Article
Multidisciplinary Sciences
Julian Ihssen, Nina Jovanovic, Teja Sirec, Urs Spitz
Summary: Adenosine triphosphate (ATP) is a crucial indicator of cell viability. Real-time monitoring of extracellular ATP (eATP) release by bacteria using engineered luciferase can provide insights into bacterial physiology and susceptibility to antibiotics. This method offers faster and more sensitive antimicrobial susceptibility testing compared to conventional methods.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Elisa Michelini
Summary: Bioluminescence has become an essential tool in various applications such as bioassays, biosensors, and in vivo imaging. This study focuses on the development of new luciferases with optimized properties, including improved thermostability, pH-insensitive emission, high quantum yield, extended kinetics, and red-shifted emission. Two new synthetic luciferases were designed and characterized, showing promising features as reporter proteins and bioluminescent probes. As a proof-of-principle application, an inflammation assay using 3D cell cultures was developed, demonstrating the analytical performance of the luciferases.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Elisa Michelini
Summary: Bioluminescence, the emission of light in living organisms, is an important tool for various applications such as bioassays and biosensors. Recent advancements in the development of synthetic luciferases have led to improved properties for in vitro and in vivo biosensing. In this study, two new synthetic luciferases were designed and characterized, and their potential applications were demonstrated through an inflammation assay with promising results.
Article
Chemistry, Analytical
Elham Karimi, Maryam Nikkhah, Saman Hosseinkhani
Summary: Using gold nanoparticles as an enzyme immobilizer can increase the activity of luciferases and improve signal stability in the presence of nanoparticles. The application of ATP in the firefly luciferase reaction increases luciferase activity and enhances detection limits.
Article
Biophysics
Zhen Zheng, Tiantian Xia, Tao Wang, Deying Jia, Ziye Wu, Jun Jiang, Gaolin Liang
Summary: By realizing the firefly bioluminescence cycle in vitro and in cells, researchers have identified the optimal chemical route to achieve the highest light intensity. This pioneering study provides the opportunity to design tunable, economical, biomimetic cold light devices in the near future.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Mu-Shen Chang, Chia-Yi Lee, En-Shuo Liu, Hsuan Chao, Hsin-Yu Wu, Yu-Yen Chang, Yen-Ling Liu, Yu-Tung Chen, Yu-Cheng Su, Yeng-Tseng Wang, Tian-Lu Cheng, Chia-Hung Yen, Cheng-Wei Lin, Hsin-Kai Huang, Wen-Wei Lin
Summary: Researchers have developed a new gene reporter system called mHRP, which uses a cell membrane-expressed HRP to preserve enzymatic activity on the cell surface, avoiding cell lysis and the use of expensive luciferase substrates. The mHRP system provides stable detection signals and has a longer substrate half-life.
ANALYTICAL CHEMISTRY
(2023)
Letter
Chemistry, Medicinal
Yunzhi Li, Chaoying Jin, Huiying Xu, Weijian Wu, Youqiao Wang, Jiaxin Wu, Tingyu Liu, Guohui Wan, Xin Yue, Xianzhang Bu
Summary: Highly potent and reversible Firefly luciferase inhibitors were identified, which have significant applications in constructing novel assay platforms and efficiently blocking the bioluminescence generated by Fluc in vivo.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Review
Chemistry, Analytical
Elena Efremenko, Olga Senko, Nikolay Stepanov, Olga Maslova, Galina Yu. Lomakina, Natalia Ugarova
Summary: Bioluminescent ATP-assay has a wide range of applications in various fields, including sanitary control, water quality monitoring, food industry, drug maintenance, and monitoring of biocatalysts in biotechnological processes. New areas of ATP sensing, such as synthetic microbial consortia, pesticide degradation, and control of biocorrosive processes, have also been developed.
Review
Chemistry, Multidisciplinary
Aisha J. Syed, James C. Anderson
Summary: Bioluminescence is a natural phenomenon where living creatures produce light through a reaction between luciferin and luciferase. Various luciferin-luciferase pairs have different applications, such as gene assays and drug discovery. In recent years, bioluminescence has found increasing applications in biomedicine, including photodynamic therapy and neuron control.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Sung-Bae Kim, Ryo Nishihara, Ramasamy Paulmurugan
Summary: We identified the best resonance energy donor for near infrared bioluminescence resonance energy transfer (NIR-BRET) templates and achieved high sensitivity in monitoring molecular events in cells. The established templates, fused with fluorescent proteins, showed a large blue-to-red shift and were used in new NIR-BRET systems for imaging steroid hormone activities. The NIR-BRET systems demonstrated specific luminescence signals upon exposure to different steroid hormones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Shufeng Li, Zhiyang Ruan, Hang Zhang, Haiwei Xu
Summary: Bioluminescence imaging (BLI) is a newly developed noninvasive visual approach that utilizes the firefly luciferin-luciferase system to achieve high sensitivity imaging of various biological processes, with applications in tumor activity evaluation, disease detection, and therapy.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Yuki Ohmuro-Matsuyama, Keiko Gomi, Takuya Shimoda, Hideki Yamaji, Hiroshi Ueda
Summary: The study developed a protein-protein interaction assay, FlimPIA, based on mutant firefly luciferases, improving the stability and sensitivity of the assay system through strain modification and mutant screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jittima Phonbuppha, Ruchanok Tinikul, Yoshihiro Ohmiya, Pimchai Chaiyen
Summary: In this study, we constructed a gene reporter system based on Flavin Luciferase from Vibrio campbellii (Vc) (FLUXVc), which can generate powerful bioluminescence signals in mammalian cells. The signal intensity of FLUXVc is over 400-fold higher than the nonengineered version.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Namdoo Kim, Seunghan Shin, Se Won Bae
Summary: cAMP serves a crucial role in signal transduction pathways, with research focusing on its dynamics leading to insights for drug development and disease treatment. To enable real-time non-invasive imaging, genetically-encoded sensors based on fluorescent proteins and luciferases have been developed as powerful tools.
Article
Chemistry, Analytical
Hector Martinez-Perez-Cejuela, Denise Gregucci, Maria Maddalena Calabretta, Ernesto Francisco Simo-Alfonso, Jose Manuel Herrero-Martinez, Elisa Michelini
Summary: The identification of new strategies to improve protein stability is crucial for various applications. Metal-organic frameworks (MOFs) have been found to be versatile host platforms for protein immobilization, providing protection in harsh conditions. In this study, a thermostable luciferase mutant was used as a model to investigate the suitability of MOFs to enhance stability and enable real-world applications such as ATP detection in portable systems. The results demonstrated that the use of MOF significantly improved the stability of luciferase under adverse conditions, and the biocomposite outperformed the free enzyme in terms of ATP detection sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)