☆ 4.8 Article

Confocal, Three-Dimensional Tracking of Individual Quantum Dots in High-Background Environments

ANALYTICAL CHEMISTRY (2008)

期刊

ANALYTICAL CHEMISTRY

卷 80, 期 24, 页码 9830-9834

出版社

AMER CHEMICAL SOC

DOI: 10.1021/ac8021899

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Chemistry, Analytical

资金

National Institutes of Health [R21AI07707]
National Nanotechnology Enterprise Development Center (NNEDC)
U.S. Department of Energy [DE-AC52-06NA25396]

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摘要

We demonstrate a custom confocal fluorescence-microscope that is capable of tracking individual quantum dots undergoing three-dimensional Brownian motion (diffusion coefficient similar to 0.5 mu m(2)/s) in environments with a signal-to-background ratio as low as 2: 1, significantly worse than observed in a typical cellular environment. By utilizing a pulsed excitation source and time-correlated single photon counting, the time-resolved photon stream can be used to determine changes in the emission lifetime as a function of position and positively identify single quantum dots via photon-pair correlations. These results indicate that this microscope will be capable of following protein and RNA transport throughout the full three-dimensional volume of a live cell for durations up to 15 s.

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