期刊
ANALYTICAL BIOCHEMISTRY
卷 421, 期 1, 页码 339-341出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.10.033
关键词
Concanavalin A-magnetic particle conjugates; Glycoproteomics; Native cell membrane glycoproteins; Mass spectrometry
资金
- National Natural Science Foundation of China [30870549]
- Chinese Ministry of Science and Technology [2009DFA32730]
The discovery, isolation, and subsequent identification of cell membrane glycoproteins involved in the structure and function of the cell surface are becoming more and more important. Here, concanavalin A-magnetic particle conjugates were employed to isolate the special membrane glycoproteins from living HepG-2 cells. The isolated glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry as well as annotated. A total of 37 membrane glycoproteins were identified, and 25 of them were ascertained to locate in the extracellular region. (C) 2011 Elsevier Inc. All rights reserved.
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