期刊
ANALYTICAL BIOCHEMISTRY
卷 448, 期 -, 页码 38-40出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.11.031
关键词
Acyl chain length; HPLC; Lipase; Multisubstrate; Substrate specificity; 4-Nitrophenol
资金
- Council of Scientific and Industrial Research, Government of India
Lipases and esterases are hydrolytic enzymes and are known to hydrolyze esters with unique substrate specificity and acyl chain length selectivity. We have developed a simple competitive multiple substrate assay for determination of acyl chain length selectivity of lipases/esterases using RP-HPLC with UV detection. A method for separation and quantification of 4-nitrophenyl fatty acid esters (C-4-C-18) was developed and validated. The chain length selectivity of five lipases and two esterases was determined in a multisubstrate reaction system containing equimolar concentrations of 4-nitrophenyl esters (C-4-C-18). This assay is simple, reproducible, and a useful tool for determining chain length selectivity of lipases/esterases. (C) 2013 Elsevier Inc. All rights reserved.
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