Article
Biochemical Research Methods
Pauline Legrand, Oumar Dembele, Helena Alamil, Catherine Lamoureux, Nathalie Mignet, Pascal Houze, Rabah Gahoual
Summary: The translation text describes an analytical strategy that enables the structural identification and quantification of monoclonal antibodies in potentially counterfeit drugs. By using capillary zone electrophoresis analysis, size exclusion chromatography coupled to multi-angle light scattering, and liquid chromatography tandem mass spectrometry, information on the nature and structure of mAbs is obtained.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Yang Wen, Anguo Liu, Chengzhen Meng, Zhen Li, Pingli He
Summary: A sensitive method for quantifying lectin in soybean samples was developed, with a detection limit of 35.5 μg/g. The method was successfully applied to evaluate lectin levels in different varieties of soybean seeds and soy products, showing potential as a general method for detecting various protein anti-nutritional factors in food.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Xiaoxi Zhang, Teresa Kwok, Mike Zhou, Min Du, Victor Li, Tao Bo, Tiemin Huang, Tong Chen
Summary: Since the development of the first commercial imaged capillary isoelectric focusing (icIEF) instrument twenty years ago, this technology has become the gold standard for quality and manufacturing process control in the bio-pharmaceutical industry. This study presents a robust and flexible icIEF-MS platform that allows for rapid icIEF separation and high-resolution MS identification of protein charged variants simultaneously.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Ramesh Kumar, Rohan L. Shah, Shadab Ahmad, Anurag S. Rathore
Summary: This study introduces the use of an LC-CE-MS/MS platform for the analysis of host cell proteins (HCPs), which has shown to successfully identify a greater number of HCPs compared to current LC-MS/MS and CE-MS/MS techniques. The platform combines the benefits of both LC-MS/MS and CE-MS/MS, allowing for identification of peptides in a wider range of properties and providing more insights into cellular components and functions.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Shengnan Ji, Xiaomeng Liu, Jing Ha, Lianfeng Ai, Zheng Li
Summary: A sensitive and simple LC-MS/MS method was developed for the determination of orelabrutinib in human plasma and cerebrospinal fluid. The method involved simple protein precipitation, efficient separations, and optimized mass spectrometric conditions to increase selectivity and sensitivity. The method was validated for accuracy, precision, selectivity, linearity, recovery, matrix effect, stability, and limits of quantification. It exhibited a lower limit of quantification of 0.50 ng/mL, with intraday and interday precision RSD both less than 15%, and a recovery rate of 85.7%-92.9%. The method was successfully applied for quantitation in clinical patients treated with orelabrutinib.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Patrik Matlak, Hana Brozmanova, Pavel Sistik, Ivana Kacirova, Pavel Hradilek, Milan Grundmann
Summary: This study aimed to develop a LC-MS/MS method for quantifying natalizumab in human serum and cerebrospinal fluid (CSF) and applying it to clinical settings. By analyzing specific peptide sequences of natalizumab, the concentration was successfully determined. The method met all accuracy and precision acceptance criteria according to the EMA guideline and is suitable for clinical applications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Engineering, Chemical
Chang-Seob Seo, Hyeun-Kyoo Shin
Summary: In this study, a traditional herbal formula called Sogunjung decoction was quantified using LC-MS, and a quality-control protocol was developed for it. The validated method detected 11 marker components in the decoction, with amounts ranging from 0.01 to 51.83 mg/g.
Article
Biochemistry & Molecular Biology
Ann-Kathrin Weishaupt, Laura Kubens, Lysann Ruecker, Tanja Schwerdtle, Michael Aschner, Julia Bornhorst
Summary: Neurotransmitters like dopamine, serotonin, GABA, and acetylcholine have a crucial role in transmitting excitation between neurons in the central nervous system. Imbalance in neurotransmitter levels is associated with neurological disorders, making it important to accurately assess and target neurotransmitter levels. The model organism C. elegans, along with a validated LC-MS/MS method, provides a powerful tool for studying neurotoxicity mechanisms and identifying potential therapies.
Article
Biochemistry & Molecular Biology
Jakob Morgenstern, Thomas Fleming, Elisabeth Kliemank, Maik Brune, Peter Nawroth, Andreas Fischer
Summary: This study developed a sensitive and specific method to quantify all-trans retinoic acid (atRA) in human plasma samples, which can also be applied to hyperlipidemic samples. The method showed significant differences in atRA plasma levels between patients with different types of diseases, and the concentration of atRA was associated with lipoprotein levels when compared to healthy controls.
Article
Biochemical Research Methods
Chun-Yue Han, Li Ge, Chi Zhang, Li-Na Ding, Peng Wang, Fang Yu, Sheng Wang, Lili Zhu, Qunlin Zhang, Qi Liu, Fei-Long Liu
Summary: In this study, a pair of diazo probes, 2-DMBA and d5-2-DMBA, were used to label amino acids, resulting in enhanced ionization efficiencies during LC-MS analysis. The developed method enabled the sensitive and accurate detection of 17 amino acids in microliter level of serum samples, revealing differences in amino acid contents between normal and B16F10-tumour mice. This chemical labeling-LC-MS analysis provides a potentially valuable tool for investigating the link between amino acid metabolism and diseases.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Tessa Reinert, Rabah Gahoual, Nathalie Mignet, Alexandre Kulus, Matthieu Allez, Pascal Houze, Yannis-Nicolas Francois
Summary: Monoclonal antibodies have shown great success in therapeutic areas such as oncology and immune disorders. However, there is limited understanding of their structural evolution after administration, and significant inter-patient differences in clearance and clinical responses have been observed.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Genetics & Heredity
J. Isaacson, E. Schwoebel, A. Shcherbina, D. Ricke, J. Harper, M. Petrovick, J. Bobrow, T. Boettcher, B. Helfer, C. Zook, E. Wack
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2015)
Article
Rheumatology
D. M. Urech, U. Feige, S. Ewert, V. Schlosser, M. Ottiger, K. Polzer, G. Schett, P. Lichtlen
ANNALS OF THE RHEUMATIC DISEASES
(2010)
Article
Hematology
Alexander W. Koch, Nikolaus Schiering, Samu Melkko, Stefan Ewert, Janeen Salter, Yiming Zhang, Peter McCormack, Jianying Yu, Xueming Huang, Yu-Hsin Chiu, Zhiping Chen, Simone Schleeger, Geraldine Horny, Keith DiPetrillo, Lionel Muller, Andreas Hein, Frederic Villard, Meike Scharenberg, Paul Ramage, Ulrich Hassiepen, Serge Cote, Julie DeGagne, Carsten Krantz, Jorg Eder, Brian Stoll, Kenneth Kulmatycki, David L. Feldman, Peter Hoffmann, Craig T. Basson, Robert J. A. Frost, Yasser Khder
Article
Medicine, Legal
Darrell O. Ricke, Philip Fremont-Smith, James Watkins, Sara Stankiewicz, Tara Boettcher, Eric Schwoebel
JOURNAL OF FORENSIC SCIENCES
(2019)
Article
Biochemical Research Methods
Ge Liu, Haoyang Zeng, Jonas Mueller, Brandon Carter, Ziheng Wang, Jonas Schilz, Geraldine Horny, Michael E. Birnbaum, Stefan Ewert, David Gifford
Article
Medicine, Legal
Darrell O. Ricke, James Watkins, Philip Fremont-Smith, Martha S. Petrovick, Tara Boettcher, Eric Schwoebel
Summary: This study explores the complexities of analyzing complex forensic DNA mixtures using MPS SNP panels, and introduces the concept of creating paired derived mixtures with increased analytical thresholds for more accurate analysis. The proposed TranslucentID tool has shown promising results in identifying multiple individuals in mixtures with large numbers of donors.
AUSTRALIAN JOURNAL OF FORENSIC SCIENCES
(2021)
Article
Medicine, Research & Experimental
Assunta Senatore, Karl Frontzek, Marc Emmenegger, Andra Chincisan, Marco Losa, Regina Reimann, Geraldine Horny, Jingjing Guo, Sylvie Fels, Silvia Sorce, Caihong Zhu, Nathalie George, Stefan Ewert, Thomas Pietzonka, Simone Hornemann, Adriano Aguzzi
EMBO MOLECULAR MEDICINE
(2020)
Article
Oncology
Haihui Lu, Ryan D. Molony, Dongshu Chen, Sunyoung Jang, Babette Wolf, Stefan Ewert, Meghan Flaherty, Fangmin Xu, Sinan Isim, Yeonju Shim, Christina Dornelas, Nicole Balke, Xavier Charles Leber, Meike Scharenberg, Johanna Koelln, Eugene Choi, Rebecca Ward, Jennifer Johnson, Thomas Calzascia, Isabelle Isnardi, Juliet A. Williams, Pieter L. Lindenbergh, Niels W. C. J. van de Donk, Tuna Mutis, Heather Huet, Emma Lees, Matthew J. Meyer
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Infectious Diseases
Maurice Fremont-Smith, Nicole Gherlone, Nora Smith, Philip Tisdall, Darrell O. Ricke
Summary: The clinical manifestations of COVID-19 associated cardiac complications vary, with localized ischemia and subsequent cell death by anoxia potentially playing a key role in the cardiac pathology of some patients. Vasoconstriction of cardiac pericyte cells induced by elevated histamine from hyper-activated mast cells or direct infection may be a new mechanism leading to cardiac injury in COVID-19 patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Proceedings Paper
Computer Science, Hardware & Architecture
Darrell O. Ricke
2017 IEEE HIGH PERFORMANCE EXTREME COMPUTING CONFERENCE (HPEC)
(2017)
Proceedings Paper
Computer Science, Hardware & Architecture
Siddharth Samsi, Brian Helfer, Jeremy Kepner, Albert Reuther, Darrell O. Ricke
2017 IEEE HIGH PERFORMANCE EXTREME COMPUTING CONFERENCE (HPEC)
(2017)
Proceedings Paper
Engineering, Electrical & Electronic
Ninoshka K. Singh, Darrell O. Ricke
2016 IEEE 13TH INTERNATIONAL CONFERENCE ON WEARABLE AND IMPLANTABLE BODY SENSOR NETWORKS (BSN)
(2016)
Article
Multidisciplinary Sciences
Richa Saxena, Benjamin F. Voight, Valeriya Lyssenko, Noel P. Burtt, Paul I. W. de Bakker, Hong Chen, Jeffrey J. Roix, Sekar Kathiresan, Joel N. Hirschhorn, Mark J. Daly, Thomas E. Hughes, Leif Groop, David Altshuler, Peter Almgren, Jose C. Florez, Joanne Meyer, Kristin Ardlie, Kristina Bengtsson Bostroem, Bo Isomaa, Guillaume Lettre, Ulf Lindblad, Helen N. Lyon, Olle Melander, Christopher Newton-Cheh, Peter Nilsson, Marju Orho-Melander, Lennart Rastam, Elizabeth K. Speliotes, Marja-Riitta Taskinen, Tiinamaija Tuomi, Candace Guiducci, Anna Berglund, Joyce Carlson, Lauren Gianniny, Rachel Hackett, Liselotte Hall, Johan Holmkvist, Esa Laurila, Marketa Sjoegren, Maria Sterner, Aarti Surti, Margareta Svensson, Malin Svensson, Ryan Tewhey, Brendan Blumenstiel, Melissa Parkin, Matthew DeFelice, Rachel Barry, Wendy Brodeur, Jody Camarata, Nancy Chia, Mary Fava, John Gibbons, Bob Handsaker, Claire Healy, Kieu Nguyen, Casey Gates, Carrie Sougnez, Diane Gage, Marcia Nizzari, Stacey B. Gabriel, Gung-Wei Chirn, Qicheng Ma, Hemang Parikh, Delwood Richardson, Darrell Ricke, Shaun Purcell