying arginine metabolites in cell lysates, cell culture supernatants, and tissue extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Plant Sciences
Javad Najafi, Tore Brembu, Ane Kjersti Vie, Rannveig Viste, Per Winge, Imre E. Somssich, Atle M. Bones
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
Amit Kumar Sharma, Alice Muhlroth, Juliette Jouhet, Eric Marechal, Leila Alipanah, Ralph Kissen, Tore Brembu, Atle M. Bones, Per Winge
Article
Biochemistry & Molecular Biology
Mahsa Jalili, Marco Gerdol, Samuele Greco, Alberto Pallavicini, Francesco Buonocore, Giuseppe Scapigliati, Simona Picchietti, Maria Angeles Esteban, Morten Rye, Atle Bones
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Yang Jin, Rolf Erik Olsen, Thomas Nelson Harvey, Mari-Ann Ostensen, Keshuai Li, Nina Santi, Olav Vadstein, Atle Magnar Bones, Jon Olav Vik, Simen Rod Sandve, Yngvar Olsen
Article
Plant Sciences
Hieng-Ming Ting, Boon Huat Cheah, Yu-Cheng Chen, Pei-Min Yeh, Chiu-Ping Cheng, Freddy Kuok San Yeo, Ane Kjersti Vie, Jens Rohloff, Per Winge, Atle M. Bones, Ralph Kissen
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Marianne Nymark, Marthe Caroline Gronbech Hafskjold, Charlotte Volpe, Davi de Miranda Fonseca, Animesh Sharma, Eirini Tsirvouli, Manuel Serif, Per Winge, Giovanni Finazzi, Atle Magnar Bones
Summary: Loss of CpSRP54 in the diatom affects the accumulation of chloroplast-encoded subunits and photophysiological responses, but the phenotype is relatively mild due to the activation of mechanisms alleviating the loss of CpSRP54. This suggests that plants, green algae, and diatoms have evolved differences in the pathways for inserting proteins into thylakoid membranes during translation and post-translation.
Article
Cell Biology
Ishita Ahuja, Ralph Kissen, Linh Hoang, Bjornar Sporsheim, Kari K. Halle, Silje Aase Wolff, Samina Jam Nazeer Ahmad, Jam Nazeer Ahmad, Atle M. Bones
Summary: The glucosinolate-myrosinase system plays a crucial role in Arabidopsis guard cells affecting the stomata and ultrastructure of guard cells. Mutants lacking myrosinase exhibit distinct features in guard cells and stomata complexes, suggesting a novel link between the defence system and physical barriers in plants.
Article
Biotechnology & Applied Microbiology
Amit K. Sharma, Marianne Nymark, Snorre Flo, Torfinn Sparstad, Atle M. Bones, Per Winge
Summary: The CRISPR/Cas9 system allows for simultaneous editing of multiple homologous genes by inducing indels, with potential applications in various organisms for genetic research. By utilizing a variant of Cas9 with increased specificity, off-target editing events can be significantly reduced.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Hanne-Line Rabben, Yosuke Kodama, Masahiko Nakamura, Atle Magnar Bones, Timothy Cragin Wang, Duan Chen, Chun-Mei Zhao, Anders Overby
Summary: Natural isothiocyanates from edible vegetables have shown potential as chemopreventive agents against various types of cancer. This study focused on studying the potential of isothiocyanates in chemoprevention and enhancing the efficacy of cytotoxic drugs in gastric cancer treatment. The results demonstrated that PEITC exhibited anti-cancer effects, particularly when administered before tumor initiation, suggesting a chemopreventive role in gastric cancer, and that pretreatment with PEITC enhanced the anti-cancer effects of cisplatin possibly by reducing glutathione levels, indicating a potential combination strategy for chemotherapy.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Environmental Sciences
Ana Rotter, Michele Barbier, Francesco Bertoni, Atle M. Bones, M. Leonor Cancela, Jens Carlsson, Maria F. Carvalho, Marta Ceglowska, Jeronimo Chirivella-Martorell, Meltem Conk Dalay, Mercedes Cueto, Thanos Dailianis, Irem Deniz, Ana R. Diaz-Marrero, Dragana Drakulovic, Arita Dubnika, Christine Edwards, Hjoerleifur Einarsson, Aysegul Erdogan, Orhan Tufan Eroldogan, David Ezra, Stefano Fazi, Richard J. FitzGerald, Laura M. Gargan, Susana P. Gaudencio, Marija Gligora Udovic, Nadica Ivosevic DeNardis, Rosa Jonsdottir, Marija Katarzyte, Katja Klun, Jonne Kotta, Leila Ktari, Zrinka Ljubesic, Lada Lukic Bilela, Manolis Mandalakis, Alexia Massa-Gallucci, Inga Matijosyte, Hanna Mazur-Marzec, Mohamed Mehiri, Soren Laurentius Nielsen, Lucie Novoveska, Donata Overlinge, Giuseppe Perale, Praveen Ramasamy, Celine Rebours, Thorsten Reinsch, Fernando Reyes, Baruch Rinkevich, Johan Robbens, Eric Roettinger, Vita Rudovica, Jerica Sabotid, Ivo Safarik, Siret Talve, Deniz Tasdemir, Xenia Theodotou Schneider, Olivier P. Thomas, Anna Torunska-Sitarz, Giovanna Cristina Varese, Marlen Vasquez
Summary: Coastal countries have traditionally relied on marine resources for various needs, while modern societies have increased demands for diverse products leading to the emergence of blue biotechnology. This field offers opportunities for commercial interests in pharmaceuticals, cosmetics, food, and related industries. Exploration of marine resources and their potential applications in various sectors are highlighted, along with the importance of legislation, funding, communication, and stakeholder engagement in the development of marine biotechnology.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Environmental Sciences
Prashanna Guragain, Max Tkachov, Anna Solvang Batnes, Yngvar Olsen, Per Winge, Atle M. Bones
Summary: The arthropod salmon louse poses a significant threat to Atlantic salmon aquaculture and wild salmonids, requiring effective management strategies to control infestations. Current methods, such as pesticide use and biological control, have shown partial success but none completely fulfill the necessary pest control strategy. The arms race of lice/pest management is complicated by the marine environment, making it challenging to precisely target pests and avoid unintended negative effects on general wildlife.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Ecology
Prashanna Guragain, Anna Solvang Batnes, John Zobolas, Yngvar Olsen, Atle M. Bones, Per Winge
Summary: The aquaculture industry has been combating salmon lice issues in salmonid farming by optimizing treatment effectiveness and studying genetic differences between subpopulations. Through IIb-RAD sequencing and a random forest classification algorithm, important markers for sex differentiation and regional genetic structure of Norwegian salmon lice were identified.
ECOLOGY AND EVOLUTION
(2022)
Article
Plant Sciences
Farid Shekari, Fariborz Shekari, Javad Najafi, Amin Abassi, Zahra Radmanesh, Atle Magnar Bones
Summary: This study found that extracts from Nepeta meyeri can inhibit the germination and seedling growth of Cuscuta campestris. The flower and leaf extracts showed the strongest inhibitory effects and may be recommended as potent bio-control agents for controlling Dodder.
Article
Agriculture, Multidisciplinary
S. J. N. Ahmad, W. Ahmad, D. Majeed, M. Sufian, M. Z.Sharif, A. Ali, I. Ahuja, A. M. Bones, J. N. Ahmad
Summary: The Acacia nilotica trees in Pakistan have important economic and medicinal value, while Acacia nilotica subsp. indica in Australia is a serious weed causing potential threat to forests. Efforts are being made to control the wild plant species, including using leaf-feeding insects as biological agents.
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
(2021)
Article
Zoology
Jam Nazeer Ahmad, Rashid Mushtaq, Samina Jam Nazeer Ahmad, Mubasher Ahmad Malik, Mujahid Manzoor, Muhammad Tahir, Zubair Aslam, Sumaira Maqsood, Ishita Ahuja, Atle M. Bones
PAKISTAN JOURNAL OF ZOOLOGY
(2020)