Real-Time in Vivo Simultaneous Measurements of Nitric Oxide and Oxygen Using an Amperometric Dual Microsensor

This paper reports a real-time study of the codynamical changes in the release of endogenous nitric oxide (NO) and oxygen (O-2) consumption in a rat neocortex in vivo upon electrical stimulation using an amperometric NO/O-2 dual microsensor. Electrical stimulation induced transient cerebral hypoxia due to the increased metabolic demands that were not met by the blood volume inside the stimulated cortical region. A NO/O-2 dual microsensor was successfully used to monitor the pair of real-time dynamic changes in the tissue NO and O-2 contents. At the onset of electrical stimulation, there was an immediate decrease in the cortical tissue O-2 followed by a subsequent increase in the cortical tissue NO content. The averages of the maximum normalized concentration changes induced by the stimulation were a 0.41 (+/- 0.04)-fold decrease in the O-2 and a 3.6 (+/- 0.9)-fold increase in the NO concentrations when compared with the corresponding normalized basal levels. The peak increase in NO was always preceded by the peak decrease in O-2 in all animals (11 = 11). The delay between the maximum decrease in 02 and the maximum increase in NO varied from 3.1 to 54.8 s. This rather wide variation in the temporal associations was presumably attributed to the sparse distribution of NOS-containing neurons and the individual animal's differences in brain vasculatures, which suggests that a sensor with fine spatial resolution is needed to measure the location-specific real-time NO and O-2 contents. In summary, the developed NO/O-2 dual microsensor is effective for measuring the NO and O-2 contents in vivo. This study provides direct support for the dynamic role of NO in regulating the cerebral hemodynamics, particularly related to the tissue oxygenation.