期刊
ANALYTICAL BIOCHEMISTRY
卷 452, 期 -, 页码 16-18出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.01.005
关键词
Sample preparation; Boron nitride; Protein desalting; Hydrophilic peptides; Phosphoproteomics
资金
- EU-Marie Curie ITN MERIT [GA 2010-264474]
Hydrophilic peptides in shotgun proteomics have been shown to be problematic in conventional chromatography. Typically, C18 solid phase extraction or peptide traps are used for desalting the sample prior to mass spectrometry analysis, but the capacity to retain hydrophilic peptides is not very high, causing a bias toward more hydrophobic peptides. This is particularly problematic in phosphoproteomic studies. We tested the compatibility of commercially available boron nitride as a novel material for peptide desalting. Boron nitride can be used to recover a wide range of peptides with different physicochemical properties comparable to combined C18 and graphite carbon material. (C) 2014 Published by Elsevier Inc.
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