Article
Engineering, Biomedical
Da Ying, Joshua Rosenberg, Naveen K. Singh, Drew A. Hall
Summary: This paper presents an analog front-end (AFE) for fast-scan cyclic voltammetry (FSCV) that uses a pseudo-differential sensing scheme to cancel non-faradaic current and achieve a more compact and low-power design. The AFE achieves excellent performance in experimental tests.
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2022)
Article
Chemistry, Multidisciplinary
Yumin Kang, Abhinav Goyal, Sangmun Hwang, Cheonho Park, Hyun U. Cho, Hojin Shin, Jinsick Park, Kevin E. Bennet, Kendall H. Lee, Yoonbae Oh, Dong Pyo Jang
Summary: FSCV is a technique for measuring neurotransmitter release with millisecond temporal resolution, capable of measuring concentrations down to the nanomolar scale. By optimizing the technique, the detection limit of FSCV can be further lowered.
Article
Chemistry, Multidisciplinary
Mimi Shin, B. Jill Venton
Summary: In this study, we developed fast-scan cyclic voltammetry (FSCV) to measure dopamine release in fruit flies during behavior. The results showed that sugar feeding induced longer dopamine release compared to acetylcholine stimulation in the mushroom body. In addition, administering dopamine transporter inhibitor or D2 receptor antagonist significantly increased sugar-evoked dopamine release.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Cell Biology
Vladimir P. Grinevich, Amir N. Zakirov, Uliana V. Berseneva, Elena V. Gerasimova, Raul R. Gainetdinov, Evgeny A. Budygin
Summary: Fast-scan cyclic voltammetry (FSCV) is a technique used for real-time monitoring of neurotransmitter dynamics, which has significant implications in the study of neuropsychiatric disorders.
Article
Chemistry, Analytical
Michelle Hadad, Nadine Hadad, Alexander G. Zestos
Summary: In this study, a method for measuring cortisol using carbon fiber microelectrodes and fast-scan cyclic voltammetry was developed. This method showed high sensitivity, high spatiotemporal resolution, and biocompatibility, and it was able to detect cortisol stably. Its application will contribute to a better understanding of the physiological importance of cortisol.
Article
Biochemical Research Methods
Noel Manring, Muzammil M. N. Ahmed, Jessica L. Smeltz, Pavithra Pathirathna
Summary: The etiology of neurodegenerative diseases is poorly understood and the heavy metal copper has been found to play a critical role in neurotoxicity, affecting the development of these diseases. Analyzing heavy metals in the brain is challenging due to the limitations of existing metal detection tools, resulting in inadequate data for developing efficient therapeutics. This study developed a surface-modified electrode using fast-scan cyclic voltammetry to detect ultra-low concentrations of copper in the brain accurately and in real time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rohan Bhimani, Ryan Yates, Caroline E. Bass, Jinwoo Park
Summary: The olfactory tubercle (OT) is an important brain region involved in the integration of reward-related information, with its subregions showing different roles in dopamine regulation. Studies have found that VTA-DA regulation in the medial OT is less dependent on dopamine transporters and has greater dopamine transmission than the lateral OT.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Electrochemistry
Chase Stucky, Michael A. Johnson
Summary: In this study, it was found that three different SSRIs significantly decrease the oxidation current of serotonin when using the commonly used Jackson waveform. Coating carbon-fiber microelectrodes with Nafion further reduces the current and increases response times. To address fouling issues, an extended serotonin waveform was used, which alleviated the decrease in current, indicating reduced electrode fouling.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Anna C. Everett, Ben E. Graul, Joakim W. Ronstrom, J. Kayden Robinson, Daniel B. Watts, Rodrigo A. Espana, Cody A. Siciliano, Jordan T. Yorgason
Summary: This study presents an alternative approach for measuring and distinguishing dopamine release from clearance using the first derivative of evoked dopamine signals. The maximal upward velocity predicts reductions in dopamine peak height, while the Michaelis-Menten maximal velocity measure predicts maximal downward velocity. Various measures can be used to assess dopamine release and clearance based on the experimental results.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Chemistry, Analytical
Edoardo Cuniberto, Zhujun Huang, Michael D. Ward, Davood Shahrjerdi
Summary: Fast-scan cyclic voltammetry (FSCV) with micron-sized carbon sensors is a promising method for monitoring serotonin (5-HT) neuromodulatory signals in the brain. However, the complex chemical reactions associated with 5-HT can compromise sensor performance. This study used engineered graphitic sensors to characterize the electrochemistry of 5-HT and optimize the FSCV conditions for quantitative in vivo detection. The results revealed the significant role of water in driving side reactions and provided new insights for more reliable 5-HT detection.
Article
Biophysics
Elisa Castagnola, Raghav Garg, Sahil K. Rastogi, Tzahi Cohen-Karni, Xinyan Tracy Cui
Summary: The newly developed three-dimensional fuzzy graphene microelectrode arrays show high sensitivity and stability for dopamine detection, enabling multi-site detection in the brain and potentially improving treatments and understanding of neurological disorders.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Samuel Rantataro, Laura Ferrer Pascual, Tomi Laurila
Summary: It is widely believed that ascorbic acid (AA) interferes with the detection of neurotransmitters using electrochemical methods. However, this study shows that AA concentration decreases rapidly in cell culture medium, eventually becoming undetectable by electrodes, allowing for effective monitoring of neurotransmitter concentrations.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Romana Jarosova, Blaise J. Ostertag, Ashley E. Ross
Summary: In this study, we synthesized and characterized graphene oxide (GO) fiber microelectrodes with controllable nanosheet orientation to investigate the impact of sheet alignment on electrochemical detection of neurochemicals. The alignment of GO nanosheets was characterized using scanning electron microscopy, Raman spectroscopy, and cyclic voltammetry. The electrochemical performance and suitability of GO microelectrodes for rapid detection of neurotransmitters were evaluated using fast-scan cyclic voltammetry (FSCV). The results showed that the alignment of GO sheets significantly affects electron transfer kinetics, frequency independent behavior, and suitability for specific neurotransmitter detection. Therefore, this fine-tuning aspect of electrode surface is crucial for future applications of GO-based biological sensors.
Article
Chemistry, Multidisciplinary
Romana Jarosova, Blaise J. Ostertag, Ashley E. Ross
Summary: In this study, graphene oxide (GO) fiber microelectrodes with controllable nanosheet orientation were synthesized and characterized to investigate the impact of sheet alignment and orientation on electrochemical detection of neurochemicals. The results showed that the alignment of GO nanosheets significantly influenced the electron transfer kinetics, frequency independent behavior, and detection suitability for specific neurotransmitters.
Article
Biochemistry & Molecular Biology
Piyanka Hettiarachchi, Michael A. Johnson
Summary: This study examines the function of D3 autoreceptors in regulating dopamine release and uptake in zebrafish. The results show that D3 autoreceptors modulate dopamine release, likely by inhibiting uptake. These findings are important for the further development of zebrafish as a model organism for studying dopamine-related neurological disorders.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
S. E. Calhoun, C. J. Meunier, C. A. Lee, G. S. McCarty, L. A. Sombers
ACS CHEMICAL NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Alexander Gomez-A, Tatiana A. Shnitko, Haley M. Barefoot, Eleanor L. Brightbill, Leslie A. Sombers, Saleem M. Nicola, Donita L. Robinson
ACS CHEMICAL NEUROSCIENCE
(2019)
Article
Chemistry, Analytical
Carl J. Meunier, Gregory S. McCarty, Leslie A. Sombers
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
James G. Roberts, Edwin C. Mitchell, Lars E. Dunaway, Gregory S. McCarty, Leslie A. Sombers
Article
Chemistry, Multidisciplinary
Carl J. Meunier, J. Dylan Denison, Gregory S. McCarty, Leslie A. Sombers
Article
Biochemical Research Methods
Alexandra G. Forderhase, Hannah C. Styers, Christie A. Lee, Leslie A. Sombers
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Richard B. Keithley, Dylan T. Sullivan, John M. Dodd, Kavya V. Iyer, Catherine A. Sarisky, Timothy W. Johann
Summary: A laboratory series has been developed using student-built inexpensive fluorometers for kinetic analysis of lactate dehydrogenase, providing biochemistry students with practical exposure to fluorescence fundamentals. Assessment of learning outcomes indicates proficient understanding of instrument use and design.
JOURNAL OF CHEMICAL EDUCATION
(2021)
Editorial Material
Biochemistry & Molecular Biology
Leslie A. Sombers, Heather B. Patisaul
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Chemistry, Analytical
Gregory S. McCarty, Lars E. Dunaway, J. Dylan Denison, Leslie A. Sombers
Summary: This study investigates the reasons why released neurotransmitters can escape detection in amperometric measurements. The results reveal that both diffusional loss and the action of the norepinephrine transporter contribute to the undetected neurotransmitter. This has important implications for understanding the mechanisms of neurotransmitter release.
ANALYTICAL CHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Alexandra G. Forderhase, Laney C. Kimble, Leslie A. Sombers
Summary: After two postponements due to the global COVID-19 pandemic, the 18th Biennial Monitoring Molecules in Neuroscience (MMiN) Research Conference was held in Lyon, France, in late June 2022, with around 200 scientists in attendance. Despite unprecedented challenges in coordination, the conference was a huge success, providing young neuroscientists with a valuable opportunity to network and learn about advancements in molecular monitoring in neuroscience research. The topics discussed ranged from improvements in established analytical techniques to the development of novel methods, including multiplexed detection of neurochemicals, established sensors utilizing enzymes and other biological components, and the creation of diverse, customizable genetically encoded sensors.
ACS CHEMICAL NEUROSCIENCE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Leslie A. Sombers
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
J. Dylan Denison, A. Chathuri De Alwis, Ruby Shah, Gregory S. Mccarty, Leslie A. Sombers
Summary: This study used a planar carbon-fiber microelectrode as a sensor to detect the release dynamics of catecholamine and opioid neuropeptides from chromaffin cells. The data revealed unique kinetics for these two neurochemical classes at the single-vesicle level. The study provides unprecedented insight into key differences between catecholamine and opioid neuropeptide release from isolated chromaffin cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
A. Chathuri De Alwis, J. Dylan Denison, Ruby Shah, Gregory S. McCarty, Leslie A. Sombers
Summary: Carbon fiber microelectrodes are commonly used for real-time monitoring of exocytosis events. This study compared the performance of 10-μm and 34-μm diameter microelectrodes and found that the larger electrode was more sensitive to certain molecules and covered the entire cell, leading to better detection of exocytosis events and quantal size.
Article
Chemistry, Multidisciplinary
Laney C. Kimble, Jack S. Twiddy, Jenna M. Berger, Alexandra G. Forderhase, Gregory S. Mccarty, John Meitzen, Leslie A. Sombers
Summary: This article presents a novel glutamate microbiosensor fabricated on a carbon-fiber microelectrode substrate and coupled with fast-scan cyclic voltammetry (FSCV) for simultaneous quantification of glutamate and dopamine in the brain. The sensor demonstrates high sensitivity, stability, and selectivity, making it a powerful tool for studying neural circuits.
Meeting Abstract
Chemistry, Multidisciplinary
Catherine Sarisky, Tim Johann, Kelly Anderson, William Brenzovich, Emily Hardy, W. Hollis, Steven Hughes, Jemima Ingle, Richard Keithley, Steven Kennedy, Stephanie Livingston
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)