Article
Chemistry, Analytical
Yi Chen, Xiaowen Jiang, Junnan Wang, Zhengxiu Wu, Yuchen Wu, Zhonghua Ni, Hong Yi, Rongsheng Lu
Summary: This study proposed a scheme to use sorbitol to mitigate interference in NMR blood glucose detection method, enhancing the sensitivity of Fe2+ to H2O2 and reducing the impact of interfering substances on Fe2+/Fe3+ conversion, thus improving detection accuracy.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yu Liu, Yanan Cao, Cong Zhang, Cheng Ye, Qinglai Bian, Xue Cheng, Hui Xia, Junping Zheng, Hongtao Liu
Summary: This study proposes a novel colorimetric method using gold nanoparticles (AuNPs) for the rapid and visual detection of hydrogen peroxide (H2O2) and oxidase-specific substrates. The method demonstrates high specificity, high sensitivity, and tolerance to the influence of dissolved oxygen.
ANALYTICA CHIMICA ACTA
(2022)
Article
Polymer Science
Martina Scotti, Paolo Arosio, Elisa Brambilla, Salvatore Gallo, Cristina Lenardi, Silvia Locarno, Francesco Orsini, Emanuele Pignoli, Luca Pedicone, Ivan Veronese
Summary: This study investigates the influence of ferrous ammonium sulphate (FAS) and xylenol orange sodium salt (XO) concentrations on the sensitivity and linearity range of the PVA-GTA-FG dosimeters. The results show that the concentrations of FAS and XO determine the sensitivity to absorbed dose and the linearity interval of the dose-response curve. The study suggests that the best performance can be achieved with FAS concentrations of 1.00-0.40 mM and XO concentrations of 0.200-0.166 mM for spectrophotometric analyses of FG dosimeters.
Article
Public, Environmental & Occupational Health
Nancy B. Hopf, Jean-Jacques Sauvain, Samantha L. Connell, Nicole Charriere, Francesco Saverio Romolo, Guillaume Suarez
Summary: Hydrogen peroxide (H2O2) is commonly used in hair coloring, disinfecting, and bleaching processes, but it can cause airway irritation and inflammation. This study proposes two sensitive methods to measure H2O2 concentration and oxidative potential (OP) and evaluates them in different exposure scenarios. The results show a correlation between OP and H2O2 concentrations, and suggest the need for further development of direct reading methods for field applications.
ANNALS OF WORK EXPOSURES AND HEALTH
(2022)
Article
Engineering, Environmental
Yanting Zhang, Na Zhang, Ao Qian, Chenglong Yu, Peng Zhang, Songhu Yuan
Summary: This study reveals the significant influence of the C/Fe molar ratio on the production of hydrogen peroxide and hydroxyl radical during the oxygenation of reduced iron and natural organic matter in the subsurface. The accumulation of hydroxyl radical increases more prominently with the increase in the C/Fe ratio.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Applied
Qiuyue Ji, Fang Zhu, Yu Lei, Hao Cheng, Jianfeng Ma, Sridhar Komarneni
Summary: Two different visible light-assisted heterogeneous photocatalytic systems were investigated for degrading Orange II, with significant parameters such as BS or H2O2 concentration showing a significant effect on the degradation process. The BS/Fe-Co-SBA/Vis system was suitable for a wider pH range, while the H2O2/Fe-Co-SBA/Vis system was greatly influenced by the initial pH of the solution. Different free radicals were involved in the degradation process of Orange II in each system, as determined by ESR spectroscopy.
MICROPOROUS AND MESOPOROUS MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Chia-Chang Lin, Yu-Hong Zhong
Summary: In this study, nano-sized copper particles (RPB-nano-Cu0) synthesized by chemical reduction in a rotating packed bed (RPB) were used to degrade Orange G in water by the nano-Cu0/H2O2 process. The effects of H2O2 dosage and RPB-nano-Cu0 dose on the degradation of Orange G were investigated. The RPB-nano-Cu0/H2O2 process exhibited an optimum degradation efficiency of 93% for Orange G (10 mg/L) at pH 3, 25℃, H2O2 dosage of 5 mmol/L, and RPB-nano-Cu0 dose of 0.25 g/L after 30 minutes.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Medicine, Research & Experimental
Yi-Ching Cheng, Chao-Lin Kuo, Sheng-Yao Hsu, Tzong-Der Way, Ching-Ling Cheng, Jaw-Chyun Chen, Kuo-Ching Liu, Shu-Fen Peng, Wai-Jane Ho, Fu-Shin Chueh, Wen-Wen Huang
Summary: Pre-treatment of tetrandrine and treatment with H2O2 enhanced H2O2-induced cell apoptosis and DNA condensation in HaCaT cells, suggesting a potential approach for the treatment of proliferated malignant keratinocytes.
Article
Engineering, Chemical
Maroua Nessaibia, Houria Ghodbane, Ilhem Djaghout, Abdelhalim Fetimi, Slimane Merouani, Manawwer Alam, Marco Balsamo, Alessandro Erto, Yacine Benguerba
Summary: This study investigates the decomposition of Acid Orange 10 dye using the H2O2/IO4- reactive system and evaluates the impact of various factors on the process efficiency. It found that the concentrations of H2O2 and IO4- exert a nonmonotonic effect on the removal of AO10, and acidic conditions significantly promote the degradation efficiency. Moreover, an increase in salinity decreases the effectiveness of AO10 removal.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)