Article
Environmental Sciences
Jeremy P. Koelmel, Elizabeth Z. Lin, Emily Parry, Paul Stelben, Emma E. Rennie, Krystal J. Godri Pollitt
Summary: Non-targeted PFAS analysis was applied to human blood to understand the sources, concentrations, and toxicity of these compounds. High-resolution tandem mass spectrometry and software workflow were used for PFAS characterization in dried blood spots. A total of 28 PFAS and 86 potential PFAS were detected, providing insights for environmental epidemiology studies and policy-making.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Isabel Ten-Domenech, Alvaro Solaz-Garcia, Inmaculada Lara-Canton, Alejandro Pinilla-Gonzalez, Anna Parra-Llorca, Maximo Vento, Guillermo Quintas, Julia Kuligowski
Summary: The GSH-to-GSSG ratio is critical for intracellular redox status. A new LC-MS method for measuring GSH and GSSG in DBS samples was developed, providing a convenient alternative to liquid blood samples. The levels of GSH-NEM and GSSG measured in DBS samples were comparable to those obtained from liquid blood samples in clinical scenarios.
Review
Biochemical Research Methods
Helena Beatriz Ferreira, Ines M. S. Guerra, Tania Melo, Hugo Rocha, Ana S. P. Moreira, Artur Paiva, M. Rosario Domingues
Summary: Dried blood spots (DBS) are being considered as an alternative method for blood collection in lipidomic and other omic analysis. While DBS are widely used in clinical settings for specific measurements, their application in lipidomic studies is still limited. The combination of DBS with lipidomic approaches has shown high sensitivity and specificity, making it a useful tool for biomarker identification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Benjamin C. Moeller, Zicheng Yang
Summary: Research indicates that using dried blood spots (DBS) as an alternative sampling method is feasible for controlling the abuse of prohibited substances such as anabolic steroids. The 11-minute liquid chromatography-mass spectrometry method developed for equine blood samples from DBS cards showed reproducible results and good stability after storage at ambient temperatures for almost 3 months. Therefore, DBS may be an effective alternative sampling approach in equine drug testing.
DRUG TESTING AND ANALYSIS
(2021)
Article
Chemistry, Analytical
C. Louwrens Braal, Mei H. Lam, Tineke Rienks, Claudia J. van Tilborg, Wendy Heuts, Joan B. Heijns, Monique E. M. M. Bos, Ron H. J. Mathijssen, Peter de Bruijn, Stijn L. W. Koolen
Summary: A reliable and specific LC-MS/MS method was developed for determining ribociclib in both DBS samples and plasma. The method met FDA guidelines for validation and showed a strong correlation between ribociclib concentrations in DBS and plasma. This study demonstrates the potential of using DBS for monitoring ribociclib levels in breast cancer patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Michel Boutin, Pamela Lavoie, Margot Beaudon, Georges Kabala Ntumba, Daniel G. G. Bichet, Bruno Maranda, Christiane Auray-Blais
Summary: Fabry disease is an X-linked lysosomal storage disorder characterized by impaired alpha-galactosidase A enzyme activity. The accumulation of undegraded glycosphingolipids, including lyso-Gb(3) and related analogues, is a useful biomarker for screening and longitudinal patient evaluation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Michal Kopcil, Roman Kand'ar
Summary: Uric acid and its oxidation product allantoin serve as excellent biomarkers for oxidative stress in humans. A fast, sensitive, and reliable HPLC-MS/MS method was developed for the simultaneous determination of uric acid and allantoin from dried blood spots. The method utilizes stable isotopically labeled analogs as internal standards and can provide accurate results within a short analysis time. This method is suitable for screening and monitoring allantoin and uric acid levels as biomarkers of oxidative stress in clinical practice.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Sing Teang Kong, Hai-Shu Lin, Jianhong Ching, Huiqing Xie, Paul C. Ho
Summary: This study profiled the metabolic changes in rats treated with valproate and found subtle differences in the metabolic changes between males and females. The use of dried blood spots as sample matrices proved to be a powerful tool for monitoring drug levels and metabolic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Yuling Yuan, Youxuan Xu, Jianghai Lu
Summary: The development of DBS technology has made doping detection more convenient and cost-effective. Future research should focus on aspects such as sample collection, storage, pretreatment, instrumentation, and validation in DBS, in order to further improve the accuracy and efficiency of doping detection.
Article
Environmental Sciences
Emily K. Griffin, Juan J. Aristizabal-Henao, John A. Bowden
Summary: PFAS are environmental contaminants associated with adverse health effects in humans and wildlife. Marine mammals, particularly manatees, are considered sentinels for assessing the presence and effects of anthropogenic chemicals. The study evaluated four different methods for extracting PFAS from dried blood spots.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Biochemical Research Methods
Casey Ward, Shriram Nallamshetty, Jeramie D. Watrous, Eowyn Acres, Tao Long, Ian T. Mathews, Sonia Sharma, Susan Cheng, Farhad Imam, Mohit Jain
Summary: Advances in high-resolution, nontargeted mass spectrometry allow for simultaneous measurement of thousands of metabolites in a single biosample, while dried blood spotting offers a simple approach to overcome barriers in human blood acquisition and storage. Over 4,400 spectral features can be efficiently extracted from human dried blood spots, with over 80% of metabolites found to be chemically stable when stored at room temperature for up to a week.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Environmental Sciences
Elizabeth Z. Lin, Sara L. Nason, Alexander Zhong, John Fortner, Krystal J. Godri Pollitt
Summary: Per- and polyfluoroalkyl substances (PFAS) are man-made chemicals widely used in consumer products for their stain- and water-repellent properties. PFAS exposure has been linked to adverse health outcomes. Dried blood spots (DBS) have gained attention as a biomatrix for PFAS exposure assessment due to their easy collection and storage. This study developed and validated an analytical method to measure PFAS in DBS, which showed high recovery and reproducibility compared to liquid whole blood samples. DBS can provide novel insights into environmental exposures, especially during vulnerable periods.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Chemistry, Analytical
Hanne Bendiksen Skogvold, Helge Rootwelt, Leon Reubsaet, Katja Benedikte Presto Elgstoen, Steven Ray Wilson
Summary: Dried blood spot samples offer a simple and safe option for diagnostics, with liquid chromatography-mass spectrometry being a versatile measurement tool. These samples provide valuable information for metabolomics, xenobiotic analysis, and proteomics. Targeted analysis of small molecules is the main application, but there are emerging applications in untargeted metabolomics and proteomics. Applications range from newborn screening to disease diagnostics and monitoring, as well as research into the effects of diet, exercise, xenobiotics, and doping. A variety of dried blood spot products and liquid chromatography-mass spectrometry methods are available, including novel approaches like on-paper sample preparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Travis M. Falconer, Birgit Schneider, Carsten Baessmann, Karin Wendt, Artem Filipenko
Summary: The use of trapped ion mobility spectrometry (TIMS) in conjunction with liquid chromatography (LC) and electrospray ionization tandem mass spectrometry (MS/MS) was explored for the analysis of PDE-5 inhibitor analogs. Six out of eight compounds were resolved by LC and TIMS, while the remaining two isomers were distinguished by unique product ions in the MS/MS spectrum. The LC separation corresponded to differences in substitution on the piperazine moiety of the PDE-5 inhibitors, while the TIMS separation corresponded to the position of a nitrogen atom in the fused ring region of the molecules.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Endocrinology & Metabolism
Peiling Su, Hamid Khaledi, Christine Waggoner, Michael H. Gelb
Summary: GM1-gangliosidosis may require screening in newborns in the future due to ongoing gene therapy clinical trials. A new enzymatic assay method was utilized to show significantly lower GLB1 activities in newborn DBS from GM1-gangliosidosis patients compared to random samples, as well as high levels of glycan biomarkers dp5 and A2G2 in affected newborns.
JOURNAL OF INHERITED METABOLIC DISEASE
(2021)
