Article
Environmental Sciences
Hyeon-Jeong Lee, Minsik Kang, Junghyun Son, Hophil Min, Sangwon Cha, Han Bin Oh, Won Keun Oh, Jaeick Lee
Summary: Phenols are important environmental endocrine disruptors with potential adverse health effects. A comprehensive and sensitive analytical method for multiple classes of phenols was developed by investigating the influence of mobile phase compositions in LC-ESI-MS/MS analysis. This method allowed for the concurrent ionization of all phenols and significantly improved the analytical sensitivity for certain phenols.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Davide Coniglio, Mariachiara Bianco, Giovanni Ventura, Cosima D. Calvano, Ilario Losito, Tommaso R. Cataldi
Summary: The lipidome of the brown seaweed wakame was extensively examined, revealing a variety of phospholipids and glycolipids. Saturated fatty acid C16:0 was the most abundant, while monounsaturated C18:1 and polyunsaturated C18:2 and C20:4 chains were predominant. The study's findings may open up possibilities for lipidomic investigations on other edible marine algae, highlighting their potential as a source of bioactive lipids.
Article
Agriculture, Multidisciplinary
Sandra Muehlwald, Nina Meyburg, Sascha Rohn, Nadja Buchner
Summary: This study compared the clean-up effect of a fully automated 2D-LC system with a traditional QuEChERS LC method in negative ESI mode, focusing on the detection of compounds. The results showed that fewer compounds could be detected with 2D-LC and negative ESI mode, but for specific polar compounds, 2D-LC yielded better results.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Chemistry, Analytical
Yuanyuan Wu, Wenmei Zhang, Yaoyao Zhao, Xiayan Wang, Guangsheng Guo
Summary: Research on single-cell proteomics is crucial for understanding the growth, development, and diseases in organisms. However, the complexity and limited quantity of proteins in single cells have hindered the progress in this field. Mass spectrometry-based single-cell proteomics, particularly electrospray ionization mass spectrometry (ESI-MS), has rapidly advanced due to its minimal sample requirements, high sensitivity, and structural information without antibody labeling. Nevertheless, the variations in pretreatment methods, separation techniques, and MS instruments greatly affect the throughput and coverage of single-cell proteomic technologies. This review categorizes recent publications based on different methods for single-cell protein sample separation, highlights the performance of these methods in terms of detection coverage and throughput, and discusses the applications and future prospects of ESI-MS-based single-cell proteomics.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Zheng Wang, Aimin Li, Yufeng Liao, Chendong Shuang, Yang Pan, Yangyang Zhang, Hongfang Sun, Qing Zhou, Wentao Li
Summary: The interference of natural organic matter (NOM) on the determination of pharmaceuticals using liquid chromatography-mass spectrometry was investigated. The results showed that NOM concentrations had little effect on the recoveries of most pharmaceuticals, but had a linear negative correlation with matrix effects and process efficiencies, which decreased with increasing NOM concentrations. This study suggests that hydrophilic pharmaceuticals are more susceptible to NOM interference in the analytical process.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Nina Davtian, Nuria Penalva, Antoni Rosell-Mele, Joan Villanueva
Summary: This article describes a solid phase extraction method that utilizes the affinity between monosaccharide anhydrides (MAs) and immobilized Na+ ions for selective extraction of these non-ionic compounds. The method effectively extracts MAs from various sediment samples, producing extracts with low ion suppression effects and allowing for quantification with LC-ESI-MS/MS systems. The combination of LE-SPE and LC-ESI-MS/MS has the potential to be a sensitive and reliable technology for analyzing saccharides and amino acids in environmental and biological samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Andrea Castellaneta, Ilario Losito, Davide Coniglio, Beniamino Leoni, Pietro Santamaria, Maria Antonietta Di Noia, Luigi Palmieri, Cosima Damiana Calvano, Tommaso R. Cataldi
Summary: An automated workflow named LIPIC is proposed for correcting isotopic interference in the analysis of phospholipid mixtures by liquid chromatography, improving accuracy through precise signal intensities prediction and correction.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Herbert J. Dias, Eduardo J. Crevelin, Vinicius Palaretti, Ricardo Vessecchi, Antonio E. M. Crotti
Summary: Through negative ion ESI accurate-mass tandem and sequential mass spectrometry, it was found that deprotonated DBNs produced more diagnostic product ions than protonated molecules, providing more information and avoiding the use of additives.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Multidisciplinary Sciences
Yoon Keun Cho, Young Cheol Yoon, Hyeonyeong Im, Yeonho Son, Minsu Kim, Abhirup Saha, Cheoljun Choi, Jaewon Lee, Sumin Lee, Jae Hyun Kim, Yun Pyo Kang, Young-Suk Jung, Hong Koo Ha, Je Kyung Seong, James G. Granneman, Sung Won Kwon, Yun-Hee Lee
Summary: The dysregulation of plasmalogen metabolism in adipose tissue is associated with metabolic diseases. The deletion of adipocyte TMEM86A is protective against high fat diet induced metabolic disease, and this effect can be replicated by plasmenyl lysophosphatidylethanolamine 18:0 supplementation.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Huaxu Yu, Tao Huan
Summary: It is well known that nonlinear electrospray ionization (ESI) distorts signal distribution in mass spectrometry (MS) analysis, resulting in decreased statistical power for t-test. However, the exact consequences and potential solutions to this quantitative issue have not been thoroughly explored. In this study, using a serial diluted urine metabolomics dataset, it was demonstrated that over 80% of metabolic features exhibit nonlinear ESI response patterns, causing skewed MS signal distributions. Among them, right-skewed MS distributions cannot be corrected by conventional data transformations. Furthermore, a Monte Carlo simulation was conducted to quantitatively evaluate the reduced statistical power for t-test calculated using MS signal data under different sample sizes and effect sizes. The results consistently showed that t-test using MS signal data has lower statistical power than t-test using metabolic concentration data. To address this statistical issue, a bioinformatic workflow called PowerU was proposed, which aims to mitigate the diminished statistical power caused by both the nonlinear ESI response and the inherent non-normal distribution of metabolic concentrations. The PowerU workflow consists of two steps: converting MS signals to quality control (QC) sample injection amounts to address the skewed MS signal distributions, and performing a Shapiro-Wilk test to assess data normality and guide the application of t-test and Mann-Whitney U test for optimal statistical outcomes. PowerU was validated using a metabolomics study of mouse cecum samples, and the results showed that PowerU significantly improves the statistical power for t-test and facilitates the discovery of significantly altered metabolites for downstream biological interpretation.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Shusuke Takemine, Mamoru Motegi, Manabu Takayanagi, Sunao Usui, Ikuma Kuroda
Summary: In this study, a determination method for hydrazine in the air was developed. The method involved derivatizing hydrazine with p-dimethyl amino benzaldehyde (DBA) and analyzing the derivative using LC/MS/MS. The method showed good sensitivity, with instrument detection and quantification limits of 0.003 and 0.008 ng/mL, respectively. A silica cartridge impregnated with DBA and 1,2-bis(4-pyridyl) ethylene was used to collect hydrazine in the air, resulting in mean recovery rates of 97.6% and 92.4% for outdoor and indoor locations, respectively. The method had detection and quantification limits of 0.1 and 0.4 ng/m3, respectively, and allowed for high-throughput analyses without pretreatment or concentration steps.
Article
Chemistry, Analytical
Emmanuel Letsyo
Summary: This study establishes a simple and rapid method to determine pyrrolizidine alkaloids in honey using chromatography and mass spectrometry, in order to identify the botanical sources responsible for contamination. Identification of these plants can guide beekeepers to minimize potential liver damaging effects.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Vu Quang Lam, La Hoang Anh, Nguyen Van Quan, Tran Dang Xuan, Ichiro Hanamura, Kaori Uchino, Sivasundaram Karnan, Akiyoshi Takami
Summary: This study found that the ethyl acetate extract from Callerya speciosa root has strong antioxidant and cytotoxic activities, inhibiting the viability of multiple myeloma and mantle cell lymphoma cell lines and inducing cell apoptosis. The main compounds identified in the extract were isoflavones. These findings suggest that Callerya speciosa may be a promising natural source of antileukemia and antimyeloma agents.
Article
Chemistry, Analytical
Barbara Socas-Rodriguez, Veronika Pilarova, Margareta Sandahl, Cecilia Holm, Charlotta Turner
Summary: A new analytical method has been developed to simultaneously determine vitamin D-2 and vitamin D-3 and their hydroxylated and esterified forms. The method utilizes ultrahigh-performance supercritical fluid chromatography coupled with triple quadrupole tandem mass spectrometry for efficient separation. The method has been validated and applied for the analysis of vitamin D analogues in human plasma samples, demonstrating its applicability in biological sample analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Eirini-Maria Paschalidou, Rebecka Lindblad, Leon Zendejas Medina, Dennis Karlsson, Ulf Jansson, Leif Nyholm
Summary: The corrosion resistances of CoCrFeMnNi magnetron-sputtered thin films with different carbon concentrations were investigated in acidic environments. The results showed that the electrochemical behavior of the films, particularly in the chromium transpassive region, was significantly influenced by the carbon concentration and experimental conditions. At high potentials, the carbon-containing films exhibited better corrosion resistance in hydrochloric acid than in sulfuric acid, attributed to a slower carbon oxidation rate in hydrochloric acid, facilitating the formation of a manganese-rich oxide layer.
ELECTROCHIMICA ACTA
(2022)
Review
Chemistry, Multidisciplinary
David Rehnlund, Zhaohui Wang, Leif Nyholm
Summary: This review focuses on diffusion-controlled capacity losses in rechargeable lithium-based batteries due to concentration gradients, which have been largely unrecognized. Evidence for diffusion-controlled capacity losses is presented and discussed, along with approaches to identify and solve these problems. Future research directions in this important area are proposed.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Physical
Shizhao Xiong, Leif Nyholm, Aleksandar Matic, Chao Zhang
Summary: This article discusses the electrodeposition mechanism of lithium (Li) metal anode and its importance for rechargeable Li-metal batteries. The electrodeposition of Li is controlled by thermodynamic and kinetic factors, and the inconsistent scale of the process poses a significant challenge for experiments and simulations. The article introduces different approaches to address this challenge and explores the possible synergies between them.
MATERIALS TODAY ENERGY
(2022)
Article
Materials Science, Multidisciplinary
Maciej Kaplan, Aishwarya Srinath, Lars Riekehr, Leif Nyholm, Bjorgvin Hjorvarsson, Stefan Fritze
Summary: Amorphous TaNiSiC and TaNiC films were prepared using combinatorial magnetron sputtering, and their properties were analyzed. The results showed that the addition of Si reduced the elemental segregation in TaNiSiC, resulting in improved hardness, corrosion resistance, and thermal stability of the films.
MATERIALS & DESIGN
(2022)
Article
Chemistry, Multidisciplinary
Fiona M. Nermark, Mmilili M. Mapolelo, James Darkwa, Ola F. Wendt, Charlotta Turner
Summary: The optimal conditions for extracting sulfur from sub-bituminous coal involve using ethanol/water (90/10, v/v %) at 129 degrees C (105 bars) for 10 minutes. However, this method is not efficient in removing ash.
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: The increased interest in using lignin as a feedstock to produce aromatic compounds requires advanced chemical analysis methods for lignin characterization. This study presents a novel method for quantifying lignin dimers using supercritical fluid chromatography with charged aerosol detection. The method was validated using synthesized lignin dimers and found to be effective for quantification. The study also emphasizes the importance of chromatographic peak resolution and introduces a digital peak sharpening technique to address it.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yu-Kai Huang, Heyin Chen, Leif Nyholm
Summary: The nucleation of lithium directly on the copper current collector in anode-free lithium-metal batteries is limited by poor control and diffusion of lithium into the copper substrate via grain boundaries. Prelithiation of the copper surface improves the nucleation process by saturating the surface with lithium, reducing the influence of lithium diffusion. This leads to more homogeneous lithium nucleation, especially when a short potentiostatic nucleation pulse is applied.
Article
Chemistry, Multidisciplinary
Federica Nardella, Jens Prothmann, Margareta Sandahl, Peter Spegel, Erika Ribechini, Charlotta Turner
Summary: In this study, supercritical fluid extraction (SFE) and CO2-expanded liquid extraction (CXLE) were used to extract lignin from softwood and hardwood chips. Ethanol, acetone, and ethyl lactate were investigated as green organic co-solvents in the extractions. The optimized method using liquid CO2/acetone/water (10/72/18, v/v/v), at 60°C, 350 bar, 30 min, and 2 mL/min flow rate, successfully detected 13 lignin monomers and 6 lignin dimers from the hardwood chips. These results demonstrate the potential of supercritical fluids and green solvents in the mild and benign extraction of lignin from wood.
Article
Chemistry, Analytical
Daniel Papp, Thanya Rukkijakan, Daria Lebedeva, Tommy Nylander, Margareta Sandahl, Joseph S. M. Samec, Charlotta Turner
Summary: A novel method for universal quantification of lignin dimers based on supercritical fluid chromatography with charged aerosol detection (CAD) was developed and applied to quantify lignin dimers in an oil obtained by reductive catalytic fractionation (RCF) of birch sawdust. The applicability of linear regression and quadratic calibration curves for quantification was evaluated and found to be equally appropriate. The response factors of lignin dimers were compared and showed little variation, enabling the use of a single calibrant for these compounds. The importance of chromatographic peak resolution in CAD was stressed and addressed using a digital peak sharpening technique.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Le Anh Ma, Alexander Buckel, Andreas Hofmann, Leif Nyholm, Reza Younesi
Summary: Knowledge about capacity losses related to the solid electrolyte interphase (SEI) in sodium-ion batteries (SIBs) is still limited. This study investigates capacity losses caused by changes in the SEI layer under different electrolyte conditions, and finds that the amount of capacity lost depends on the interplay between the electrolyte chemistry and the thickness and stability of the SEI layer.
Article
Materials Science, Multidisciplinary
Leon Zendejas Medina, Marcus Vinicius Tavares de Costa, Olivier Donzel-Gargand, Leif Nyholm, E. Kristofer Gamstedt, Ulf Jansson
Summary: In this paper, the formation of metal/carbon nanocomposites based on complex high entropy alloys (HEAs) was studied. The effect of the average carbon affinity on phase formation was investigated. The results showed the presence of free carbon in the coatings at certain carbon concentrations. However, the rapid quenching during deposition resulted in amorphous coatings consisting of either a single metallic phase or a mixture of metallic phase and sp2- and sp3-hybridized carbon.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Lars Olow Simon Colbin, Charles Aram Hall, Ahmed S. Etman, Alexander Buckel, Leif Nyholm, Reza Younesi
Summary: This article investigates the impact of different electrolyte solutions on the anodic dissolution or passivation of aluminum in sodium-ion batteries. It is found that the type of electrolyte is critical for the presence of anodic dissolution, while the solvent alters the dissolution process.
Article
Engineering, Chemical
Leif Nyholm, Tove Ericson, Ahmed S. Etman
Summary: Anodic dissolution of the aluminum positive electrode current collector limits performance in high-voltage Li-ion batteries. It is most likely caused by etching of the Al2O3 passive layer by protons released during the oxidation of the solvent.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Green & Sustainable Science & Technology
Yu-Kai Huang, Jean Pettersson, Leif Nyholm
Summary: Although graphite-based composite electrodes are commonly used in lithium-ion batteries for their good cycle performance, their long-time cycling stability still needs improvement. The diffusion-controlled lithium-trapping effect, which accounts for around 30% of the total capacity loss during long-time cycling, is shown to be an additional and largely unrecognized aging mechanism in graphite-based electrodes. It is caused by incomplete delithiation due to diffusion-controlled redistribution of intercalated lithium in graphite. However, constant-voltage delithiation steps can increase the delithiation efficiency and decrease the influence of the lithium-trapping effect.
ADVANCED ENERGY AND SUSTAINABILITY RESEARCH
(2022)