期刊
ANALYTICAL BIOCHEMISTRY
卷 559, 期 -, 页码 51-54出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2018.08.013
关键词
Cell culture; iTRAQ; Quantitative proteomics; Biological variation
资金
- Fundacao Carlos Chagas Filho de Amparo a Pesquisa o Estado do Rio de Janeiro (FAPERJ)
- Conselho Nacional de Desenvolvimento Cientiico e Tecnologico (CNPq)
Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) < 20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.
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