Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Eva Cifkova, Marcus Horing, Ding Mei, Michaela Chocholouskova, Ondrej Peterka, Jakub Idkowiak, Tereza Hrnciarova, Ladislav Kuchar, Robert Ahrends, Radana Brumarova, David Friedecky, Gabriel Vivo-Truyols, Pavel Skrha, Jan Skrha, Radek Kucera, Bohuslav Melichar, Gerhard Liebisch, Ralph Burkhardt, Markus R. Wenk, Amaury Cazenave-Gassiot, Petr Karasek, Ivo Novotny, Kristina Greplova, Roman Hrstka, Michal Holcapek
Summary: Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients. The study found significant differences in blood lipids between pancreatic cancer patients and healthy controls.
NATURE COMMUNICATIONS
(2022)
Article
Food Science & Technology
Fan Liu, Jan van der Molen, Folkert Kuipers, Sander S. van Leeuwen
Summary: Human milk is the best food for infants, while infant formulas are appropriate substitutes. This study analyzed the concentrations of bioactive components in different infant formulas available on the Dutch market and found that they were lower than in human milk.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Xuelei Chen, Nan Zhang, Liping Li, Rong Zhao, Ning Chen, Sai Fan, Zhixiong Shi
Summary: A novel and simple procedure was developed to simultaneously determine 14 OPEs and 6 diester metabolites in dairy products and human milk. The study found high detecting frequencies of OPEs and their metabolites in real samples, indicating the ubiquitous presence of these emerging pollutants in foods and the human body. The impact of the diester metabolites on population exposure must be included in exposure assessment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Jiaqi Li, Ye Bi, Yi Zheng, Cuiyan Cao, Long Yu, Zhenyu Yang, Wengang Chai, Jingyu Yan, Jianqiang Lai, Xinmiao Liang
Summary: A high-throughput method was developed for the accurate quantitation of 23 complex human milk oligosaccharides. The method was fast and reliable, and was validated through a large population survey of mothers from different ethnic groups in China.
Article
Environmental Sciences
Nikita Bakanov, Carolina Honert, Lisa Eichler, Gerlind U. C. Lehmann, Ralf Schulz, Carsten A. Bruehl
Summary: A simple acetonitrile-based extraction method using HPLC-ESI-MS/MS was developed for the determination of 98 current-use pesticides in soil and herbaceous vegetation. The method exhibited good accuracy and precision, with percentage recoveries ranging from 71-125% (soil) and 70-117% (vegetation), and relative standard deviations of 1-14% (soil) and 1-13% (vegetation). Matrix-matched calibration curves showed excellent linearity (R-2 > 0.99). The method was successfully applied to agricultural sites in Germany, where 44 of the 98 pesticides were detected, indicating a high qualitative load compared to EU arable soils.
Article
Food Science & Technology
Yung-Husan Chen, Yu-Wei Chang, Chu-Wen Ma, Lian-Zhong Luo, Ting-Jang Lu, Jeng-Yuan Yao
Summary: This study analyzed the bioactive compounds of cultured three-spot seahorse using HPLC-ESI/MS/MS and evaluated their biological activities. Taurine and arginine were identified as the primary bioactive compounds, with the water layer extract containing the highest content. Additionally, the water layer extract demonstrated anti-inflammatory activity.
FOOD SCIENCE & NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Yinzhi Lang, Yongzhen Zhang, Chen Wang, Limei Huang, Xiaoxiao Liu, Ni Song, Guoyun Li, Guangli Yu
Summary: Human milk oligosaccharides (HMOs) have various biological activities for infants, making them complex to analyze, but 2-AA labeling technique showed the most consistent deprotonated molecular ions, enhanced sensitivity, least structural selectivity, and sequencing-informative tandem MS fragmentation spectra. This technique was selected for further optimization to provide resolute detection of many HMOs under PGC-MS conditions.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Applied
Christof B. Steingass, Jonas Burkhardt, Vicky Baeumer, Keshav Kumar, Heiko Mibus-Schoppe, Jana Zinkernagel, Patricia Esquivel, Victor M. Jimenez, Ralf Schweiggert
Summary: Anthocyanins were characterized in red cabbage, sweet potato, and Tradescantia pallida leaves. Various non-, mono-, and diacylated cyanidins were identified in red cabbage. Sweet potato leaves contained mono- and diacylated cyanidin- and peonidin glycosides, while T. pallida leaves mainly contained tetra-acylated anthocyanin tradescantin. The thermal stability of red cabbage and purple sweet potato extracts surpassed that of a commercial food dye, but still fell short compared to the stability of Tradescantia extract.
Article
Agriculture, Multidisciplinary
Rita Petrucci, Paola Di Matteo, Anatoly P. Sobolev, Loredana Liguori, Donatella Albanese, Noemi Proietti, Martina Bortolami, Paola Russo
Summary: Beer antioxidants mainly come from malts, and the phenolic antioxidant content and types in Pale Ale beers are influenced by the percentage of dark malt used. Dealcoholization by osmotic distillation has a weak impact on the phenolic fraction, with the content and antioxidant activity of phenolic compounds in beers primarily influenced by the type of malt used.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Huipeng Liu, Xiaoyu Wei, Shuyu Zu, Xihuang Lin, Jinchuang Zhang, Aimin Shi, Qiang Wang, Ning He
Summary: Plant-derived neutral oligosaccharides and pectic-oligosaccharides have potential as prebiotics due to their DP and structural complexity. In this study, neutral oligosaccharides were produced from apple pectin, refined through membrane separation, and found to promote the growth of selected Lactobacillus strains. The refined neutral oligosaccharides were shown to have a main oligosaccharide structure, making them effective prebiotics for potential application in functional food.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Malk Eun Pak, Yeon-Ji Kim, Yeo Jin Park, Younghoon Go, Chul Soo Shin, Jong-Won Yoon, Seon-min Jeon, Young-Ha Song, Kyungho Kim
Summary: The ingestion of human milk oligosaccharides (HMOs) has been linked to a lower risk of inflammatory diseases and infections, but their role in platelet aggregation and thrombus formation is unclear. This study found that 2'-fucosyllactose (2'-FL), a type of HMO, has antiplatelet and antithrombotic effects by inhibiting platelet aggregation and cell activation, and it can protect against arterial thrombus formation and stroke.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Pharmacology & Pharmacy
Seung-Hyun Jeong, Ji-Hun Jang, Hea-Young Cho, Yong-Bok Lee
Summary: This study compared pharmacokinetic parameters obtained using two newly developed assays, HPLC-UV and UPLC-ESI-MS/MS, and confirmed their reliability and accuracy. Results showed that both methods met the criteria of international guidance and could be successfully applied to PK studies. Comparison of PK parameters between the two assays revealed differences in predicted minimum plasma concentrations, which may impact clinical dose and usage settings.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Mekonnen Tilahun, Liansheng Zhao, Lingling Sun, Yifan Shen, Lu Ma, Todd R. Callaway, Jianchu Xu, Dengpan Bu
Summary: This study investigated the effects of adding fresh amla fruit to the diet of lactating dairy cows. The results showed that amla fruit supplementation improved the antioxidant capacity and fatty acid proportions of milk, which can contribute to better quality and production of dairy products.
Article
Biochemical Research Methods
Qianru Ma, Wentao Wang, Xu Yang, Yunhong Chen, Yan Liu, Hongxu Chen, Yufen Zhao
Summary: This study developed a novel glycan labelling reagent, P(4)HZD, for mass spectrometry analysis of oligosaccharides. The reagent with a permanent positive charge enhanced the MS signal intensity of oligosaccharides. Compared with a commercialized oligosaccharide tag, P(4)HZD more effectively enhanced the signal of oligosaccharides in the middle or higher mass range using both ESI and MALDI ion sources. P(4)HZD showed good efficacy for the detection of oligosaccharides in complex biological samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Tamara Fernandez-Bautista, Beatriz Gomez-Gomez, Roberto Palacin-Garcia, Emma Gracia-Lor, Teresa Perez-Corona, Yolanda Madrid
Summary: The distribution of Se and Hg in different protein fractions extracted from muscle tissues of tuna, swordfish, and salmon (wild vs. farmed) was investigated for the first time. The study revealed that Se and Hg were associated with proteins of specific molecular weights, with differences observed between farmed and wild salmon. Additionally, Se speciation studies showed SeMeSeCys as the primary selenium species found in the proteins analyzed.
Article
Obstetrics & Gynecology
Nicola Volpi, Francesca Maccari, Fabio Galeotti, Chiara Peila, Alessandra Coscia, Lucia Zampini, Chiara Monachesi, Orazio Gabrielli, Giovanni Coppa
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2020)
Article
Food Science & Technology
Maira Zorzan, Daniela Collazuol, Giovanni Ribaudo, Alberto Ongaro, Carla Scaroni, Giuseppe Zagotto, Decio Armanini, Susi Barollo, Fabio Galeotti, Nicola Volpi, Marco Redaelli, Raffaele Pezzani
JOURNAL OF FUNCTIONAL FOODS
(2019)
Article
Biochemistry & Molecular Biology
Hajer Bougatef, Fatma Krichen, Federica Capitani, Ikram Ben Amor, Jalel Gargouri, Francesca Maccari, Veronica Mantovani, Fabio Galeotti, Nicola Volpi, Ali Bougatef, Assaad Sila
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Chemistry, Physical
Vincenzo Zaccaria, Emanuele Ugo Garzarella, Carmen Di Giovanni, Fabio Galeotti, Lucia Gisone, Davide Campoccia, Nicola Volpi, Carla Renata Arciola, Maria Daglia
Article
Infectious Diseases
Rachele Francese, Andrea Civra, Manuela Donalisio, Nicola Volpi, Federica Capitani, Stefano Sottemano, Paola Tonetto, Alessandra Coscia, Giulia Maiocco, Guido E. Moro, Enrico Bertino, David Lembo
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Biochemical Research Methods
Fabio Galeotti, Federica Capitani, Francesca Maccari, Veronica Mantovani, Nicola Volpi
Summary: This study presents capillary electrophoresis (CE) separation of Artepillin C in Brazilian propolis, demonstrating its advantages over HPLC-UV-MS method in terms of higher sensitivity, shorter run time, stronger robustness, and better reproducibility.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2021)
Article
Chemistry, Analytical
Nicola Volpi, Fabio Galeotti, Francesca Maccari, Federica Capitani, Veronica Mantovani
Summary: The study investigated the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples obtained from different biological sources. The results showed that highly pure CS products with consistent structure and characteristics could be obtained through standardized manufacturing processes. Different origin sources of CS exhibited variations in molecular weight, sulfate groups ratio, and the presence of co-purified molecules.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Davide Tamburro, Sinisa Bratulic, Souad Abou Shameh, Nikul K. Soni, Andrea Bacconi, Francesca Maccari, Fabio Galeotti, Karin Mattsson, Nicola Volpi, Jens Nielsen, Francesco Gatto
Summary: The study characterized the analytical performance of an UHPLC-MS/MS-based kit for quantification of 17 free GAG disaccharides, showing acceptable linearity, selectivity, specificity, accuracy, and precision in quantifying 15 disaccharides. In native human samples, the kit demonstrated acceptable analytical performance for the quantification of CS disaccharides. Intra- and inter-laboratory tests confirmed robust reproducibility of GAG measurements, indicating that the kit was adequately standardized for simultaneous measurement of free GAG disaccharides.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Pediatrics
Rachele Francese, Manuela Donalisio, Massimo Ritta, Federica Capitani, Veronica Mantovani, Francesca Maccari, Paola Tonetto, Guido E. Moro, Enrico Bertino, Nicola Volpi, David Lembo
Summary: HM-GAGs have significant anti-HCMV and anti-RSV activity and alter virus binding to the cell. The contribution of individual HM-GAGs depends on their structure, rather than a simple charge interaction between the virus and sulfate groups. The antiviral activity of HM-GAGs does not change after thermal treatment.
PEDIATRIC RESEARCH
(2022)
Article
Biochemical Research Methods
Francesca Maccari, Nicola Volpi
Summary: Chondroitin sulfate (CS) with different molecular weights was tested using uronic acid carbazole assay and cetylpyridinium chloride (CPC) titration, showing that the measurement values decrease with the increase of CS molecular weight. It is important to know the molecular weight values and use comparable standards before applying the assays to obtain reliable results.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Nicola Volpi, Veronica Mantovani, Fabio Galeotti, Davide Bianchi, Valentina Straniero, Ermanno Valoti, Niccolo Miraglia
CLINICAL PHARMACOLOGY IN DRUG DEVELOPMENT
(2019)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
