Article
Biochemistry & Molecular Biology
Negin Gooran, Bo Kyeong Yoon, Joshua A. Jackman
Summary: This study comparatively evaluated the membrane-disruptive properties of TX-100 and potential replacement SL-11W using a supported lipid bilayer (SLB) platform. The results showed that TX-100 exhibited higher potency and caused rapid and irreversible membrane solubilization, while SL-11W induced more gradual and reversible membrane budding. The study also revealed that both TX-100 and SL-11W showed concentration-dependent interaction behaviors and were only active at or above their critical micelle concentration (CMC) values.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Siddhartha Banerjee, Yuri L. Lyubchenko
Summary: The article discusses the preparation of smooth, stable, and structurally homogeneous lipid bilayers without trapped vesicles, for probing the time-dependent early stages of aggregation of monomeric amyloid proteins. This methodology development allows for monitoring amyloid protein aggregation at low protein concentrations, and for studying the specific role of different lipids in aggregation kinetics and disease development mechanisms.
Article
Polymer Science
Nisha Hollingsworth, Ronald G. Larson
Summary: This study investigates the swelling/deswelling and hysteresis behavior of weakly ionizable polyanion poly(acrylic acid) (PAA) brushes and bilayers containing PAA and poly(ethylene imine) (PEI) using a quartz crystal microbalance with dissipation (QCM-D) system. The results demonstrate that the brush height is influenced by grafting density, chain length, ion charge distribution, and pH value, leading to hysteresis phenomena under different conditions. The findings highlight the complex thermodynamics and kinetics of polyelectrolyte films, even at the monolayer and bilayer level.
Article
Materials Science, Biomaterials
Po-Ying Yeh, Jia-Yang Chen, Mo-Yuan Shen, Ting-Fang Che, Syer Choon Lim, Jocelyn Wang, Wen-Sy Tsai, Curtis W. Frank, Chun-Jen Huang, Ying-Chih Chang
Summary: Due to the scarcity, vulnerability, and heterogeneity of circulating tumor cells (CTCs), the existing CTC isolation system based on immunoaffinity interaction lacks consistent efficiency. The newly developed LIPO-SLB platform, incorporating a chaotic-mixing microfluidic system and antibody-conjugated liposome-tethered-supported lipid bilayer, offers high CTC capture efficiency, viability, and selectivity. The platform has been successfully validated using different cancer cell lines and clinical samples, demonstrating its enormous potential for prognostic and predictive purposes in precision medicine.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biochemistry & Molecular Biology
Noel F. Bonet, Daniel G. Cava, Marisela Velez
Summary: Quartz Crystal Microbalance (QCM) with dissipation and Atomic Force Microscopy (AFM) are two characterization techniques that provide kinetic, thermodynamic, and structural information at the nanometer scale of events taking place at solid-liquid interfaces. They are used to describe biological events at supported lipid bilayers (SLBs).
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Multidisciplinary
J. Tucker Andrews, Kirstyn E. Baker, Jacob T. Handloser, Natalie Bridges, Alexis A. Krone, Peter J. N. Kett
Summary: This study used a quartz crystal microbalance to investigate the effect of salt on the formation of phosphatidylcholine SLBs on a silicon dioxide surface, finding that the influence of different cations on SLB formation varies at different salt concentrations.
Article
Multidisciplinary Sciences
Mantas Ziaunys, Andrius Sakalauskas, Kamile Mikalauskaite, Vytautas Smirnovas
Summary: Protein aggregation is considered a major cause of several neurodegenerative diseases. Despite progress in amyloid research, the process of fibrillization remains not fully understood. Tracking insulin aggregation using ThT reveals anomalous double-sigmoidal kinetics, likely related to ThT-positive intermediates, which do not significantly alter the overall aggregation process.
Article
Materials Science, Multidisciplinary
Shova Neupane, Kai Betlem, Frank Uwe Renner, Patricia Losada-Perez
Summary: Solvent-assisted lipid bilayer (SALB) formation is a versatile approach for creating supported lipid membranes (SLBs) on metal surfaces, with lipid concentration in the solvent being a key parameter. The study found that at low concentrations, mostly monolayers are present, while at high concentrations, diverse structures coexist. The mechanism of SALB formation leads to unique two-step features in approach force curves on SLBs.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Deepika Regmi, Fengyun Shen, Aleksander Stanic, Majedul Islam, Deguo Du
Summary: Misfolding and aggregation of cellular prion protein (PrPc) is a major molecular process involved in the pathogenesis of prion diseases. The aggregation of PrP(106-128) depends on concentration, pH, preformed amyloid seeds, and the lipid composition and rigidity of liposomes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Chemistry, Analytical
Luigi Quercia, Iuliia Khomenko, Rosamaria Capuano, Matteo Tonezzer, Roberto Paolesse, Eugenio Martinelli, Alexandro Catini, Franco Biasioli, Corrado Di Natale
Summary: Dynamic headspace extraction is commonly used in gas sensor measurements. This study found that the kinetics of volatile organic compounds (VOCs) is diverse, while sensor signals exhibit a contrasting behavior with the concentration of most VOCs. The information content of sensor signals decays as their values continue to rise, suggesting that measurement protocols may need to be adjusted.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Katerina Konstantoulea, Patricia Guerreiro, Meine Ramakers, Nikolaos Louros, Liam D. Aubrey, Bert Houben, Emiel Michiels, Matthias De Vleeschouwer, Yulia Lampi, Luis F. Ribeiro, Joris Wit, Wei-Feng Xue, Joost Schymkowitz, Frederic Rousseau
Summary: The study found that proteins enriched in homologous sequences to Aβ aggregation-prone regions are present in Aβ plaques from AD patients, suggesting heterotypic amyloid interactions may occur. These proteins can modify Aβ assembly kinetics, fibril morphology, and deposition pattern in vitro. Additionally, it was discovered that transient expression of three of these proteins in an Aβ reporter cell line promotes Aβ amyloid aggregation, indicating a potential role of heterotypic APR interactions in amyloid-deposition diseases.
Article
Clinical Neurology
Lei Liu, Hyunchang Kwak, Trebor L. Lawton, Shan-Xue Jin, Angela L. Meunier, Yifan Dang, Beth Ostaszewski, Alison C. Pietras, Andrew M. Stern, Dennis J. Selkoe
Summary: The study developed a new method for detecting and quantifying soluble oligomers of amyloid beta protein in human plasma. The method is highly sensitive, cost-effective, and suitable for high throughput analysis.
ALZHEIMERS & DEMENTIA
(2022)
Article
Biochemistry & Molecular Biology
Panagiotis M. Spatharas, Georgia I. Nasi, Paraskevi L. Tsiolaki, Marilena K. Theodoropoulou, Nikos C. Papandreou, Andreas Hoenger, Ioannis P. Trougakos, Vassiliki A. Iconomidou
Summary: Clusterin is identified as a glycoprotein involved in amyloid formation and has aggregation-prone regions that can form amyloid-like fibrils while also inhibiting amyloid-beta fibril formation. These findings suggest a potential role of clusterin in the molecular mechanism of inhibiting amyloid formation and indicate a possible involvement of molecular chaperones with amyloidogenic properties in regulating amyloid formation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Chemistry, Medicinal
Misato Tajiri, Ryo Yamada, Mayumi Hotsumi, Koki Makabe, Hiroyuki Konno
Summary: This study describes the total synthesis of berberine and selected analogues, and evaluates their effectiveness as amyloid beta (Aβ) aggregation inhibitors. The key step in the synthesis is the assembly of the berberine framework using an intermolecular Heck reaction. Berberine analog 17, incorporating a tertiary amine moiety, demonstrated good anti-Aβ aggregation activity, water solubility, and minimal toxicity to nerve cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
J. Bompard, O. Maniti, R. Aboukhachfe, D. Ausserre, A. Girard-Egrot
Summary: BALM is a novel wide-field optical microscopy technique that allows for real-time quantitative monitoring and high-resolution visualization of tBLM formation, providing new insights into the process and demonstrating great potential in the study of complex biological systems.
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)