Article
Chemistry, Analytical
Qing Tang, Jun Xu, Siqi Wei, Haoyi Chen, Jiapeng Chen, Huilin Zhang, Lihong Liu
Summary: An sensitive and label-free CRP assay was developed using cyclic enzymatic signal amplification and capillary electrophoresis with UV detection, resulting in a wide linear range in 1% human serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Z. Hadi, Kh. Ghanbari
Summary: In this study, a highly sensitive electrochemical sensor was developed by modifying a carbon paste electrode with an electrochemically reduced para-nitrobenzoic acid/graphene oxide nanocomposite for the measurement of uric acid. The modified electrode showed excellent performance for uric acid determination and was suitable for analysis of real samples.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Food Science & Technology
Juan David Figueroa, Noreima Barroso-Torres, Marcela Morales, Barbara Herrera, Mario Aranda, Eva Dorta, Camilo Lopez-Alarcon
Summary: The ORAC assay does not differentiate between peptides based on their chemical structures and the contribution of oxidation products. Peptides showed similar ORAC values, but the bond dissociation energy (BDE) varied.
Article
Biochemistry & Molecular Biology
Xue-Qing Liu, Xiao-Hui Yan, Jun Liang, Hai-Xue Kuang, Yong -Gang Xia
Summary: A sample preparation method based on microwave assisted free radical degradation (MFRD) was proposed to establish structural-fingerprinting of polysaccharides for quality assessment. The MFRD condition was optimized and the structures of the degradation products were analyzed. A low-polymerization compositional fingerprinting was successfully constructed using high-performance anion-exchange chromatography. Compared to conventional methods, MFRD significantly improves sample preparations and is applicable to various polysaccharide samples.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Multidisciplinary
Jiaqi Wang, Zhemiao Xie, Jiayu Alexander Liu, Guanxuan Lu, Rui Zhou, John T. W. Yeow
Summary: The development of mid-infrared (MIR) detectors is a hot research topic with significant progress in low-dimensional materials and clean-room fabrication strategies. These detectors have various applications such as industrial non-destructive testing, wearable safety monitoring, and Internet of Things. This study focuses on the photothermoelectric (PTE) mechanism in MIR detectors and proposes self-assembly carbon nanotube (CNT)-based detectors for sensitive and rapid IR detection. The results demonstrate the potential of PTE mechanism in broadening horizons for high-performance IR detectors in industrial applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Biochemistry & Molecular Biology
Gabriel Robert, J. Richard Wagner, Jean Cadet
Summary: Molecular oxygen sensitizes DNA to damage induced by ionizing radiation and other free radical-mediated reactions. It converts carbon-centered radicals within DNA into peroxyl radicals, leading to the formation of oxidized products and strand breaks. Reactive peroxyl radicals can also propagate damage to neighboring components in DNA strands, causing tandem or double lesions. This review highlights the formation and biological implications of oxidatively generated DNA tandem lesions.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Analytical
Youli Tian, Yiren Cao, Genhan Zha, Ke-Er Chen, Muhammad Idrees Khan, Jicun Ren, Weiwen Liu, Yuxing Wang, Qiang Zhang, Chengxi Cao
Summary: This study proposes a marker-free isoelectric focusing method that uses a convolutional neural network model to efficiently and accurately classify isoelectric focusing patterns. A dataset of 1449 isoelectric focusing patterns was collected and experimental pH gradient drift was introduced to enhance the model's robustness. With simple preprocessing, rapid inference speed (only 50 ms), and a high accuracy of 97.1%, the CNN model outperformed traditional algorithms in simultaneously identifying meat species and cuts, indicating its great potential for large-scale isoelectric focusing analyses of complex protein samples when combined with microstrip isoelectric focusing.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xiaomin Zhang, Ge Yang, Wenjing Liu, Qing Liu, Zhuoran Wang, Kelong Fan, Feng Qu, Yuanyu Huang
Summary: Using CE-SELEX, four candidate aptamers with a stem-loop structure were selected targeting LRP6 protein. Among them, AptLRP6-A3 showed the highest affinity with LRP6-overexpressed human breast cancer cells. This LRP6 aptamer holds promise for rapid diagnosis and treatment of LRP6-related diseases.
Article
Nanoscience & Nanotechnology
Jake Dudley Mehew, Marina Y. Timmermans, David Saleta Reig, Stefanie Sergeant, Marianna Sledzinska, Emigdio Chavez-Angel, Emily Gallagher, Clivia M. Sotomayor Torres, Cedric Huyghebaert, Klaas-Jan Tielrooij
Summary: Nanomaterials, particularly carbon nanotubes (CNTs), are promising candidates for applications requiring high thermal conductivity. In this study, the thermal conductivity of free-standing double-walled CNT films was measured using all-optical Raman thermometry, showing significantly enhanced thermal conduction compared to single-walled CNT films. This research contributes to a better understanding of these nanomaterials and their suitability for extreme ultraviolet (EUV) lithography.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Isabel Lizcano Sanz, Francisco Javier Guzman Bernardo, Gregorio Castaneda Penalvo, Juana Rodriguez Flores
Summary: In this study, DSPE was used in combination with capillary electrophoresis for the determination of dabrafenib and trametinib in serum, showing good enrichment efficiency and low detection limits under optimized conditions. The method offers a simpler and less sophisticated alternative for drug monitoring in patients with metastatic melanoma.
MICROCHEMICAL JOURNAL
(2021)
Review
Biochemical Research Methods
Zhao Meng, Junmin Bi, Qianqian Zhang, Hang Ren, Weidong Qin
Summary: The review discusses the applications of nanomaterials in capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) to enhance sensitivity of various detection techniques. It provides an overview of the types of nanomaterials used, detection mechanisms, analytical performance, and applications.
Article
Biochemical Research Methods
P. Layton Ashmore, Florentino Valdez, James F. Harbertson, Roger B. Boulton, Thomas S. Collins
Summary: A novel method using CE-UV/vis was developed for the determination of free SO2 in wine and cider. The CE method showed significant differences with conventional methods, especially in the presence of anthocyanins. The CE method was found to be rapid, sensitive, robust, and repeatable, overcoming the issue of over-reporting free SO2 in pigmented samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Bo Liu, Wentao Wang, Tie Gao, Lu Huang, Huihong Fan, Hong-Xu Chen
Summary: Cobratide, a peptide drug extracted from Chinese cobra venom, has shown potential in treating not only chronic pain but also COVID-19. A protocol combining sheathless CE-MS and CE-UV methods was established for the separation, identification, and quantification of cobratide and its impurities, providing valuable information for quality control and process analysis of peptide drugs.
ANALYTICAL METHODS
(2021)
Article
Medicine, General & Internal
Katarzyna Pauter, Viorica Railean-Plugaru, Michal Zloch, Pawel Pomastowski, Malgorzata Szultka-Mlynska, Boguslaw Buszewski
Summary: The study aimed to characterize biofilm before and after antibiotic treatment using methods such as 16S rRNA gene sequencing and capillary electrophoresis. Results showed that antibiotics (amoxicillin and gentamicin) can alter the surface charge of bacterial cells, leading to cell clumping. Additionally, microscopic and spectrometry studies revealed that antibiotics inhibit cell wall synthesis and ribosomal protein synthesis in bacterial cells.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Food Science & Technology
Yu-Min Lee, Shinyoung Lee, Hae-Yeong Kim
Summary: With increased consumption of seafood products, there have been continuous reports of food fraud involving fish resources, particularly codfish. A multiplex PCR method was developed in this study to simultaneously identify five cod species within Gadidae, successfully applied to 40 commercial food products. This detection method will efficiently manage seafood authentication by analyzing multiple cod species simultaneously.
Review
Biochemical Research Methods
Ziting Gao, Wenwan Zhong
Summary: The review focuses on representative works published in 2018-2020 on sample preconcentration, separation media development, detector construction, and multidimensional separation in capillary electrophoresis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Junyi Chen, Briana L. Hickey, Linlin Wang, Jiwon Lee, Adam D. Gill, Alessia Favero, Roberta Pinalli, Enrico Dalcanale, Richard J. Hooley, Wenwan Zhong
Summary: The study demonstrates a method using an array of synthetic hosts and dyes for fluorescence detection of nucleic acid secondary structures, enabling quick differentiation and classification of G-quadruplex structures. By employing a simple multivariate analysis approach, different G-quadruplex structures with similar topological types but different lengths can be identified, making it a simple and widely applicable non-invasive sensing method.
Article
Chemistry, Multidisciplinary
Junyi Chen, Adam D. Gill, Briana L. Hickey, Ziting Gao, Xinping Cui, Richard J. Hooley, Wenwan Zhong
Summary: An arrayed host:guest fluorescence sensor system can accurately discriminate and classify multiple different noncanonical DNA structures through selective molecular recognition. The sensor is highly selective and can distinguish between folds with subtle differences, such as native G-quadruplexes and those with bulges or vacancies. By forming heteroternary complexes with DNA strands, the host mediates the interaction between DNA and dye to modulate emission, and machine learning algorithms enable high-fidelity prediction of the folding state of unknown DNA strands.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Chemistry, Multidisciplinary
Wenwan Zhong, Richard J. Hooley
Summary: Water-soluble, self-folding deep cavitands can overcome the trade-off between selectivity and scope in traditional macrocyclic host-based biosensing. These hosts have flexible structures and large cavities that can bind to various fluorescent dyes, causing fluorescence enhancement or quenching upon binding. By utilizing the affinity of the hosts for NMe3+ groups, site-selective recognition of post-translational lysine methylations can be achieved. Additionally, these hosts can selectively recognize serine phosphorylation, lysine acetylation, and arginine citrullination through non-cavity-based interactions with charged biomolecules.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Review
Biochemical Research Methods
Junyi Chen, Richard J. Hooley, Wenwan Zhong
Summary: Synthetic receptors are powerful tools for molecular recognition, with high selectivity and affinity, tunable and diversified structures, relatively low cost, and simplicity in synthesis and modification. They are feasible for array-based molecular analysis to improve selectivity in recognizing a wide range of targets.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Cell Biology
Minghui Cao, Roi Isaac, Wei Yan, Xianhui Ruan, Li Jiang, Yuhao Wan, Jessica Wang, Emily Wang, Christine Caron, Steven Neben, Denis Drygin, Donald P. Pizzo, Xiwei Wu, Xuxiang Liu, Andrew R. Chin, Miranda Y. Fong, Ziting Gao, Kaizhu Guo, Oluwole Fadare, Richard B. Schwab, Yuan Yuan, Susan E. Yost, Joanne Mortimer, Wenwan Zhong, Wei Ying, Jack D. Bui, Dorothy D. Sears, Jerrold M. Olefsky, Shizhen Emily Wang
Summary: Breast cancer-derived extracellular vesicles suppress pancreatic insulin secretion, leading to dysregulated glucose homeostasis and enhanced tumor growth by targeting PKM in beta-cells.
NATURE CELL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Roxana Coreas, Carmen Castillo, Zongbo Li, Dong Yan, Ziting Gao, Junyi Chen, Dimitrios Bitounis, Dorsa Parviz, Michael S. Strano, Philip Demokritou, Wenwan Zhong
Summary: This study assessed the toxic effects of reduced graphene oxide (rGO) on mammary epithelial cells and found that rGO dissolved in sodium cholate may influence cellular toxicity. Surface-modified c-rGO exhibited higher toxicity, oxidative stress, and mitochondrial disruption compared to rGO, indicating a strong correlation between dispersant coating and cellular impacts.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Engineering, Environmental
Glen M. DeLoid, Xiaoqiong Cao, Roxana Coreas, Dimitrios Bitounis, Dilpreet Singh, Wenwan Zhong, Philip Demokritou
Summary: Despite the lack of knowledge on the health risks and impact on nutrient digestion and absorption of ingested micro-nanoplastics, this study found that the presence of polyethylene incineration-generated nanoparticles increased fat digestion and absorption. The analysis revealed a potential mechanism of triacylglycerol digestion by lipase on the surface of lipid-coated nanoparticles. Further research is needed to explore the underlying mechanisms and verify the results in animal models.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jesus Beltran, Paul J. Steiner, Matthew Bedewitz, Shuang Wei, Francis C. Peterson, Zongbo Li, Brigid E. Hughes, Zachary Hartley, Nicholas R. Robertson, Angelica V. Medina-Cucurella, Zachary T. Baumer, Alison C. Leonard, Sang-Youl Park, Brian F. Volkman, Dmitri A. Nusinow, Wenwan Zhong, Ian Wheeldon, Sean R. Cutler, Timothy A. Whitehead
Summary: This study presents a method for constructing biosensors using the PYR1 plant receptor and successfully evolves sensors with different sensitivities for various small molecules. The PYR1 receptor can be applied to different ligand-responsive outputs, demonstrating high sensitivity.
NATURE BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Junyi Chen, Briana L. Hickey, Ziting Gao, Alexie Andrea P. Raz, Richard J. Hooley, Wenwan Zhong
Summary: An arrayed host:guest fluorescence sensor system can differentiate DNA G-quadruplex structures with minor oxidation or methylation modifications in the guanine base, which cannot be detected by circular dichroism (CD). This sensor system can function in diluted serum and is capable of distinguishing individual modifications in DNA mixtures, providing a powerful method for detecting folding changes caused by DNA damage.
Article
Nanoscience & Nanotechnology
Qiaoshi Jiang, Yuchen Xiao, Anh N. Hong, Ziting Gao, Yuyang Shen, Qingsong Fan, Pingyun Feng, Wenwan Zhong
Summary: The bimetallic Fe/Co-MIL-88(NH2) MOF displays high peroxidase activity, enabling sensitive detection of extracellular vesicles. It serves as a valuable tool in clinical assays for disease diagnosis and prognosis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Ziting Gao, Zachary Hutchins, Zongbo Li, Wenwan Zhong
Summary: This study reports the offline coupling of asymmetrical flow field-flow fractionation (AF4) and capillary electrophoresis (CE) for extracellular vesicle (EV) analysis. AF4 provides gentle and fast separation of EVs by size, while CE resolves EVs from contaminants with similar sizes but different surface charges. The effectiveness of the AF4-CE system in EV analysis was demonstrated through various experiments.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Roxana Coreas, Zongbo Li, Junyi Chen, Wenwan Zhong
Summary: This study assessed the toxicity of tungsten disulfide (WS2) nanosheets and found that low doses induced cell apoptosis, while high doses caused cell necrosis. Furthermore, low-dose exposure led to reduced cell metabolic activity, induction of lipid peroxidation in cell membranes, and uncoupling of mitochondrial oxidative phosphorylation, resulting in the loss of ATP production. The differences in responses to WS2 exposure between normal and tumorigenic cells were also observed, with normal cells experiencing more adverse effects and taking up more nanosheets.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zongbo Li, Yuyang Shen, Jesus Beltran, Hao Tian, Matthew Bedewitz, Ian Wheeldon, Timothy A. Whitehead, Sean R. Cutler, Wenwan Zhong
Summary: The development of a novel sensing platform using magnetic nanorods coated with zwitterionic polymers enables simple, rapid, and sensitive detection of cannabinoids in biofluids. The technique utilizes engineered derivatives as drug recognition elements and employs the chemical-induced dimerization mechanism for signal development. The magnetic nanorods facilitate efficient target capture and the zwitterionic polymer coating exhibits excellent antifouling capability, resulting in excellent sensor performance in complex biofluids.
Article
Chemistry, Multidisciplinary
Briana L. Hickey, Junyi Chen, Yunfan Zou, Adam D. Gill, Wenwan Zhong, Jocelyn G. Millar, Richard J. Hooley
Summary: An arrayed combination of water-soluble deep cavitands and cationic dyes has been shown to optically sense insect pheromones at micromolar concentration in water. Machine learning approaches were used to optimize the most effective array components, which allows differentiation between small structural differences in targets, including between different diastereomers, even though the pheromones have no innate chromophore. When combined with chiral additives, enantiodiscrimination is possible, dependent on the size and shape of the pheromone.
CHEMICAL COMMUNICATIONS
(2021)
