Article
Biochemistry & Molecular Biology
Olivia D. Walsh, Leona Choi, Krishna P. Sigdel
Summary: Antimicrobial peptides play a crucial role in the immune system and can affect membrane integrity. This study used atomic force microscopy to investigate the effect of a synthetic hybrid peptide CM15 on the lipid bilayer of E. coli. It was found that CM15 quickly formed membrane-spanning defects and disrupted membrane integrity at high concentrations.
Article
Materials Science, Multidisciplinary
Tun Naw Sut, Bo Kyeong Yoon, Won-Yong Jeon, Joshua A. Jackman, Nam-Joon Cho
Summary: Supported lipid bilayer (SLB) coatings, versatile cell-membrane-mimicking biointerfaces, have been expanded for medical and biotechnology applications, facilitating various studies and applications like biosensors, diagnostics, and antifouling surfaces.
APPLIED MATERIALS TODAY
(2021)
Article
Nanoscience & Nanotechnology
Guilherme B. Berselli, Aurelien Gimenez, Alexandra O'Connor, Tia E. Keyes
Summary: A biomolecular photoactive device based on light-activated proton pump bR reconstituted into highly fluidic microcavity-supported lipid bilayers (MSLBs) has been developed, demonstrating efficient photoelectrical properties. The optimal conditions for a self-sustaining photoelectrical switch were determined, showing significant increase in transient current for lipid bilayers containing approximately 0.3 mol % bR with a measured photo-current of 250 nA/cm(2).
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemical Research Methods
Siddhartha Banerjee, Yuri L. Lyubchenko
Summary: The article discusses the preparation of smooth, stable, and structurally homogeneous lipid bilayers without trapped vesicles, for probing the time-dependent early stages of aggregation of monomeric amyloid proteins. This methodology development allows for monitoring amyloid protein aggregation at low protein concentrations, and for studying the specific role of different lipids in aggregation kinetics and disease development mechanisms.
Article
Chemistry, Analytical
Karutha Pandian Divya, Shanmuganathan Keerthana, Chinnuswamy Viswanathan, Nagamony Ponpandian
Summary: This paper reports a biomimetic bilayer lipid membrane supported MXene based biosensor for electrochemical hybridization detection of the most prevalent and potential breast cancer biomarker BRCA1. A 2D MXene nanosheet-anchored gold nanoparticle-decorated biomimetic bilayer lipid membrane (AuNP@BLM) biosensor is used for the attachment of thiolated single-stranded DNA (HS-ssDNA) targeting hybridization detection. The synergistic combination of MXene and AuNP@BLM has proven to efficiently improve the detection signal to several folds.
Article
Nanoscience & Nanotechnology
Hyunhyuk Tae, Soohyun Park, Gamaliel Junren Ma, Nam-Joon Cho
Summary: In this study, an air-stable supported lipid bilayer (SLB) that is laterally mobile upon rehydration was fabricated using a sucrose-bicelle complex system. The sucrose fraction in the system was found to be an important factor, with 20 wt% sucrose yielding SLBs that fully recovered lateral mobility after the dehydration-rehydration process. This research provides potential for a wide range of biointerfacial science applications.
Article
Biophysics
Surajit Ghosh, Zeinab Mohamed, Jung-Ho Shin, Samavi Farnush Bint E. Naser, Karan Bali, Tobias Dorr, Roisin M. Owens, Alberto Salleo, Susan Daniel
Summary: Antibiotic resistance is a global health concern due to the decreasing number of available antibiotics and the development of drug-resistant bacteria. Understanding the interactions between the bacterial membrane and antibiotics is crucial for designing novel antimicrobial drugs. By creating a surface-supported planar bacterial outer membrane model, researchers can study these interactions and gain insights into membrane properties for drug design.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Rashad Kariuki, Rowan Penman, Saffron J. Bryant, Rebecca Orrell-Trigg, Nastaran Meftahi, Russell J. Crawford, Chris F. McConville, Gary Bryant, Kislon Voitchovsky, Charlotte E. Conn, Andrew J. Christofferson, Aaron Elbourne
Summary: Nanomaterials have the potential to revolutionize biological and biomedical research, and this study provides detailed insights into how gold nanoparticles interact with phospholipid membranes. The study shows that the nanoparticles can be absorbed and internalized by fluid-phase membranes, and partially embed into gel-phase membranes, causing perturbations throughout the bilayers.
Article
Biochemistry & Molecular Biology
Kathleen W. Swana, Terri A. Camesano, Ramanathan Nagarajan
Summary: Supported lipid bilayers (SLBs) on quartz crystals are versatile model systems for studying cell membrane behavior. This study successfully formed entirely anionic SLBs by treating the quartz crystal, which provides valuable model systems for studying interactions with Gram-positive bacterial membranes.
Article
Multidisciplinary Sciences
Joyce J. Wong, Zhongwen Chen, Jean K. Chung, Jay T. Groves, Theodore S. Jardetzky
Summary: Paramyxovirus membrane fusion requires an attachment protein binding to a host cell receptor and a fusion protein merging viral and host membranes. The dynamic events of NiV fusion activation involve G and ephrinB2 forming clusters, with ephrinB2 oligomerization being essential for F activation. Single-molecule tracking of F particles revealed an immobilized intermediate upon activation, and no stable F-G protein complexes were found.
Article
Materials Science, Multidisciplinary
Konstantinos Kallitsis, Anna-Maria Pappa, Zixuan Lu, Alberto Alvarez-Fernandez, Ioanna Charalambous, Sina Schack, Walther C. Traberg, Quentin Thiburce, Karan Bali, Graham Christie, Stefan Guldin, Susan Daniel, Alberto Salleo, Roisin M. Owens
Summary: This study presents a versatile method to modify the surface chemistry of conducting polymers, aiming to bridge the chemical gap between bioelectronic devices and biological systems. The surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is modified to enhance the formation of cell-derived supported lipid bilayers (SLBs). The functionalized devices show improved electronic output and demonstrate the potential of this approach in membrane-on-a-chip systems.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Toshinori Motegi, Kingo Takiguchi, Yohko Tanaka-Takiguchi, Toshiki Itoh, Ryugo Tero
Summary: This study explored the size, distribution, and fluidity of microdomains in a lipid bilayer containing phosphatidylinositol (PI), showing that PI-derived submicron domains hindered lipid diffusion. Furthermore, the PI-derived microdomain acted as a scaffold for protein adsorption during the two-dimensional assembly of a membrane deformation protein.
Article
Physics, Applied
Ryugo Tero, Natsumi Kobayashi
Summary: Supported lipid bilayers (SLBs) are artificial lipid bilayers used as cell membrane model systems. This study investigated the effect of electrostatic interaction between the substrate surface and the lipid bilayer on the composition of SLBs. It was found that the lipid composition in SLBs can be controlled by modulating the substrate-induced electrostatic potential.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Nanoscience & Nanotechnology
Elia Roma, Pietro Corsi, Max Willinger, Nikolaus Simon Leitner, Ronald Zirbs, Erik Reimhult, Barbara Capone, Tecla Gasperi
Summary: This study presents a comprehensive approach using experimental, theoretical, and computational methods to design macromolecules capable of adsorbing cargo at the nanoscale. The experimental results validate the successful design process and confirm the accuracy of theoretical predictions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Katharina R. Lenhardt, Hergen Breitzke, Gerd Buntkowsky, Erik Reimhult, Max Willinger, Thilo Rennert
Summary: The study reveals that the structure of SROAS has a significant impact on their aggregation effects and properties, with different compositions of SROAS showing varying degrees of surface loss due to aggregation upon freezing. Cryomilling effectively fractures aggregates but does not change the magnitude of specific surface area, indicating a structure-dependent effect on spatial arrangement at the submicron scale. Understanding the physical and chemical processes of aggregation as a function of composition and crystallinity may enhance our knowledge of the reactivity of SROAS in the environment.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Tomasz Clapa, Jakub Michalski, Anna Syguda, Dorota Narozna, Peter van Oostrum, Erik Reimhult
Summary: Pseudomonas aeruginosa is a multi-drug resistant pathogen classified as one of the most life-threatening pathogens. Ionic liquids are a promising class of novel antibiotics that could effectively inhibit bacterial growth and reduce strain fitness and virulence factor production in P. aeruginosa infections.
RESEARCH IN MICROBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Bernadette Lechner, Simone Hageneder, Katharina Schmidt, Mark P. Kreuzer, Rick Conzemius, Erik Reimhult, Ivan Barisi, Jakub Dostalek
Summary: The growth of surface-attached single-stranded DNA chains is monitored using an evanescent wave optical biosensor that combines surface plasmon resonance and optical waveguide spectroscopy. This technique allows for accurate measurement of the thickness, volume content, and density gradients of the DNA brushes in real time, providing important insights for sensitive and rapid assays in affinity-based biosensors.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Alireza Mashaghi, Roland van der Veen
Summary: The topological framework of circuit topology has been introduced to complement knot theory and help in understanding the physics of molecular folding. Circuit topology captures the arrangements of intra-chain contacts within a given folded linear chain and allows for the classification and comparison of chains. Polynomial invariants have been presented that are efficient and powerful enough to deal with any combination of soft and hard contacts.
Article
Multidisciplinary Sciences
Jose Ceniceros, Mohamed Elhamdadi, Alireza Mashaghi
Summary: Circuit topology uses the algebraic structure of quandles to classify and distinguish chain topologies, studying basic motifs and defining quandle/bondle coloring for them. The implications of circuit topology operations allow for building complex topologies from basic motifs using the quandle coloring approach.
Article
Biochemical Research Methods
Shokoofeh Maghari, Erik Reimhult, Parviz Ghezellou, Alireza Ghassempour
Summary: The present study evaluated the influence of surface properties of nanoparticles (NPs) on the intensity of mass signals in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and laser desorption/ionization mass spectrometry (LDI-MS) experiments. The results indicated that the surface ligands of NPs significantly enhanced the signal intensity based on electrostatic interaction and laser energy absorption in LDI-MS analyses. In MALDI-MS experiments, achieving colloidal stability of NPs in the matrix solution led to a significant signal improvement.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Instruments & Instrumentation
Tayebeh Saghaei, Erik Reimhult, Peter D. J. van Oostrum
Summary: We present a technique for rapidly changing the contents of a small sample chamber with minimal sample consumption. Our design eliminates dead volume and minimizes mixing, making it an exciting alternative to traditional solutions. It also functions as an efficient bubble trap.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Multidisciplinary Sciences
Anatoly Golovnev, Alireza Mashaghi
Summary: The art of tying knots is widely used in nature and applications, from scouting programs to engineering molecular knots. Knotting proteins like knotted proteins exhibit various cellular functions, with their entanglement providing thermal and kinetic stability. The design principles of naturally evolved molecular knots are not well understood, but circuit topology theory has recently been generalized to account for chain entanglement. This detailed understanding may be beneficial for molecular engineering.
Article
Chemistry, Multidisciplinary
Antonino Puglisi, Simone Bassini, Erik Reimhult
Summary: Cholesterol plays a crucial role in major cardiovascular and neurodegenerative diseases, and cyclodextrins have shown promising therapeutic efficacy in sequestering and mobilising cholesterol. A new approach involves core-shell superparamagnetic iron oxide nanoparticles functionalised with cyclodextrins, which can specifically cleave off from the nanoparticles' shell at a slightly acidic pH to mobilise cholesterol out of the lysosome. This platform shows potential in removing cholesterol from the lysosomal compartment.
FRONTIERS IN CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Katharina Schmidt, Simone Hageneder, Bernadette Lechner, Barbara Zbiral, Stefan Fossati, Yasaman Ahmadi, Maria Minunni, Jose Luis Toca-Herrera, Erik Reimhult, Ivan Barisic, Jakub Dostalek
Summary: This study tailored rolling circle amplification (RCA) for affinity biosensors relying on optical probing with confined surface plasmon field. By combining surface plasmon resonance (SPR) and surface plasmon-enhanced fluorescence (PEF) methods, the impact of long RCA-generated single-stranded DNA (ssDNA) chains on sensor response enhancement was investigated. The reported RCA implementation improved the limit of detection (LOD) for model target analyte, showing enhancement in sensor response and potential for single-molecule detection.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Antonino Puglisi, Noemi Bognanni, Graziella Vecchio, Ece Bayir, Peter van Oostrum, Dawn Shepherd, Frances Platt, Erik Reimhult
Summary: Core-shell superparamagnetic iron oxide nanoparticles have great potential as a therapeutic and diagnostic platform in biological systems. This study investigates the biological effects of multifunctional cyclodextrin-appended SPIONs (CySPION) in mutant Npc1-deficient CHO cells. The results show that CySPIONs have low cytotoxicity, are strongly endocytosed, and localized in the lysosomal compartment. The pH-sensitive chemistry of these nanoparticles allows for the release of monomeric cyclodextrins, promoting the removal of accumulated cholesterol. Additionally, CySPIONs demonstrate high transport across blood-brain barrier models, suggesting their potential as a therapeutic approach for cholesterol-related brain diseases.
Article
Microbiology
Guruprakash Subbiahdoss, Sarah Osmen, Erik Reimhult
Summary: The Komagataeibacter bacteria in kombucha can produce cellulosic biofilms using hydrocarbons as the sole source of carbon and energy. The presence of biosurfactants in kombucha leads to a faster decrease in interfacial tension and the formation of stable and elastic biofilm membranes.
Review
Medicine, Research & Experimental
Erik Reimhult, Mudassar Mumtaz Virk
Summary: Hybrid lipopolymer vesicles are membrane vesicles that can self-assemble on both the micro- and nanoscale, potentially serving as smart materials for drug delivery systems with different properties from their macroscale counterparts.
JOURNAL OF BIOMEDICAL RESEARCH
(2021)
Meeting Abstract
Biophysics
Peter van Oostrum, Erik Reimhult, Angelo Cacciuto, Tung Clarion, Alexander Mair, Ivan Coluzza
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
